Abstract: Objective: To evaluate the influence of continuous or intermittent force on stability of titanium microscrews. Methods: 144 microscrews were inserted bilaterally in intraradicular zones of maxillary first molar and second premolar in 36 beagles. Loads were delivered consecutively in continuous group (n=12), in cycles of 12 on/paused for 12h in intermittent group A (n=12), and in cycles of 24 on/paused for 24h in intermittent group B (n=12). The on/off cycles were repeated for 1, 3, 5 or 7 weeks, after which the animals were sacrificed, and microscopic computerized tomography (μCT) and pullout test were applied. Results: The μCT parameters of microscrews in all three groups increased gradually with loading time. Fmax increased and reached a peak at week 5 but dropped slightly at week 7. In continuous group, all measurements were lower than those in intermittent groups at all time points. All values in intermittent group A were higher than those in intermittent group B. Conclusion: An intermittent loading regimen appears to be more favorable for obtaining stability than continuous loading, and a 12 h/12 h on/off loading cycle is superior to a 24 h/24 h on/off protocol in promoting bone-implant contact.