Journal of Oral Science Research

Journal of Oral Science Research ›› 2016, Vol. 32 ›› Issue (4): 322-325.DOI: 10.13701/j.cnki.kqyxyj.2016.04.002

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Effect of BMP7 on Osteogenic Differentiation of Mouse Induced Pluripotent Stem Cells in 3D Culture System.

XU Ran1, ZHANG Yu2#, XING Jian-yu1, WANG Yi-bo1, DING Chao1, LV Jia-wei2, WEI Ren-yue2, SHI Jiu-hui1*, LIU Zhong-hua2*.   

  1. 1. The First Affiliated Hospital to Harbin Medical University, Harbin 150006, China;
    2. College of Life Science, Northeast Agricultural University, Harbin 150030, China
  • Received:2015-10-13 Online:2016-04-28 Published:2016-04-28

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Abstract: Objective: To investigate the effect of BMP7 on the osteogenic differentiation ability of mouse iPSCs in the absorbable gelatin sponge scaffold. Methods: EBs induced from mouse iPSCs were digested into single cells and microinjected into the gelatin sponge. After 7 days of osteogenic induction, the osteogenic and adhesive abilities of the cells were examined. Results: Mouse iPSCs attached and spread along the gelatin sponge. Compared with the group without BMP7 treatment, the group treated with BMP7 had higher levels of deposited calcium and osteoblastic markers. Conclusion: Gelatin sponge can be used as cell culture scaffold for mouse iPSCs and BMP7 promotes osteogenesis of mouse iPSCs in the culture system.

Key words: Mouse

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