Expression of RB1CC1 in the Occurrence and Development of Oral Squamous Cell Carcinoma of Human and Mouse.

doi:10.13701/j.cnki.kqyxyj.2017.03.002

Abstract

Abstract: Objective: To compare the expression of RB1-inducible coiled-coil 1 (RB1CC1) in normal oral mucosa, dysplasia and oral squamous cell carcinoma (OSCC) of human and mouse model, and explore the role of RB1CC1 in the occurrence and development of OSCC. Methods: Immunohistochemical and reverse transcription-polymerase chain reaction method were used to detect the expression of RB1CC1 in 10 cases of human normal oral mucosa, 20 cases of dysplasia and 20 cases of well differentiated OSCC as well as 7 cases of mouse normal oral mucosa, 13 cases of dysplasia and 13 cases of OSCC, respectively. Results: In human, the positive expression rates of RB1CC1 in dysplasia and well differentiated squamous cell carcinoma group were higher than that in normal oral mucosa group (P<0.05). In mouse, the positive expression rate of RB1CC1 gradually increased from normal to carcinoma group (P<0.05). By contrast, in human, the gene expression of RB1CC1 in normal oral mucosa was higher than dysplasia and OSCC group (P<0.05), but not significantly different in dysplasia and well differentiated OSCC group. Conclusion: The expression of RB1CC1 was similar in the same pathological stages of between human and mouse, and may play an important role in early carcinogenesis of OSCC.

Key words: RB1-inducible coiled-coil 1(RB1CC1)Oral squamous cell carcinoma, Human, Mouse

CLC Number:

R739.8

CHEN Lei, YU Da-hai, BI Shi-chang, QING Hai-yun, SHI Qiang, SUN Ying.. Expression of RB1CC1 in the Occurrence and Development of Oral Squamous Cell Carcinoma of Human and Mouse.[J]. Journal of Oral Science Research, 2017, 33(3): 240-243.

References

[1] Schmidt-Kittler O, Ragg T, Daskalakis A, et al. From latent disseminateal cells to overt metastasis: genetic analysis of systemic breast cancer progression [J]. Proc Natl Acad Sci USA, 2003, 100(13)∶7737-7742
[2] Li J, Liang F, Yu D, et al. Development of a 4-nitroquinoline- 1-oxide model of lymph node metastasis in oral squamous cell carcinoma [J]. Oral Oncology, 2013, 49(4)∶299-305
[3] Chano T. Application of novel Clinicopathological biomarkers [J]. Rinsho Byori, 2012, 60(2)∶167-173
[4] Hama Y, Chano T, Inui T, et al. Preparation of Mouse Monoclonal Antibody for RB1CC1 and its Clinical Applicaion [J]. PLos One, 2012, 7(3)∶e32052
[5] 王倩,马金宝,白桦.自噬相关蛋白LC3Av1在口腔鳞状细胞癌中表达及甲基化对其影响[J].口腔医学研究,2015,31(4)∶373-376
[6] Lebovitz CB, Robertson AG, Goya R, et al. Cross-cancer profiling of molecularalterations within the human autophagy interaction network [J]. Autophagy, 2015, 11(9) ∶1668-1687
[7] 常珍,李容林,李春阳.细胞自噬在口腔扁平苔藓恶变中作用的研究进展[J].国际口腔医学杂志,2012, 39(3)∶416-420
[8] Chen N, Debnath J. Autophagy and tumorigenesis [J]. FEBS Lett, 2010, 584(4)∶1427-1435
[9] Wei H, Wei S, Gan B, et al. Suppression of autophagy by FIP200 deletion inhibits mamary tumorigenesis [J]. Autophagy, 2011, 25(14)∶1510-1527
[10] Paun BC, Cheng Y, Leggett BA, et al. Screening for microsatellite instability identifies frequent 3'-Untranslated region mutation of the RB1-Inducible coiled-coil 1 gene in colon tumors [J]. Plos One, 2009, 4(11)∶e7715
[13] 施强,牟云,于大海,等.小鼠口腔癌模型骨髓播散细胞RB1CC1纯合性缺失的初步研究[J].中国临床新医学,2015,8(5)∶394-397
[14] Suraneni MV, Moore JR, Zhang D, et al. Tumor-suppressive functions of 15-Lipoxygenase-2 and RB1CC1 in prostate cancer [J]. Cell Cycle, 2014, 13(11)∶1798-1810
[15] Leidal AM, Debnath J. 'Doubling down' on the autophagy pathway to suppress tumor growth [J]. Genes Dev, 2014, 28(11)∶1137-1139
[16] Wei H, Guan J. Pro-tumorigenic function of autophagy in mammary oncogenesis [J]. Autophagy, 2012, 8(1)∶129-131

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