口腔医学研究

口腔医学研究 ›› 2016, Vol. 32 ›› Issue (4): 343-346.DOI: 10.13701/j.cnki.kqyxyj.2016.04.007

• 基础研究论著 • 上一篇    下一篇

TNF-α对根尖乳头干细胞体外增殖及成牙成骨向分化能力的影响

于莉,李淑慧,袁萍,赵璐,周春梅,吴佩玲*   

  1. 新疆医科大学第二附属医院口腔科 新疆 乌鲁木齐 830063
  • 收稿日期:2015-10-13 出版日期:2016-04-28 发布日期:2016-04-28
  • 通讯作者: 吴佩玲,E-mail:wplkq@sina.com
  • 作者简介:于莉(1989~ ),女,山东人,硕士在读,主要从事口腔内科的临床研究工作。
  • 基金资助:
    新疆维吾尔自治区自然科学基金(编号:2015211C107)

Effects of TNF-α on the Proliferation and Odontoblastic as well as Osteogenic Differentiation of SCAP in Vitro.

YU Li, LI Shu-hui, YUAN Ping, ZHAO Lu, ZHOU Chun-mei, WU Pei-ling*.   

  1. Department of Stomatology, the 2nd Affiliated Hospital of Xinjiang Medical University, Urumchi 830063, China
  • Received:2015-10-13 Online:2016-04-28 Published:2016-04-28

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摘要： 目的:探讨肿瘤坏死因子-α(TNF-α)对根尖乳头干细胞(SCAP)外增殖及成牙成骨向分化能力的影响。方法:采用酶消化法结合组织块法获得根尖乳头干细胞,免疫荧光免疫组化法鉴定细胞来源,通过不同浓度的TNF-α进行干预后检测对SCAP增殖,矿化成牙成骨能力的影响。结果:MTT检测显示各浓度组TNF-α均能刺激SCAP的体外增殖,10、50 mg/L的TNF-α实验组可明显促进SCAP增殖(P<0.05),差异具有统计学意义;茜素红染色显示与对照组(0 mg/L TNF-α)相比,实验组(5、10、50 mg/L TNF-α)染色明显变浅,矿化结节形成的较小,对照组形成红色结节范围大且呈深橘色;免疫组化染色显示SCAP胞浆中DSP、BSP均有表达,实验组部分细胞胞浆染色且染色较浅,呈褐色为阳性表达,对照组(0 mg/L TNF-α)细胞胞浆完全着色呈深褐色,为强阳性表达。结论:不同浓度TNF-α对SCAP的生长增殖均有促进作用,TNF-α在不同程度上对SCAP矿化及成牙成骨向分化有抑制作用。

关键词: 根尖乳头干细胞, 肿瘤坏死因子-α, 增殖, 矿化

Abstract: Objective: To investigate the effects of TNF-α on the proliferation and odontoblastic and osteogenic differentiation of stem cells from the apical papilla (SCAP) in vitro. Methods: Stem cells were isolated from the apical papillary by combining enzyme digestion with tissue block method, and identified by immunofluorescence and immunocytochemical method. After treated by TNF-α, the effect on the proliferation and mineralized tooth's ontogenesis ability were detected in SCAP. Results: TNF-α in each density group can stimulate the proliferation of SCAP in vitro, particularly in 10mg/L and 50mg/L (P<0.05). Additionally, in the experimental group, the Alizarin red staining was obvious weaker with smaller mineral node, and the expression of DSP and BSP were down-regulated compared to the control group. Conclusion: TNF-α could promote the proliferation and inhibit the mineralization of SCAP.

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