银杏叶提取物诱导骨髓间充质干细胞成骨分化过程中P38信号通路的研究

doi:10.13701/j.cnki.kqyxyj.2016.09.005

口腔医学研究 ›› 2016, Vol. 32 ›› Issue (9): 912-915.DOI: 10.13701/j.cnki.kqyxyj.2016.09.005

银杏叶提取物诱导骨髓间充质干细胞成骨分化过程中P38信号通路的研究

时舒曼¹,袁浩天²,张晓晓¹,吴哲^1,3*

1. 吉林大学口腔医学院修复科吉林长春 130021;
2. 中国人民解放军208医院吉林长春 130000;
3. 广州医科大学附属口腔医院广东广州 510500

收稿日期:2016-01-07 出版日期:2016-09-26 发布日期:2016-09-26
通讯作者: 吴哲,E-mail:1378725107@qq.com
作者简介:时舒曼(1991~ ),女,河南省许昌市人,硕士,主要从事口腔修复学研究。
基金资助:
吉林省产业技术研究与开发项目(编号:2013C023-5)

Ginkgo Biloba Extract Promotes Osteogenic Differentiation of Rat Bone Marrow Mesenchymal Stem Cells in a Pathway Involving P38 MAPK Signaling Pathway

SHI Shu-man¹, YUAN Hao-tian², ZHANG Xiao-xiao¹, WU Zhe^1,3*

1. Department of Prosthodontics, School of Stomatology, Jilin University, Changchun 130021, China;
2. Chinese PLA 208 Hospital, Changchun130000, China;
3. Stomatology School of Guangzhou Medical University, Guangzhou 510500, China

Received:2016-01-07 Online:2016-09-26 Published:2016-09-26

摘要/Abstract

摘要： 目的:探讨银杏叶提取物(ginkgo biloba extract,GBE)促进大鼠骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs)成骨分化的机制。方法:将150 mg/L GBE作用于第3代大鼠BMSCs,抑制组使用P38丝裂原活化蛋白激酶(mitogen-activated protein kinase,MAPK)信号通路抑制剂SB203580预处理,通过茜素红(alizarin red S,ARS)染色、碱性磷酸酶(Alkalinet phosphatase,ALP)法以及RT-PCR等检测方法判定GBE是否通过上调P38信号通路促进大鼠BMSCs成骨分化。结果:与实验对照组大鼠相比,抑制组的大鼠BMSCs细胞外基质矿化减少,ALP表达明显下降,成骨相关基因ALP、BMP-2、OCN、Runx2(runt-related transcription factor2)的表达量亦明显降低。结论:GBE通过上调P38信号通路促进大鼠BMSCs成骨分化。

关键词: 银杏叶提取物, 骨髓间充质干细胞, P38MAPK信号通路, 成骨分化

Abstract: Objective: To reveal the mechanism of GBE promoting osteogenic differentiation of rat BMSCs. Methods: 150mg/L GBE was applied to the third generation of rat BMSCs, while the inhibitor group underwent a pretreatment of SB203580, which was the specific inhibitor of P38 MAPK signaling pathway. The results were evaluated by ARS dye, ALP activity assay and RT-PCR. Results: Statistically significant difference was found between the control group and the inhibitor group. In the inhibitor group, extracellular calcium deposition, ALP activity and the expression of osteogenic-related genes including ALP, BMP-2, OCN and Runx2 were lower than the control group. Conclusion: GBE promoted osteogenic differentiation of rat BMSCs by upregulating the P38 MAPK signaling pathway.

Key words: Ginkgo biloba extract, Bone marrow mesenchymal stem cells, P38 MAPK signaling pathway, Osteogenic differentiation

中图分类号:

X703.5

时舒曼,袁浩天,张晓晓,吴哲. 银杏叶提取物诱导骨髓间充质干细胞成骨分化过程中P38信号通路的研究[J]. 口腔医学研究, 2016, 32(9): 912-915.

SHI Shu-man , YUAN Hao-tian , ZHANG Xiao-xiao , WU Zhe. Ginkgo Biloba Extract Promotes Osteogenic Differentiation of Rat Bone Marrow Mesenchymal Stem Cells in a Pathway Involving P38 MAPK Signaling Pathway[J]. Journal of Oral Science Research, 2016, 32(9): 912-915.

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银杏叶提取物诱导骨髓间充质干细胞成骨分化过程中P38信号通路的研究

Ginkgo Biloba Extract Promotes Osteogenic Differentiation of Rat Bone Marrow Mesenchymal Stem Cells in a Pathway Involving P38 MAPK Signaling Pathway

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