rhTNFR:Fc对兔正畸微种植体周围炎骨结合影响的研究

doi:10.13701/j.cnki.kqyxyj.2016.09.006

口腔医学研究 ›› 2016, Vol. 32 ›› Issue (9): 916-919.DOI: 10.13701/j.cnki.kqyxyj.2016.09.006

rhTNFR:Fc对兔正畸微种植体周围炎骨结合影响的研究

马国武¹,郑胜^2*

1. 苏州大学附属第二医院口腔科江苏苏州 215004;
2. 浙江中医药大学口腔医学院浙江杭州 310053

收稿日期:2016-03-22 出版日期:2016-09-26 发布日期:2016-09-26
通讯作者: 郑胜,E-mail:daniel_3@163.com
作者简介:马国武(1980~ ),男,山东人,硕士在读,主治医师,主要从事口腔科的临床治疗工作。
基金资助:
浙江省医药卫生科技项目(编号:2013KYB179)

Effect of rhTNFR:Fc on the Osseointegration of Peri-implantitis in Rabbit Model

MA Guo-wu¹, ZHENG Sheng²

1. The Second Affiliated Hospital of Suzhou University, Suzhou 215004, China;
2. Zhejiang Chinese Medical University, Hangzhou 310053, China

Received:2016-03-22 Online:2016-09-26 Published:2016-09-26

摘要/Abstract

摘要： 目的:评价注射用重组人Ⅱ型TNF受体-抗体融合蛋白(tumour necrosis factor FC fusion protein,rhTNFR:Fc)(商品名:益赛普)对兔正畸微种植体周围炎骨结合的影响。方法:选用新西兰兔12只,随机分为模型对照组和治疗组。分别在兔下颌无牙区植入正畸微种植体,采用钢丝结扎法建立实验性微种植体周围炎模型,结扎56 d,治疗组给予rhTNFR:Fc(剂量1.1 mg/kg·d^-1)1周,模型对照组给予等量生理盐水,两组在造模后第63天,行免疫组化染色来比较种植体周围TNF-α表达情况;CBCT技术及McNeal's染色法对种植体周围炎周围骨吸收及骨修复状态进行观察。结果:造模后63 d,治疗组TNF-α较对照组减少(P<0.05),影像学检查及McNeal's染色结果显示治疗组骨吸收程度轻于对照组。结论:rhTNFR:Fc可影响微种植体周围炎骨结合过程。

关键词: 重组人Ⅱ型TNF受体-抗体融合蛋白, 微种植体周围炎, 肿瘤坏死因子-α, 骨吸收, 骨修复

Abstract: Objective: To evaluate the effect of recombinant human tumour necrosis factor FC fusion protein (rhTNFR:Fc) on the osseointegration of non-surgical treatment peri-implantitis in a rabbit model. Methods: Twelve male adult rabbits were randomly chosen and divided into experimental group and control group. Peri-implantitis of micro implant in rabbit model was induced by securing silk ligatures around the implants in mandibular toothless alveolar for 8 weeks. Experimental group received rhTNFR:Fc (1.1mg/kg daily for 1 week) and control group received saline. Immunohistochemistry was applied to measure the TNF-α level after the application of rhTNFR:Fc; Cone beam CT and McNeal's staining were applied to evaluate the bone resorption and bone repair at the Implant-Bone interface. Results: Compared to control group, TNF-α in experimental group was significantly lower (P<0.05); Bone resorption was significantly decreased after application of rhTNFR:Fc. Conclusion: rhTNFR:Fc reduced tumour necrosis factor level and might contribute to the osseointegration of peri-implantitis.

Key words: rhTNFR:Fc, Peri-implantitis, Tumor necrosis factor-α, Bone resorption, Bone repair

中图分类号:

R781.4

马国武,郑胜. rhTNFR:Fc对兔正畸微种植体周围炎骨结合影响的研究[J]. 口腔医学研究, 2016, 32(9): 916-919.

MA Guo-wu , ZHENG Sheng. Effect of rhTNFR:Fc on the Osseointegration of Peri-implantitis in Rabbit Model[J]. Journal of Oral Science Research, 2016, 32(9): 916-919.

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rhTNFR:Fc对兔正畸微种植体周围炎骨结合影响的研究

Effect of rhTNFR:Fc on the Osseointegration of Peri-implantitis in Rabbit Model

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