口腔医学研究

口腔医学研究 ›› 2017, Vol. 33 ›› Issue (8): 803-806.DOI: 10.13701/j.cnki.kqyxyj.2017.08.001

• 名家论道 •    下一篇

血链球菌细菌素对牙龈卟啉单胞菌形貌及力学性质的影响

马晟利1*, 于舒扬2, 陈春晓2, 张金超2, 宋昕蔓2, 韩烨2   

  1. 马晟利1* 于舒扬2 陈春晓2 张金超2 宋昕蔓2 韩烨2
  • 收稿日期:2017-03-18 出版日期:2017-08-20 发布日期:2017-08-28
  • 通讯作者: 马晟利,E-mail:MSL6377@163.com
  • 作者简介:马晟利(1963~ ),女,黑龙江哈尔滨人,主任医师,硕士,主要从事口腔微生态与牙周病、黏膜病的研究工作。
  • 基金资助:
    黑龙江省自然科学基金(编号:H2015086)

Effect of Streptococcus Sanguis Bacteriocin on Morphology and Mechanical Properties of Porphyromonas Gingivalis.

MA Sheng-li1*, YU Shu-yang2, CHEN Chun-xiao2, ZHANG Jin-chao2, SONG Xin-man2, HAN Ye2   

  1. 1. Hospital of Heilongjiang Province, Harbin 150036, China;
    2. School of Stomatology, Jiamushi University, Jiamusi 154007, China.
  • Received:2017-03-18 Online:2017-08-20 Published:2017-08-28

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摘要： 目的:研究血链球菌细菌素(简称血链素)对牙龈卟啉单胞菌(Porphyromonas gingivalis,P.g)形貌特征及力学性质的影响。方法:1)复苏、传代并鉴定血链球菌标准菌株ATCC10556、牙龈卟啉单胞菌标准菌株ATCC33277。2)将血链球菌低温高速离心、细胞破碎等方法提取血链素,使之作用于P.g,于37 ℃厌氧培养48 h。3)采用原子力显微镜(AFM)的接触模式,观察血链素作用前后P.g菌体的形貌变化。4)利用AFM测定血链素作用前后P.g的力-距离曲线,并计算黏附力及杨氏模量。结果:1)通过AFM的观察:P.g在血链素的作用下,菌体直径缩小、平均粗糙度及平均峰高度增加(P<0.05)。2)通过AFM的观察:血链素作用后,P.g的黏附力、杨氏模量显著降低(P<0.001)。正常P.g的黏附力平均值为(0.70±0.10) nN、杨氏模量平均值为(5.54±0.16) MPa;经血链素作用后P.g的黏附力平均值为(0.50±0.10) nN、杨氏模量平均值为(3.97±0.64) MPa。结论:1)血链素可引起牙龈卟啉单胞菌形貌特征发生改变。2)血链素可导致牙龈卟啉单胞菌的黏附力及杨氏模量降低。

关键词: 血链球菌细菌素, 牙龈卟啉单胞菌, 原子力显微镜, 形貌特征, 力学性质

Abstract: Objective: To study the effects of streptococcus sanguis bacteriocins on the surface morphology and the mechanical properties of porphyromonas gingivalis (P.g). Methods: Standard strains ATCC10556 and ATCC33277 were recovered and extended. Streptococcus sanguis bacteriocins was extracted by low temperature high-speed centrifugation and ultrasonic broken. Streptococcus sanguis bacteriocins was affected with P.g at 37℃ and anaerobic cultured for 48h. Atomic force microscope (AFM) was used to observe the microstructure of Streptococcus sanguis bacteriocins on P.g. Through the force-distance curve, the Young's modulus and adhesion of Streptococcus sanguis bacteriocins on P.g were calculated. Results: AFM showed that Streptococcus sanguis bacteriocins could reduce the diameter of P.g, rise the average roughness and average peak height (P<0.05). After Streptococcus sanguis bacteriocins on P.g, the adhesion and the Young's modulus of P.g reduced significantly (P<0.001). The adhesion and the Young's modulus of P.g were (0.7±0.1)nN and (5.54±0.16)Mpa. After Streptococcus sanguis bacteriocins on P.g, the adhesion and the Young's modulus of P.g were 0.50± 0.1nN and 3.97±0.64Mpa. Conclusion: Streptococcus sanguis bacteriocins can change the morphology of P.g and reduce the adhesion and the Young's modulus of P.g.

Key words: Streptococcus sanguis bacteriocins , Porphyromonas gingivalis Atomic force microscope (AFM) , Morphology , Mechanical properties

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