碳酸酐酶Ⅵ与4～5岁儿童龋病相关性的研究

doi:10.13701/j.cnki.kqyxyj.2018.04.007

口腔医学研究 ›› 2018, Vol. 34 ›› Issue (4): 363-366.DOI: 10.13701/j.cnki.kqyxyj.2018.04.007

碳酸酐酶Ⅵ与4～5岁儿童龋病相关性的研究

侯雯¹, 苏达², 阙国鹰^3*

1. 首都医科大学附属北京地坛医院口腔科北京 100015;
2. 厦门市口腔医院福建厦门 361000;
3. 南方医科大学口腔医院·广东省口腔医院广东广州 510260

收稿日期:2017-09-14 出版日期:2018-04-28 发布日期:2018-04-25
通讯作者: 阙国鹰,E-mail:2917268593@qq.com
作者简介:侯雯(1982~),女,湖南人,主治医师,硕士,主要从事口腔内科的临床治疗工作。

Correlation between Carbonic Anhydrase Ⅵ Level and Dental Caries among Children Aged 4-5 Years.

HOU Wen¹, SU Da², QUE Guo-ying^3*

1. Department of Stomatology, Beijing Ditan Hospital Capital Medical University, Beijing 100015, China;
2. Xiamen Stomatological Hospital, Xiamen 361000, China;
3. Stomatological Hospital of Southern Medical University, Guangdong Provincial Stomatology Hospital, Guangzhou 510260, China.

Received:2017-09-14 Online:2018-04-28 Published:2018-04-25

摘要/Abstract

摘要： 目的:初步探讨4～5岁儿童唾液、菌斑液中碳酸酐酶Ⅵ(CA-Ⅵ)含量与龋病的关系。方法:以无龋组、低龋组、高龋组儿童各33名为研究对象,采用ELISA法检测唾液、菌斑液中CA-Ⅵ含量,H+-ISFET测定菌斑液pH值,并进行统计学分析。结果:唾液CA-Ⅵ含量:无龋组＞低龋组＞高龋组,各组间有统计学差异(P<0.05);菌斑液CA-Ⅵ含量:无龋组＞低龋组＞高龋组,各组间有统计学差异(P<0.05);唾液、菌斑液CA-Ⅵ含量和菌斑液pH值呈线性正相关(P<0.001)。结论:随着龋病严重程度的增加,唾液、菌斑液CA-Ⅵ水平呈下降趋势。CA-Ⅵ可上调牙表面菌斑pH值,对保护牙齿、抵抗龋病有重要作用。

关键词: 碳酸酐酶Ⅵ, 龋病, 菌斑, 唾液

Abstract: Objective: To investigate the relationship between dental caries and CA-Ⅵ levels in saliva and dental plaque fluid in children aged 4-5 years. Methods: Thirty-three children were randomly selected for each group: caries-free group, low caries group, and high caries group. The levels of CA-Ⅵ in dental plaque and saliva were examined by ELISA. The pH values of dental plaque fluid were detected by H+-ISFET. The results were statistically analyzed. Results: The CA-Ⅵ levels in saliva were statistically different among three groups: caries-free group>low caries group>high caries group (P<0.05). The CA-Ⅵ levels in dental plaque fluid were significantly different among three groups: caries-free group>low caries group>high caries group (P<0.05). Pearson's correlation analysis showed CA-Ⅵ levels in saliva and dental plaque fluid were positively related to pH values of dental plaque fluid (P<0.001). Conclusion: With the increase of caries severity, the levels of CA-Ⅵ in saliva and dental plaque fluid decreased. CA-Ⅵ might increase the pH values of plaque on the dental surface, and play an important role in tooth protection and caries resistance.

Key words: Carbonic Anhydrase Ⅵ, Dental caries, Dental plaque, Saliva

侯雯, 苏达, 阙国鹰. 碳酸酐酶Ⅵ与4～5岁儿童龋病相关性的研究[J]. 口腔医学研究, 2018, 34(4): 363-366.

HOU Wen, SU Da, QUE Guo-ying. Correlation between Carbonic Anhydrase Ⅵ Level and Dental Caries among Children Aged 4-5 Years.[J]. Journal of Oral Science Research, 2018, 34(4): 363-366.

参考文献

[1] Mahjoub S, Ghasempour M, Gharage A, et al. Comparison of total antioxidant capacity in saliva of children with severe early childhood cariesand caries-free children [J]. Caries Res, 2014, 48(4)∶271-275
[2] Stookey GK. The effect of saliva on dental caries [J]. J Am Dent Assoc, 2008, 139(5)∶11-17
[3] McKenna R, Frost SC. Overview of the carbonic anhydrase family [J]. Subcell Biochem, 2014, 75(1)∶3-5
[4] Kivela J, Parkkila S, Waheed A, et al. Secretory carbonic anhydrase isoenzyme (CA-Ⅵ) in human serum [J]. Clin Chem, 1997, 43(12)∶2318-2322
[5] Parkkila S, Parkkila AK, Kaunisto K, et al. Location of a membrane-bound carbonic anhydrase isoenzyme (CA-Ⅳ) in the human male reproductive tract [J]. J Histochem Cytochem, 1993, 41(5)∶751-757
[6] Parkkila S, Parkkila AK, Rajaniemi H. Circadian periodicity in salivary carbonic anhydrase Ⅵ concentration [J]. Acta Physiol Scand, 1995, 154(2)∶205-211
[7] Parkkila S, Kaunisto K, Rajaniemi L, et al. Immunohistochemical localization of carbonic anhydrase isoenzymes Ⅵ, Ⅱ, and I in human parotid andsubmandibular glands [J]. J Histochem Cytochem, 1990, 38(7) ∶941-947
[8] Kivela J, Parkkila S, Parkkila AK, et al.A low concentration of carbonic anhydrase isoenzyme Ⅵ in whole saliva is associated with caries prevalence [J]. Caries Res, 1999, 33(3)∶178-184
[9] 王俭,郑树国,王晓灵.乳牙菌斑液pH值与患龋状况及摄糖频率的关系[J].现代口腔医学杂志,2006,20(6)∶643-645
[10] Dodds MW, Johnson DA, Mobley CC, et al. Parotid saliva protein profiles in caries-free and caries-active adults [J]. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 1997, 83(2)∶244-251
[11] Navazesh M. How can oral health care providers determine if patients have dry mouth [J]. J Am Dent Assoc, 2003, 134(5)∶613-618
[12] Leinonen J, KivelA J, Parkkila S, et al. Salivary carbonic anhIydrase isoanzyme Ⅵ is located in the human enamel pellicle [J]. Caries Res, 1999, 33(3)∶185
[13] Leinonen JS, Saari KA, Seppanen JM, et al. Immunohistochemical demonstration of carbonic anhydrase isoenzyme Ⅵ (CA-Ⅵ) expression in rat lower airways and lung [J]. J Histochem Cytochem, 2004, 52(8) ∶1107-1112
[14] Kimoto M, Kishino M, Yura Y, et al. A role of salivary carbonic anhydrase Ⅵ in dental plaque [J]. Arch Oral Biol, 2006, 51(2)∶117-122
[15] Li ZQ, Hu XP, Zhou JY, et al. Genetic polymorphisms in the carbonic anhydrase Ⅵ gene and dental caries susceptibility [J]. Genet Mol Res, 2015, 14(2)∶5986-5993
[16] Yildiz G, Ermis RB, Calapoglu NS, et al. Gene-environment interactions in the etiology of dental caries [J]. Dent Res, 2016, 95(1)∶74-79
[17] Sengul F, Kilic M, Gurbuz T, et al. Carbonic anhydrase Ⅵ gene polymorphism rs2274327 relationship between salivary parameters and dental-oral health status in children [J]. Biochem Genet, 2016, 54(4)∶467-475
[18] 余占海,胡晓潘,李志强,等.碳酸酐酶-6基因多态性与龋病易感性的初步研究[J].口腔医学研究,2013,29(2)∶167-170

碳酸酐酶Ⅵ与4～5岁儿童龋病相关性的研究

Correlation between Carbonic Anhydrase Ⅵ Level and Dental Caries among Children Aged 4-5 Years.

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