MMP-9、COX-2、NF-κB在口腔鳞状细胞癌组织中的表达及意义

doi:10.13701/j.cnki.kqyxyj.2021.04.018

摘要/Abstract

摘要： 目的: 探讨基质金属蛋白酶-9(MMP-9)、环氧化物酶-2(COX-2)、细胞核因子KB(NF-κB)在口腔鳞状细胞癌(OSCC)组织中的表达及意义。方法: 分别采用实时荧光定量聚合酶链反应(qRT-PCR)、蛋白免疫印迹法(Western blot)、免疫组化法检测80例OSCC组织中的表达水平;采用Pearson法分析OSCC组织中MMP-9、COX-2、NF-κB表达的相关性;OSCC患者进行3年随访,采用Kaplan-Meier对生存情况进行分析,Log-Rank法检测组间生存差异;COX回归分析影响患者预后的危险因素。结果: 与正常组比较,OSCC组织中MMP-9、COX-2、NF-κB mRNA及蛋白水平显著升高(P＜0.05);与正常组相比,OSCC组MMP-9、COX-2、NF-κB蛋白阳性表达增强(P＜0.05);MMP-9与COX-2呈正相关(r=0.356,P=0.012),MMP-9与NF-κB呈正相关(r=0.312,P=0.005),COX-2与NF-κB呈正相关(r=0.619,P=0.000);MMP-9、COX-2、NF-κB表达与OSCC患者病理分级、分化程度、淋巴结转移及TNM分期密切相关(P＜0.05);MMP-9阴性表达组总体生存率高于阳性表达组(P＜0.05),COX-2阴性表达组总体生存率高于阳性表达组(P＜0.05),NF-κB阴性表达组总体生存率高于阳性表达组(P＜0.05);COX回归分析显示淋巴结转移、TNM分期、MMP-9、COX-2、NF-κB可能是影响OSCC患者预后的独立危险因素(P＜0.05)。结论: MMP-9、COX-2、NF-κB在口腔鳞状细胞癌组织中呈高表达,其表达量与患者临床病理参数密切相关。

关键词: 基质金属蛋白酶-9, 环氧化物酶-2, 细胞核因子κB, 口腔鳞状细胞癌, 预后

Abstract: Objective: To explore the expression and significance of MMP-9, COX-2, and NF-κB in oral squamous cell carcinoma (OSCC) tissues. Methods: qRT-PCR, western blot, and immunohistochemistry were used to detect the expression levels in 80 cases of OSCC tissues. Correlation of MMP-9, COX-2, and NF-κB in OSCC tissues was analyzed using the Pearson method. OSCC patients were followed for 3 years, Kaplan-Meier was used to analyze the survival, and Log-Rank method was used to detect the survival differences between groups. COX regression analysis was used to detect the risk factors affecting the prognosis of patients. Results: Compared with the normal group, the levels of MMP-9, COX-2, NF-κB mRNA and protein in OSCC tissues were significantly increased (P<0.05). Compared with the normal group, the positive expressions of MMP-9, COX-2, and NF-κB proteins in the OSCC group were enhanced (P<0.05). MMP-9 was positively correlated with COX-2 (r=0.356, P=0.012) and NF-κB (r=0.312, P=0.005), and COX-2 was positively correlated with NF-κB (r=0.619, P=0.000). MMP-9, COX-2, and NF-κB were closely related to the pathological grade, degree of differentiation, lymph node metastasis, and TNM stage in OSCC patients (P<0.05). The overall survival rate of the MMP-9 negative expression group was higher than that of the positive expression group (P<0.05). The overall survival rate of the COX-2 negative expression group was higher than that of the positive expression group (P<0.05), and the overall survival rate of the NF-κB negative expression group was higher in the positive expression group (P<0.05). COX regression analysis showed that lymph node metastasis, TNM staging, MMP-9, COX-2, and NF-κB may be independent risk factors affecting the prognosis of OSCC patients (P<0.05). Conclusion: MMP-9, COX-2, and NF-κB were highly expressed in OSCC tissues. The expression levels of MMP-9, COX-2, and NF-κB were closely related to the clinicopathological parameters of patients.

Key words: matrix metalloproteinases-9, cyclooxygenase 2, nuclear factor kappa gene binding, oral squamous cell carcinoma, prognosis

张旭, 刘佳文. MMP-9、COX-2、NF-κB在口腔鳞状细胞癌组织中的表达及意义[J]. 口腔医学研究, 2021, 37(4): 360-365.

ZHANG Xu, LIU Jiawen. Expression and Significance of MMP-9, COX-2, and NF-κB in Oral Squamous Cell Carcinoma[J]. Journal of Oral Science Research, 2021, 37(4): 360-365.

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