仿生微/纳米多级界面增强植入体骨整合的体内研究

doi:10.13701/j.cnki.kqyxyj.2021.04.019

口腔医学研究 ›› 2021, Vol. 37 ›› Issue (4): 366-370.DOI: 10.13701/j.cnki.kqyxyj.2021.04.019

仿生微/纳米多级界面增强植入体骨整合的体内研究

时子文, 祝颂松, 姜楠^*

口腔疾病研究国家重点实验室国家口腔疾病临床医学研究中心四川大学华西口腔医院正颌及关节外科四川成都 610041

收稿日期:2020-09-21 发布日期:2021-04-15
通讯作者: *姜楠,E-mail:dent_jn@163.com
作者简介:时子文(1995~ ),女,山东烟台人,硕士在读,主要从事口腔正颌及关节外科。
基金资助:
国家自然科学基金(编号:81901026)

Development of Biomimetic Micro/Nano Hierarchical Interface for Enhancement of Osseointegration

SHI Ziwen, ZHU Songsong, JIANG Nan^*

State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Department of Orthognathic and Temporomandibular Joint Surgery, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China

Received:2020-09-21 Published:2021-04-15

摘要/Abstract

摘要： 目的: 研究一种仿生微/纳米多级界面以增强植入体骨整合。方法: 纯钛样本分别经放电等离子烧结、电化学阳极氧化或二者复合处理后,分别形成微米级骨小梁仿生结构、TiO₂纳米管表面改性结构、微米级骨小梁仿生/TiO₂纳米管表面改性结构。取40只雌性SD大鼠随机分为4组(A、B、C、D,n=10),在其膝关节内侧建立直径为1.5 mm的骨缺损模型,分别植入未处理钛、微米级骨小梁仿生钛、TiO₂纳米管表面改性钛、微米级骨小梁仿生/TiO₂纳米管表面改性钛。12周后,对近端胫骨及钛植入体行组织学分析、Micro-CT评价及生物力学测试。结果: D组骨整合率,相对骨体积和骨体积最高,多项骨小梁相关参数较高,表现出更好的骨整合。此外,D组的最大推出力和临界抗剪切强度均高于其他3组(P<0.05)。结论: 仿生微/纳米多级界面增强了钛植入体的骨整合,可作为一种性能优良的材料用于临床治疗。

关键词: 微/纳米多级界面, 骨整合, 植入体, TiO₂纳米管, 复合处理

Abstract: Objective: To investigate a biomimetic micro/nano hierarchical interface to enhance osseointegration. Methods: The pure titanium samples were modified by three surface treatment methods, namely spark plasma sintering, electrochemical anodization, and composite treatment, which formed Micro-Ti, Nano-Ti and Micro/Nano-Ti structure, respectively. Forty female SD rats were randomly assigned into four groups (Group A, B, C, and D, n=10 animals per group). Bone defects in the diameter of 1.5mm were established on the medial side of the knee joint and inserted with untreated-Ti, Micro-Ti, Nano-Ti, and Micro/Nano-Ti, respectively. Twelve weeks following implantation, the rats were sacrificed and the proximal tibiaes (together with the titanium implants) were harvested for histological analysis, Micro-CT evaluation, and biomechanical testing. Results: Group D had the highest percent of osseointegration, percent bone volume, and bone volume. Multiple relative parameters of trabeculae of group D were relatively high. The compression force and ultimate shear strength of group D were significantly higher than those of the other three groups (P<0.05). Conclusion: Biomimetic micro/nano hierarchical interface enhanced the osseointegration of titanium implants.

Key words: micro/nano hierarchical interface, osseointegration, implants, titania nanotubes, composite treatment

时子文, 祝颂松, 姜楠. 仿生微/纳米多级界面增强植入体骨整合的体内研究[J]. 口腔医学研究, 2021, 37(4): 366-370.

SHI Ziwen, ZHU Songsong, JIANG Nan. Development of Biomimetic Micro/Nano Hierarchical Interface for Enhancement of Osseointegration[J]. Journal of Oral Science Research, 2021, 37(4): 366-370.

参考文献

[1] He Y, Mu C, Shen X, et al. Peptide LL-37 coating on microstructured titanium implants to facilitate bone formation in vivo via mesenchymal stem cell recruitment [J]. Acta Biomaterialia, 2018, 80(10): 412.
[2] Ferraris S, Cochis A, Cazzola M, et al. Cytocompatible and anti-bacterial adhesion nanotextured Titanium Oxide layer on Titanium surfaces for dental and orthopedic implants [J]. Front Bioeng Biotechnol,2019,103(7):103.
[3] Azzawi ZGM, Hamad TI, Kadhim SA, et al. Osseointegration evaluation of laser-deposited titanium dioxide nanoparticles on commercially pure titanium dental implants [J]. J Mater Sci Mater Med, 2018, 29(7): 96.
[4] Lin K, Xia L, Gan J, et al. Tailoring the nanostructured surfaces of hydroxyapatite bioceramics to promote protein adsorption, osteoblast growth, and osteogenic differentiation [J]. ACS Appl Mater Interfaces, 2013, 5(16): 8008-8017.
[5] 杨帮成,周学东,于海洋,等.钛植入体表面改性方法[J].华西口腔医学杂志, 2019, 37(2): 124-129.
[6] Dundar S, Yaman F, Bozoglan A, et al. Comparison of osseointegration of five different surfaced titanium implants [J]. J Craniofac Surg, 2018, 29(7): 1991-1995.
[7] Pinotti FE, de Oliveira GJPL, Aroni MAT, et al. Analysis of osseointegration of implants with hydrophilic surfaces in grafted areas:a preclinical study [J]. Clin Oral Implants Res, 2018, 29(10): 963-972.
[8] Li L, Li M, Li D, et al. Chemical functionalization of bone implants with nanoparticle-stabilized chitosan and methotrexate for inhibiting both osteoclastoma formation and bacterial infection [J]. J Mater Chem B, 2014, 2(36): 5952-5961.
[9] Vahabzadeh S, Roy M, Bandyopadhyay A, et al. Phase stability and biological property evaluation of plasma sprayed hydroxyapatite coatings for orthopedic and dental applications [J]. Acta Biomater, 2015, 17: 47-55.
[10] Kuwabara A, Hori N, Sawada T, et al. Enhanced biological responses of a hydroxyapatite/TiO₂ hybrid structure when surface electric charge is controlled using radiofrequency sputtering [J]. Dent Mater J, 2012, 31(3): 368-376.
[11] Camps-Font O,Martin-Fatas P,Cle-Ovejero A, et al. Postoperative infections after dental implant placement: Variables associated with increased risk of failure [J]. J Periodontol, 2018, 89(10) :1165-1173.
[12] 蔡彦坤,郑国莹,隋磊.纯钛或钛合金种植材料表面不同纳米结构对细胞行为的影响[J].口腔医学研究,2018,34(7):699-702.
[13] Andrukhov O, Huber R, Shi B, et al. Proliferation, behavior, and differentiation of osteoblasts on surfaces of different microroughness [J]. Dent Mater, 2016, 32(11):1374-1384.
[14] Garrett PW, Johnston GW, Bosshardt DD, et al. Hard and soft tissue evaluation of titanium dental implants and abutments with nanotubes in canines [J]. J Periodontol, 2020, 91(4):516-523.
[15] Zhao L, Mei S, Chu PK, et al. The influence of hierarchial hybrid micro/nano-textured titanium surface with titania nanotubes on osteoblast functions [J]. Biomaterials, 2010, 31(19)∶5072-5082.
[16] Souza JCM, Sordi MB, Kanazawa M, et al. Nano-scale modification of titanium implant surfaces to enhance osseointegration [J]. Acta Biomater, 2019, 94:112-131.
[17] Liu F, Li Y, Liang J, et al. Effects of micro/nano strontium-loaded surface implants on osseointegration in ovariectomized sheep [J]. Clin Implant Dent Relat Res, 2019, 21(2):377-385.
[18] Jemat A, Ghazali MJ, Razali M, et al. Surface modifications and their effects on titanium dental implants [J]. Biomed Res Int, 2015, 2015: 791725.

仿生微/纳米多级界面增强植入体骨整合的体内研究

Development of Biomimetic Micro/Nano Hierarchical Interface for Enhancement of Osseointegration

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