口腔医学研究

口腔医学研究 ›› 2025, Vol. 41 ›› Issue (9): 761-766.DOI: 10.13701/j.cnki.kqyxyj.2025.09.005

• 龋病牙髓病学研究 • 上一篇    下一篇

臭氧水对口腔主要致龋菌的体外抑菌作用研究

王声远, 姜瑞, 施华肃, 刘跃芳, 罗佳露, 徐燕*   

  1. 遵义医科大学医学与科技学院 贵州 遵义 563000
  • 收稿日期:2025-03-05 出版日期:2025-09-28 发布日期:2025-09-24
  • 通讯作者: * 徐燕,E-mail:496541772@qq.com
  • 作者简介:王声远(2003~ ),男,广州人,本科在读,研究方向:口腔医学教育、牙体牙髓病学、口腔生物学研究。
  • 基金资助:
    贵州省省级教学内容和课程体系改革项目(编号:2022389);贵州省大学生创新创业训练计划(编号:S2024136532503)

In vitro Bactericidal Effect of Ozonated Water on Major Oral Cariogenic Bacteria

WANG Shengyuan, JIANG Rui, SHI Huasu, LIU Yuefang, LUO Jialu, XU Yan*   

  1. Medicine and Technology College of Zunyi Medical University, Zunyi 563000, China
  • Received:2025-03-05 Online:2025-09-28 Published:2025-09-24

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摘要： 目的: 探究不同臭氧水浓度和作用时间对龋病主要致病菌的抑菌作用,明确其临床应用潜力。方法: 测定臭氧水对变形链球菌、乳酸杆菌和粘性放线菌的最佳抑菌浓度;用同一浓度臭氧水作用不同时间,确定最佳作用时间;以酸碱度变化和生物被膜形成量为结局指标判定臭氧水对致龋菌的繁殖影响;收集正常成人唾液,模拟口腔环境,测定唾液对臭氧水抑菌的影响。结果: 3种致龋菌的最佳臭氧水抑菌浓度在3.2 ppm,抑制变形链球菌的最佳作用时间为60 s,乳酸杆菌为120 s,粘性放线菌为60 s;3种致龋菌在臭氧水作用下产酸能力降低15.1%,生物被膜形成能力也有所下降,但臭氧水混合唾液后抑菌率下降至65.2%。结论: 臭氧水在安全浓度下具有高效抑菌作用,为龋病防治提供新策略。

关键词: 臭氧水, 变形链球菌, 口腔致龋菌, 抑菌作用, 粘性放线菌, 唾液, 生物被膜, 龋病

Abstract: Objective: To investigate the antibacterial effects of ozonated water at varying concentrations and exposure durations against major cariogenic pathogens. Methods: The optimal antibacterial concentration of ozonated water against Streptococcus mutans (S. mutans), Lactobacillus, and Actinomyces viscosus (A. viscosus) was determined. The same concentration was then applied for different durations to identify the optimal exposure time. Changes in pH and biofilm formation capacity were measured as outcome indicators to evaluate the inhibitory effects of ozonated water on bacterial proliferation. Saliva collected from healthy adults was used to simulate the oral environment and assess its influence on the antibacterial efficacy of ozonated water. Results: The optimal antibacterial concentration for all three cariogenic strains was 3.2 ppm. The most effective exposure durations were 60 seconds for S. mutans, 120 seconds for Lactobacillus, and 60 seconds for A. viscosus. Ozonated water reduced acid production by 15.1% and inhibited biofilm formation in all tested strains. However, when mixed with saliva, its antibacterial efficacy decreased to 65.2%. Conclusion: Ozonated water exhibits potent antibacterial effects at safe concentrations, demonstrating promising potential as a novel strategy for caries prevention and treatment.

Key words: ozonated water, Streptococcus mutans, cariogenic bacteria, antibacterial effect, Acvtinomyces viscosus, saliva, biofilms, dental caries