口腔医学研究

口腔医学研究 ›› 2025, Vol. 41 ›› Issue (12): 1055-1058.DOI: 10.13701/j.cnki.kqyxyj.2025.12.007

• 牙体牙髓病学研究 • 上一篇    下一篇

槲皮素对人牙本质抗酸蚀性能影响的原位研究

蒋能舞1,2, 黄玉霞2, 郑章龙2, 毕玮2,3*   

  1. 1.福建医科大学附属口腔医院修复科 福建 福州 350002;
    2.复旦大学附属中山医院厦门医院口腔科 福建 厦门 361015;
    3.复旦大学附属中山医院口腔科 上海 200032
  • 收稿日期:2025-03-21 出版日期:2025-12-28 发布日期:2025-12-23
  • 通讯作者: *毕玮,E-mail:biwei@zs-hospital.cn
  • 作者简介:蒋能舞(1991~ ),女,福建厦门人,硕士,主治医师,研究方向:口腔修复学。
  • 基金资助:
    福建省自然科学基金面上项目(编号:2022J011424)

Effects of Quercetin on Human Dentin Resistance to Erosion on in-situ Study

JIANG Nengwu1,2, HUANG Yuxia2, ZHENG Zhanglong2, BI Wei2,3*   

  1. 1. Department of Prosthodontics, Affiliated Stomatological Hospital, Fujian Medical University, Fuzhou 350002, China;
    2. Department of Stomatology, Xiamen Branch, Zhongshan Hospital, Fudan University, Xiamen 361015, China;
    3. Department of Stomatology, Zhongshan Hospital, Fudan University, Shanghai 200032, China
  • Received:2025-03-21 Online:2025-12-28 Published:2025-12-23

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摘要： 目的: 通过原位实验,探究槲皮素对人牙本质抗酸蚀性能的影响。方法: 制备20个牙本质块(2 mm×2 mm×2 mm),随机分为两组,一组采用1.23×104 μg/mL氟化钠,另一组使用300 μg/mL的槲皮素。通过循环体外酸蚀-原位再矿化,以模拟口腔环境。牙本质经对应溶液处理后,10名志愿者每日佩戴含牙本质试件的牙合垫式牙托,期间进行4次体外循环酸蚀处理:柠檬酸(pH=2.45)浸泡5 min×4次,实验为期7 d。对样品进行酸蚀前后的表面显微硬度(surface microhardness,SMH)及表面轮廓的检测,并计算表面显微硬度变化量(SMH%)及表面丧失量。结果: 所有志愿者均顺利完成实验流程。数据显示,槲皮素组的SMH%为(6.22±2.94)%,显著低于氟化钠组的(19.23±5.78)%(P<0.01)。槲皮素组的表面丧失量(0.97±0.34) μm也显著低于氟化钠组[(2.61±0.97) μm,(P<0.01]。结论: 在模拟日常口腔环境的条件下,短期(2 min)应用300 μg/mL槲皮素溶液能够显著提升牙本质抗酸蚀性能,其效果优于传统高浓度[(1.23×104) μg/mL]氟化钠溶液。

关键词: 槲皮素, 基质金属蛋白酶抑制剂, 牙酸蚀症, 牙本质

Abstract: Objective: To investigate the effect of quercetin on the human dentin resistance to erosion on in-situ study. Methods: Twenty dentin blocks (2 mm×2 mm×2 mm) were prepared and randomly divided into two groups, which were treated with 1.23×104 μg/mL sodium fluoride and 300 μg/mL quercetin, respectively. The oral environment was simulated through the in-vitro acid and in-situ remineralization cycle. After treated with corresponding solution, dentin specimens were wore by ten volunteers in the customized intraoral appliance. Four cycles of acid etching were performed during this period: soaked in citric acid (pH=2.45) for 4 times with 5 min in each time. The experiment was lasted for 7 days. The SMH and surface profiles were detected before and after erosion by using the surface microhardness tester and contact profilometry, respectively. The SMH% and the surface loss were calculated. Results: In the in situ experiment, all volunteers completed the experiment process. The SMH% of quercetin group was (6.22±2.94)%, which was significantly lower than that of sodium fluoride group [(19.23±5.78)%, P<0.01]. The surface loss in quercetin group [(0.97±0.34) μm] was significantly lower than that in sodium fluoride group [(2.61±0.97) μm , P<0.01]. Conclusion: Based on the in situ experiment, the short-term (2 min) application of 300 μg/mL quercetin solution can significantly improve the acid corrosion resistance of dentin, and the effect is better than the traditional high concentration [(1.23×104) μg/mL] sodium fluoride solution.

Key words: quercetin, matrix metalloproteinase inhibitor, dental erosion, dentin