小鼠牙胚发育过程中细胞骨架分子表达谱的变化和特征分析

doi:10.13701/j.cnki.kqyxyj.2026.02.012

口腔医学研究 ›› 2026, Vol. 42 ›› Issue (2): 151-156.DOI: 10.13701/j.cnki.kqyxyj.2026.02.012

小鼠牙胚发育过程中细胞骨架分子表达谱的变化和特征分析

贾慧^1,2, 冯笑梅², 张燕丽^2*, 段小红^2*

1.西北大学医学院陕西西安 710069;
2.口颌系统重建与再生全国重点实验室,国家口腔疾病临床医学研究中心,陕西省口腔医学重点实验室,空军军医大学口腔医院口腔生物学教研室,空军军医大学口腔医院口腔罕见病与遗传病门诊陕西西安 710032

收稿日期:2025-04-30 发布日期:2026-02-28
通讯作者: *张燕丽,E-mail: yanlizhang@fmmu.edu.cn; 段小红,E-mail: xhduan@fmmu.edu.cn
作者简介:贾慧(2001~ ),女,陕西人,硕士在读,研究方向:牙齿发育与遗传性疾病。
基金资助:
国家自然科学基金(项目编号:82370907); 空军军医大学“快速响应”科研课题(编号:2022KXKT004); 中国牙病防治基金会科研项目(编号:COHF-HJB202402)

Changes and Characteristics Analysis of Cytoskeleton Molecular Expression Profiles during Mouse Tooth Embryo Development

JIA Hui^1,2, FENG Xiaomei², ZHANG Yanli^2*, DUAN Xiaohong^2*

1. School of Medicine, Northwestern University, Xi'an, 710069, China;
2. State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, National Clinical Research Center for Oral Diseases, Shaanxi Key Laboratory of Stomatology, Department of Oral Biology, Clinic of Oral Rare Diseases and Genetic Diseases, School of Stomatology, The Fourth Military Medical University, Xi'an 710032, China

Received:2025-04-30 Published:2026-02-28

摘要/Abstract

摘要： 目的: 细胞骨架在牙胚发育中发挥关键作用,本研究通过整合转录组测序与生物信息学分析技术,探究小鼠牙胚不同发育时期细胞骨架相关分子的表达特征。方法: 采用出生后1.5 d的C57BL/6小鼠,体式显微镜下分离磨牙牙胚,提取总RNA进行转录组测序,并从GEO数据库(GSE255946)获取小鼠胚胎期14.5 d及16.5 d的牙胚转录组数据。比较牙胚发育在帽状期、钟状早期和钟状晚期时细胞骨架相关基因的表达变化,筛选差异表达基因。结果: 在牙胚的发育进程中,微丝、微管和中间丝3种骨架成分呈现动态表达变化。从帽状期至钟状早期15个基因表达显著上调,6个基因表达下调;从钟状早期至钟状晚期 3个基因表达上调,4个基因表达下调。结论: 细胞骨架相关分子在牙胚中呈现高丰度表达特征,其动态变化与牙胚生长发育进程展现出高度协同的表达模式,参与调控牙胚形态发生和细胞分化。未来研究重点可聚焦牙胚中每百万映射片段上的每千碱基转录本片段数(fragments per kilobase of transcript per million fragments mapped,FPKM)值较高且功能尚未阐明的分子。

关键词: 牙胚, 细胞骨架, 转录组测序, 生物信息学分析

Abstract: Objective: To characterize the expression of cytoskeleton-related molecules in mouse teeth germs. Methods: Transcriptome sequencing results of cytoskeleton-related molecules were collected from C57BL/6 mouse teeth germs at the cap, early bell, and late bell stages. The expression changes of these molecules were compared and screened for differentially expressed genes. Results: During the development of the teeth germs from the cap to the late bell stage, the genes related to the three cytoskeleton components showed dynamic expression changes. Fifteen genes were significantly up-regulated from the cap to the early bell stage and 6 genes were down-regulated. Three genes were up-regulated and 4 genes were down-regulated from the early to late bell stage. Conclusion: Cytoskeleton-associated molecules are abundant in teeth germs and show a highly coordinated expression pattern with the growth and development of teeth germs, which are involved in the regulation of morphogenesis and cell differentiation of teeth germs. Future research focuses can be placed on molecules in teeth germs with high FPKM values and whose functions have not yet been clarified.

Key words: teeth germ, cytoskeleton, transcriptome sequencing, bioinformatics analysis

贾慧, 冯笑梅, 张燕丽, 段小红. 小鼠牙胚发育过程中细胞骨架分子表达谱的变化和特征分析[J]. 口腔医学研究, 2026, 42(2): 151-156.

JIA Hui, FENG Xiaomei, ZHANG Yanli, DUAN Xiaohong. Changes and Characteristics Analysis of Cytoskeleton Molecular Expression Profiles during Mouse Tooth Embryo Development[J]. Journal of Oral Science Research, 2026, 42(2): 151-156.

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小鼠牙胚发育过程中细胞骨架分子表达谱的变化和特征分析

Changes and Characteristics Analysis of Cytoskeleton Molecular Expression Profiles during Mouse Tooth Embryo Development

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