大鼠种植体周围病IL-1β及IL-10与临床和影像学指标相关性研究

doi:10.13701/j.cnki.kqyxyj.2026.03.004

摘要/Abstract

摘要： 目的:基于体外炎症因子变化,构建大鼠种植体周围病(peri-implant disease,PID)模型,探究白细胞介素(interleukin,IL)-1β、IL-10与其临床和影像学指标相关性。方法:体外实验使用脂多糖和γ-干扰素刺激THP-1细胞模拟炎症微环境,荧光定量聚合酶链反应(real-time fluorescence quantitative PCR,RT-qPCR)检测IL-1β和IL-10 mRNA表达。动物实验将定制种植体植入20只SD大鼠上颌第一磨牙近中,4周后选12只骨整合良好大鼠,分为对照组与模型组(各6只)。模型组通过结扎及涂抹牙龈卟啉单胞菌(Porphyromonas gingivalis,P.g)悬浮液诱导PID,对照组不进行干预。每周记录种植体周围探诊深度(probing pocket depth,PPD)和探诊出血(bleeding on probing,BOP)阳性率及X线评估骨吸收。4周后处死大鼠,RT-qPCR检测牙龈组织中IL-1β、IL-10 mRNA的表达并计算IL-1β/IL-10比值,分析其与临床和影像学指标的相关性。结果:体外炎症刺激显著上调IL-1β和IL-10 mRNA表达(P<0.05)。动物实验模型组PPD、BOP阳性率及骨吸收量均显著高于对照组(P<0.05),且 IL-1β mRNA 表达增加,IL-10 mRNA表达减少,IL-1β/IL-10比值升高(P<0.05)。相关性分析结果显示IL-1β及IL-1β/IL-10比值与PPD和骨吸收量呈正相关,IL-10与二者呈负相关(P<0.05)。结论:本研究成功构建大鼠PID模型,揭示了IL-1β和IL-10与其临床和影像学指标的相关性,为阐明PID发病机制及开发炎症因子靶向治疗策略提供了实验依据。

关键词: 种植体周围病, 大鼠模型, 炎症因子, 白细胞介素-1β, 白细胞介素-10

Abstract: Objective: To establish a rat model of peri-implant disease (PID) based on changes in inflammatory factors in vitro, and to investigate the correlation between interleukin-1β (IL-1β) and interleukin-10 (IL-10) and clinical/radiographic parameters. Methods: In vitro, human monocytic leukemia (THP-1) cells were stimulated with lipopolysaccharide (LPS) and interferon-gamma (IFN-γ) to simulate an inflammatory microenvironment. Real-time fluorescence quantitative PCR (RT-qPCR) was used to detect mRNA expression of IL-1β and IL-10. In vivo, custom implants were placed mesial to the maxillary first molars in 20 Sprague-Dawley (SD) rats. Four weeks later, 12 rats with successful osseointegration were selected and divided into a control group and a model group (n=6 each). PID was induced in the model group via ligation and topical application of Porphyromonas gingivalis (P.g) suspension, while the control group received no intervention. Probing pocket depth (PPD), positive rate of bleeding on probing (BOP), and radiographic bone resorption were recorded weekly. After 4 weeks, rats were euthanized. RT-qPCR was performed to detect IL-1β and IL-10 mRNA expression in gingival tissues, the IL-1β/IL-10 ratio was calculated, and their correlations with clinical and radiographic parameters were analyzed. Results: In vitro inflammatory stimulation significantly upregulated IL-1β and IL-10 mRNA expression (P<0.05). In the animal experiment, the model group exhibited significantly higher PPD, BOP positive rate, and bone resorption compared to the control group (P<0.05). Furthermore, IL-1β mRNA expression was increased, IL-10 mRNA expression was decreased, and the IL-1β/IL-10 ratio was increased in the model group (all P<0.05). Correlation analysis revealed that IL-1β and the IL-1β/IL-10 ratio were positively correlated with PPD and bone resorption, while IL-10 was negatively correlated with both (all P<0.05). Conclusion: This study successfully established a rat PID model. It revealed significant correlations between IL-1β, IL-10, and clinical/radiographic parameters, providing an experimental foundation for elucidating the pathogenesis of PID and developing targeted therapeutic strategies focusing on inflammatory cytokines.

Key words: peri-implant disease, rat model, inflammatory cytokines, interleukin-1β, interleukin-10

梅龙, 蔡程晨, 董淑雅, 郑雨翔, 何俊刚, 程鲁晋. 大鼠种植体周围病IL-1β及IL-10与临床和影像学指标相关性研究[J]. 口腔医学研究, 2026, 42(3): 195-200.

MEI Long, CAI Chengchen, DONG Shuya, ZHENG Yuxiang, HE Jungang, CHENG Lujin. Correlation Study between IL-1β and IL-10 with Clinical and Radiographic Parameters in Peri-implant Disease of Rats[J]. Journal of Oral Science Research, 2026, 42(3): 195-200.

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