口腔医学研究

口腔医学研究 ›› 2026, Vol. 42 ›› Issue (6): 504-511.DOI: 10.13701/j.cnki.kqyxyj.2026.06.008

• 口腔颌面外科学研究 • 上一篇    下一篇

3D打印HA-SA-CMCS三维支架联合Masquelet技术重建骨缺损的研究

王建哲, 朱骁菁, 陈玲玲, 施振宇, 王忆冰, 冯成, 李琼, 杜麒豪, 吴烨*   

  1. 福建省口腔疾病研究重点实验室;福建省高校口腔医学重点实验室;福建省口腔组织缺损性疾病临床医学研究中心;福建医科大学口腔医学院·附属口腔医院口腔颌面外科 福建 福州 350002
  • 收稿日期:2025-09-19 出版日期:2026-06-28 发布日期:2026-06-23
  • 通讯作者: *吴烨,E-mail:wuye@fjmu.edu.cn
  • 作者简介:王建哲(1998~),男,山西运城人,硕士,研究方向:口腔颌面外科学。
  • 基金资助:
    福建省财政厅科技专项(编号:2023CZZX04);福建省自然科学基金项目(编号:2025J01814);福建省口腔组织缺损性疾病临床医学研究中心课题项目(编号:2024Clin004)

Study on Reconstruction of Bone Defects by 3D-Printed HA-SA-CMCS Three-Dimensional Scaffold Combined with Masquelet Technique

WANG Jianzhe, ZHU Xiaojing, CHEN Lingling, SHI Zhenyu, WANG Yibing, FENG Cheng, LI Qiong, DU Qihao, WU Ye*   

  1. Fujian Key Laboratory of Oral Diseases; Stomatological Key Laboratory of Fujian College and University; Clinical Research Center for Oral Tissue Deficiency Diseases of Fujian Province; Department of Oral and Maxillofacial Surgery, Affiliated Stomatological Hospital, Fujian Medical University, Fuzhou 350002, China
  • Received:2025-09-19 Online:2026-06-28 Published:2026-06-23

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摘要： 目的:针对自体骨移植用于临床骨缺损治疗存在开辟第二术区、疼痛、感染、取骨量有限等问题,结合骨组织工程理念与Masquelet技术制备具有良好血供条件的羟基磷灰石/海藻酸钠/羧甲基壳聚糖(hydroxyapatite/sodium alginate/carboxymethyl chitosan, HA-SA-CMCS,简称HSC)三维支架,并评估其骨修复能力。方法:利用3D打印技术制备HSC三维支架,通过扫描电镜、傅里叶变换红外光谱仪、X射线衍射、万能试验机等方法检测支架机械性能、体外降解性及体外矿化能力等相关表征;通过兔骨髓间充质干细胞(bone marrow mesenchymal stem cells, BMSCs)行相关细胞实验、碱性磷酸酶(alkaline phosphatase, ALP)及茜素红(alizarin red S, ARS)染色评估支架体外生物相容性及体外成骨矿化性能;最后通过构建新西兰兔颅骨缺损模型评价支架体内成骨性能。结果:HSC支架具有良好的机械性能、亲水性及生物相容性;ALP及ARS染色结果显示HSC支架具有良好的促进兔BMSCs成骨分化能力。动物实验结果同样提示相比空白对照组,HSC支架组具备更优异的骨修复性能。结论:3D打印HSC三维支架联合Masquelet技术能有效促进骨组织再生,加速兔颅骨缺损修复,为骨缺损治疗开辟了一条极具潜力的新途径。

关键词: 3D打印, 骨缺损, Masquelet技术, 骨组织工程

Abstract: Objective: To develope a hydroxyapatite/sodium alginate/carboxymethyl chitosan (HA-SA-CMCS, referred to as HSC) three-dimensional scaffold with favorable blood supply potential by integrating tissue engineering principles with the Masquelet technique, and evaluate its bone repair efficacy. Methods: HSC three-dimensional scaffolds were fabricated using 3D printing. The mechanical properties, in vitro degradability, and mineralization capacity of the scaffolds were characterized using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), a universal testing machine, and other techniques. The in vitro biocompatibility and osteogenic potential were assessed through cell culture with rabbit bone marrow mesenchymal stem cells (BMSCs), including alkaline phosphatase (ALP) and alizarin red S (ARS) staining. Finally, the in vivo osteogenic performance was evaluated using a skull defect model in New Zealand white rabbits. Results: The HSC scaffold exhibited excellent mechanical properties, hydrophilicity, and biocompatibility. ALP and ARS staining demonstrated that the HSC scaffold significantly promoted the osteogenic differentiation of rabbit BMSCs. Animal experiments further indicated that the HSC scaffold group had superior bone repair performance compared to the blank control group. Conclusion: The 3D-printed HSC three-dimensional scaffold combined with the Masquelet technique can effectively promote bone tissue regeneration, accelerate the repair of rabbit skull defects, and open up a highly promising new approach for the treatment strategy of bone defects.

Key words: 3D printing, bone defect, masquelet technique, bone tissue engineering