聚乳酸/羟基磷灰石(PLLA/HA)复合物预成乳前牙根管桩的生物相容性研究

口腔医学研究 ›› 2015, Vol. 31 ›› Issue (11): 1073-1077.

聚乳酸/羟基磷灰石(PLLA/HA)复合物预成乳前牙根管桩的生物相容性研究

盖立婷,徐高祥,张鲁鲁,高翔,姜秋^*

吉林大学口腔医学院儿童口腔科吉林长春 130021

收稿日期:2015-04-08 出版日期:2015-11-28 发布日期:2016-03-21
通讯作者: 姜秋,电话:0431-88796038
作者简介:盖立婷(1990~),女,内蒙古人,硕士在读,主要从事儿童口腔医学研究工作。
基金资助:
吉林省工信厅项目(编号:2013220101000394)

Biocompatibility of Polylactic Acid/hydroxyapatite (PLLA/HA) Composites as Root Canal Post in Primary Anterior Teeth.

GAI Li-ting, XU Gao-xiang, ZHANG Lu-lu, Gao Xiang, Jiang Qiu.

Department of Pedodontics, School and Hospital of Stomatology, Jilin University, Changchun 130021, China

Received:2015-04-08 Online:2015-11-28 Published:2016-03-21

摘要/Abstract

摘要： 目的:评估聚乳酸/羟基磷灰石(PLLA/HA)复合物的生物相容性。方法:本实验主要参照我国医疗器械生物学评价标准,采用细胞黏附实验,溶血试验,急性毒性实验,热源实验和肌肉植入实验等系列体内外实验评价PLLA/HA的生物相容性。结果:L929细胞在材料表面生长良好;材料浸提液对实验兔不引起溶血反应、无致热源性;对小鼠无急性毒性;PLLA和PLLA/HA板块植入实验兔肌肉后,早期组织周围有轻微炎症反应,形成较厚纤维膜,随植入时间延长,炎症反应减轻,纤维包膜基本消失;SEM显示:初始纯PLLA结构致密,PLLA/HA中HA粒子大小均一,分布均匀,随降解时间延长,材料出现孔隙和裂纹。结论:PLLA/HA复合材料具有良好的生物相容性,符合生物安全性要求。

Abstract: Objective: To evaluate the biocompatibility of polylactic acid/hydroxyapatite(PLLA/HA) composites. Methods: According to the standard for biological evaluation of medical devices, a series of tests were performed in vivo and in vitro to evaluate the biocompatibility of PLLA/HA composites, such as cell adhesion test, hemolysis test, acute toxicity test, pyrogen test, and muscle implantation test. Results: L929 cells grew well on the PLLA/HA composites’ surface.The leaching liquor of PLLA/HAcomposites could not cause the hemolytic reaction and pyrogen reaction in rabbits and no acute toxicity in mice.The PLLA/HA composites which were implanted in rabbits’ muscle only caused a mild inflammatory reaction and a thick fiber capsule during the early stage,which subsided gradually.SEM showed that PLLA had a compact structure initially,the grains on the PLLA/HA composites were of uniform size and evenly distributed,some porous structures and cracks were observed gradually. Conclusion: The PLLA/HA composites had a good biocompatibility and met the requirement of biological safety.

中图分类号:

R788

盖立婷,徐高祥,张鲁鲁,高翔,姜秋. 聚乳酸/羟基磷灰石(PLLA/HA)复合物预成乳前牙根管桩的生物相容性研究[J]. 口腔医学研究, 2015, 31(11): 1073-1077.

GAI Li-ting, XU Gao-xiang, ZHANG Lu-lu, Gao Xiang, Jiang Qiu.. Biocompatibility of Polylactic Acid/hydroxyapatite (PLLA/HA) Composites as Root Canal Post in Primary Anterior Teeth.[J]. Journal of Oral Science Research, 2015, 31(11): 1073-1077.

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