卫星胶质细胞活化在束缚应激致大鼠咬肌痛觉敏感中的作用

口腔医学研究 ›› 2015, Vol. 31 ›› Issue (12): 1174-1178.

卫星胶质细胞活化在束缚应激致大鼠咬肌痛觉敏感中的作用

刘杨¹,苗莉²,赵雅娟¹,王舰³,张旻¹,赵蕊妮¹,陈永进^1*,赵文峰^2*

1. 军事口腔医学国家重点实验室,第四军医大学口腔医院急诊与综合临床科陕西西安 710032;
2. 北京军区总医院口腔科北京 100700;
3. 第四军医大学基础部人体解剖与组织胚胎学教研室陕西西安 710032

收稿日期:2015-05-14 出版日期:2015-12-28 发布日期:2016-03-21
通讯作者: 陈永进,E-mail:cyj1229@fmmu.edu.cn
赵文峰,E-mail:liuli046@gmail.com
作者简介:刘杨(1988~),女,陕西宝鸡人,硕士,主要从事颞下颌关系紊乱机制研究。
基金资助:
国家自然科学基金项目(编号:81271167)
军事口腔医学国家重点实验室开放课题(编号:2014KA02)

Effects of Satellite Glia Cells Activation in Masseter Allodynia Induced by Restraint Stress in Rats.

LIU Yang¹, MIAO Li², ZHAO Ya-juan¹, WANG Jian³, ZHANG Min¹, ZHAO Rui-ni¹, CHEN Yong-jin¹, ZHAO Wen-feng².

1. State Key Laboratory of Military Stomatology, Department of General Dentistry and Emergency ＆ School of Stomatology, The Fourth Military Medical University, Xi'an 710032, China;
2. Department of Stomatology, General Hospital of Beijing Military Command, Beijing 100700, China;
3. Department of Human Anatomy, Histology and Embryology, School of Basic Medicine, The Fourth Military Medical University, Xi'an 710032, China

Received:2015-05-14 Online:2015-12-28 Published:2016-03-21

摘要/Abstract

摘要： 目的:观察慢性心理应激致咬肌痛觉敏感中三叉神经节卫星胶质细胞活化和P物质释放的关系。方法:40只健康雄性SD大鼠随机分为空白对照组、束缚应激3 d组、束缚应激7 d组、束缚应激14 d组。用体质量检测、旷场实验观察束缚应激后大鼠是否产生焦虑、抑郁样情绪;用Von Frey电子测痛仪检测大鼠咬肌痛阈。用免疫组织荧光染色法检测三叉神经节卫星胶质细胞活化的标志物神经胶质酸性蛋白(Glial fibrillary acidic protein, GFAP)及神经肽P物质(Substance P, SP)免疫阳性产物的变化。结果:受束缚应激后,大鼠体质量增长明显低于对照组,在旷场中总移动距离和总移动速度均明显降低,提示大鼠处于焦虑、抑郁样情绪状态。束缚应激后大鼠咬肌机械性痛阈明显降低;提示大鼠咬肌痛敏增强。束缚应激7 d、14 d组大鼠三叉神经节内GFAP及SP的表达明显高于空白对照组。结论:慢性心理应激可导致三叉神经节中的卫星胶质细胞活化,神经元SP表达升高。因此,三叉神经节中卫星胶质细胞和神经元的活化可能参与了慢性心理应激引起咬肌痛觉敏感升高过程。

关键词: 心理应激, 痛觉敏感, 三叉神经节, 卫星胶质细胞, GFAP, SP

Abstract: Objective: To investigate the role of satellite glia cells (SGCs) activation and substance P (SP) expression in the trigeminal ganglion (TG) in the increased masseter mechanical sensitivity induced by psychological stress in rats. Methods: Forty male Sprague-Dawley rats were randomly divided into control group, 3-day, 7-day, and 14-day stress group (n=10). The rats were subjected to restraint stress 6 hours per day. The body weight gain and open field test were employed to evaluate the animals' stress condition. The electronic Von-Frey anesthesiometer were used to evaluate the masseter mechanical sensitivity. The expression of glial fibrillary acidic protein (GFAP) and SP was detected by immunofluorescent staining. Results: The rats in the stress group showed significant slower body weight increase, anxious and depressive behavior compared to control group. The masseter mechanical sensitivity of rats in the stress group was significantly elevated. The expression levels of GFAP and SP in TG were significantly up-regulated in the stress groups compared with control group. Conclusion: Chronic restraint stress could result in satellite glia cells activation and elevated SP expression in TG, indicating that the satellite glia cells activation may be involved in the increased masseter mechanical sensitivity induced by psychological stress in rats.

中图分类号:

R745.1⁺1

刘杨,苗莉,赵雅娟,王舰,张旻,赵蕊妮,陈永进,赵文峰. 卫星胶质细胞活化在束缚应激致大鼠咬肌痛觉敏感中的作用[J]. 口腔医学研究, 2015, 31(12): 1174-1178.

LIU Yang, MIAO Li, ZHAO Ya-juan, WANG Jian, ZHANG Min, ZHAO Rui-ni, CHEN Yong-jin, ZHAO Wen-feng.. Effects of Satellite Glia Cells Activation in Masseter Allodynia Induced by Restraint Stress in Rats.[J]. Journal of Oral Science Research, 2015, 31(12): 1174-1178.

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