口腔医学研究

口腔医学研究 ›› 2016, Vol. 32 ›› Issue (6): 584-587.DOI: 10.13701/j.cnki.kqyxyj.2016.06.008

• 临床研究论著 • 上一篇    下一篇

炎症作用下Caspase-3/8对人牙龈间充质干细胞凋亡能力影响的研究

张洋1* ,刘杰2# ,石海刚3 ,李晓光1 ,张维3 ,刘娜1 ,徐璐璐1   

  1. 1. 解放军总医院口腔医学中心 北京 100853;
    2. 解放军第309医院小西天门诊部 北京 100091;
    3. 中国科学院理化技术研究所 北京 100101
  • 收稿日期:2016-01-22 出版日期:2016-06-26 发布日期:2016-06-22
  • 通讯作者: 徐璐璐,E-mail:xululu1977@163.com
    #为共同第一作者
  • 作者简介:张洋(1984~ ),男,北京人,硕士,医师,主要从事口腔正畸临床治疗工作。
  • 基金资助:
    国家自然科学基金(编号:31200741,51473175)
    北京科技新星计划(编号:Z14111000180000)
    解放军总医院临床科研扶持基金(2013FC-TSYS-2007)

Effect of Caspase-3/8 on Apoptosis of Human Gingival Mesenchymal Stem Cells under Inflammation.

ZHANG Yang1*, LIU Jie2#, SHI Hai-gang3, LI Xiao-guang1, ZHANG Wei3, LIU Na1, XU Lu-lu1.   

  1. 1. Stomatological Center,Chinese PLA General Hospital,Beijing 100853, China; 2. The 309th Hospital of Chinese People's Libertation Army, Beijing 100091, China;
    3. Technical Institute of Physics and Chemistry CAS, Beijing 100101, China
  • Received:2016-01-22 Online:2016-06-26 Published:2016-06-22

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摘要： 目的:探讨炎症作用下Caspase-3/8对人牙龈间充质干细胞凋亡能力影响的研究,为探索牙龈炎的预防和治疗策略提供理论和实验依据。方法:取20～25岁拔除阻生齿牙切除的无炎症龈瓣和正畸过程中增生的牙龈组织,分离培养正常牙龈间充质干细胞(NGMSCs)和炎性牙龈间充质干细胞(IGMSCs)。AnnexinV-FITC/PI双染检测相同培养条件下NGMSCs与IGMSCs凋亡率;应用PCR,免疫荧光法检测各组间Caspase-3,Caspase-8因子表达差异。结果:AnnexinV-FITC/PI双染检测结果显示相同培养条件下IGMSCs凋亡率(10.44%)>NGMSCs凋亡率(2.76%)。PCR结果显示IGMSCs组Caspase-3,Caspase-8表达显著高于NGMSCs组(P<0.01)。IGMSCs组TNF-α,IL-1β基因表达显著高于NGMSCs组(P<0.01)。结论:Caspase-3/8在牙龈间充质干细胞增殖、凋亡途径中起着重要调控作用。

关键词: 牙龈间充质干细胞, 炎性, 凋亡, Caspase-3, Caspase-8

Abstract: Objective: To explore the the effect of Caspase-3/8 on apoptosis of human gingival mesenchymal stem cells under inflammation. Methods: Gingival tissues with or without inflammation were obtained from the third impacted molars and gingival hyperplasia because of orthodontic treatment, respectively. Patients were all about 20-25 years old. Normal gingival mesenchymal stem cells (NGMSCs) and inflammatory gingival mesenchymal stem cells (IGMSCs) were isolated and cultured. Apoptosis rates of IGMSCs and NGMSCs were determined by V-FITC/PI Annexin double staining. Caspase-3 and Caspase-8 expression differences were detected by Real-Time PCR. Results: Apoptosis rate of IGMSCs (10.44%) was significantly more than that of NGMSCs (2.76%). Expressions of Caspase-3 and Caspase-8 in IGMSCs group were significantly higher than those of NGMSCs group (P<0.01) as well as TNF- α and IL-1β gene expressions. Conclusion: Caspase-3/8 plays an important role in regulating the proliferation and apoptosis of mesenchymal stem cells.

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