血卟啉单甲醚荧光光谱法辅助诊断早期口腔癌

口腔医学研究 ›› 2015, Vol. 31 ›› Issue (7): 699-701.

血卟啉单甲醚荧光光谱法辅助诊断早期口腔癌

杨艳青¹,袁杰^1*,张磊¹,叶珑伟¹,黄鑫¹,张晓丹¹,李楠²

1. 哈尔滨医科大学附属第一医院口腔预防保健科黑龙江哈尔滨 150001;
2. 哈尔滨医科大学附属第一医院口腔颌面外科

收稿日期:2014-12-04 出版日期:2015-07-28 发布日期:2015-07-28
通讯作者: 袁杰,E-mail:dryuanjie3982@163.com
作者简介:杨艳青(1988~ ),女,河北人,硕士在读,主要从事口腔预防保健临床及基础的研究工作。
基金资助:
哈医大一院科研基金(项目编号:2014B19)

Hematoporphyrinmonomethyl Ether Fluorescence Spectrometry Auxiliary Diagnosis of Early Oral Cancer

YANG Yan-qing, YUAN Jie, ZHANG Lei, et al

Department of Preventive Dentistry, The First Affiliated Hospital, Harbin Medical University, Harbin150001

Received:2014-12-04 Online:2015-07-28 Published:2015-07-28

摘要/Abstract

摘要： 目的:应用动物模型探讨血卟啉单甲醚(hematoporphyrinmonomethyl ether,HMME)介导的荧光光谱法辅助诊断早期口腔癌的可行性。方法:21只金黄地鼠随机分成3组(对照组、5周组、9周组),每组7只,各组动物由DMBA诱导双侧颊囊相应时间后,按10 ml/kg经腹腔注射20 mg/L HMME溶液,用光谱诊断装置分别记录自体及HMME荧光,应用Origin7.0软件计算荧光比值K值,检测位点经病理切片HE染色定性.计算各组K值均值,SPSS10.0统计分析。结果:动物实验结果经方差分析,3组K值均值相比有统计学意义(P<0.01)。结论:HMME介导的荧光光谱法能够区分正常、异常增生和癌变组织,对口腔早期癌的诊断具有重要参考价值。

关键词: 血卟啉单甲醚, 光谱, 口腔癌, 诊断, 金黄色地鼠

Abstract: Objective: To investigate the feasibility of fluorescence spectra diagnosis of early oral cancer mediated with hematoporphyrinmonomethyl ether(HMME) in animal models. Methods: 21 golden hamsters were randomly divided into 3 groups (control group, 5 weeks group and 9 weeks group). Each group had 7 animals. After induced by DMBA in corresponding weeks all the cheek pouches of animal models in each group were injected through intraperitoneal by 20ug/ml HMME solution as 10ml/kg. HMME fluorescence and auto-fluorescence spectrums were detected by fluorescence spectroscopy diagnosis system and were analyzed by Origin7.0 to calculate fluorescence ratio K. All the detected points were determined by pathological diagnose. Mean K values were calculated and statistically analyzed by SPSS10.0. Results: The result of animal experiment was analyzed by ANOVA. It showed that the comparisons of K values among 3 experiment groups had statistical significance(P<0.01). Conclusion: Fluoresscent-spectro fluorometry method mediated by HMME could discriminate normal tissue, abnormal hyperplasia and cancer tissue. It had important reference value in diagnosis of early oral cancer.

Key words: Hematoporphyrinmonomethyl ether, Spectroscopy, Oral cancer, Diagnose, Golden hamsters

中图分类号:

R739.8

杨艳青,袁杰,张磊,叶珑伟,黄鑫,张晓丹,李楠. 血卟啉单甲醚荧光光谱法辅助诊断早期口腔癌[J]. 口腔医学研究, 2015, 31(7): 699-701.

YANG Yan-qing, YUAN Jie, ZHANG Lei, et al. Hematoporphyrinmonomethyl Ether Fluorescence Spectrometry Auxiliary Diagnosis of Early Oral Cancer[J]. Journal of Oral Science Research, 2015, 31(7): 699-701.

参考文献

[1] 孟宪瑞,刘进忠.口腔癌的早期诊断[J].国际口腔医学杂志,2008,35(3)∶329-331
[2] Johnson NW, Warnakulasuriya S, Gupta PC, et al. Global oral health inequalities in incidence and outcomes for oral cancer: causes and solutions [J]. Adv Dent Res, 2011, 23(2)∶237-246
[3] Nagini S,Letchoumy PV,A T Cr R.Of humans and hamsters:a comparative evaluation of carcinogen activation,DNA damage,cell proliferation,apoptosis,invasion,and angiogenesis in oral cancer patients and hamster buccal pouch carcinomas [J].Oral Oncol,2009,45(6)∶e31-37
[4] Gillenwater A, Papadimitrakopoulou V, Richards-Kortum R. Oral premalignancy: new methods of detection and treatment [J]. Curr Oncol Rep,2006,8(2)∶146-154
[5] 邱蔚六.口腔颌面肿瘤学[M].山东科学技术出版社,2003,34-35
[6] Rajaram N, Reichenberg JS, Migden MR, et al. Pilot clinical study for quantitative spectral diagnosis of non-melanoma skin cancer [J]. Lasers Surg Med,2010,42(10)∶716-827
[7] Kraft M, Betz CS, Leunig A, Arens C. Value of fluorescence endoscopy for the early diagnosis of laryngeal cancer and its precursor lesions [J]. Head Neck, 2011, 33(7)∶941-948
[8] Konopka K, Goslinski T. Photodynamic therapy in dentistry [J]. J Dent Res, 2007, 86(8)∶694-707
[9] Kobayashi W, Liu Q, Nakagawa H, et al. Photodynamic therapy with mono-L-aspartyl chlorin e6 can cause necrosis of squamous cell carcinoma of tongue: experimental study on an animal model of nude mouse [J]. Oral Oncol, 2006, 42(1)∶46-50
[10] Gu Y, Huang N Y, Liang J, et al. Clinical study of 1949 cases of port wine stains treated with vascular photodynamic therapy( Gu’s PDT) [J]. Ann Dermatol Venereol,2007,134( 31)∶241-244
[11] Wang J, Liu L, Pan S, et al. Therapeutic effect of photodynamic therapy using hematoporphyrin monomethyl ether (HMME) on human cholangiocarcinoma cell line QBC939 [J]. Neoplasma, 2009, 57(1)∶79-85
[12] Cai H, Gu Y, Sun Q, Zeng J, Dong N, Zhao G. Effect of hematoporphyrinmonomethyl ether-mediated photodynamic therapy on hypertrophic scar fibroblasts [J]. Photodermat, Photoimmunol Photomed,2011,27(2)∶90-96
[13] HeY, GeH, Li S. Haematoporphyrin based photodynamic therapy combined with hyperthermia provided effective therapeutic vaccine effect against colon cancer growth in mice [J]. Int J Med Sci,2012,9(8)∶627
[14] Sun Y, Xing D, Shen L, et al. Bactericidal effects of hematoporphyrin monomethyl ether-mediated photosensitization against pathogenic communities from supragingival plaque [J]. Appl Microbiol Biotechnol,2013,97(11)∶5079-5087
[15] MacDonald DG. Comparison of epithelial dysplasia in hamster cheek pouch carcinogenesis and human oral mucosa [J]. J Oral Pathol, 1981, 10(3)∶186-191