富血小板血浆对骨髓间充质干细胞成骨向分化的作用

口腔医学研究 ›› 2015, Vol. 31 ›› Issue (7): 735-738.

富血小板血浆对骨髓间充质干细胞成骨向分化的作用

李斯翰,段建民^*,文军,李洪涛

广州军区广州总医院口腔科广东广州 510000

收稿日期:2014-11-27 出版日期:2015-07-28 发布日期:2015-07-28
通讯作者: 段建民,E-mail:duanjianmin3@126.com
作者简介:李斯翰(1987~ ),女,广东汕头人,医师,硕士在读,主要从事牙体牙髓病的临床治疗工作。

Effect of Modified Platelet-rich Plasma on the Mineralization of Human Bone Marrow Stromal Cells

LI Shi-han, DUAN Jian-min, WEN Jun, et al

Department of Stomatology, Guangzhou General Hospital of Guangzhou Military Region, Guangzhou 510000

Received:2014-11-27 Online:2015-07-28 Published:2015-07-28

摘要/Abstract

摘要： 目的:观察不同浓度改良富血小板血浆(PRP)对骨髓间充质干细胞(BMSCs)成骨向分化的作用效果。方法:使用健康志愿者骨髓培养的3~4代BMSCs。将由该志愿者静脉采集的静脉血制备成改良PRP作用于BMSCs,使用碱性磷酸酶(ALP)试剂盒测定矿化诱导7 d后BMSCs的ALP活性,通过茜素红染色观察矿化诱导21 d后BMSCs的矿化结节形成情况,并通过实时荧光定量PCR检测矿化诱导7 d后BMSCs中成骨相关基因Runx2表达的变化。结果:5%改良PRP明显提高了BMSCs的ALP活性,促进了其矿化结节的形成,并上调了BMSCs中Runx2基因的表达。结论:液氮冻融激活的改良PRP促进BMSCs成骨向分化具有浓度特异性。

关键词: 富血小板血浆, 骨髓间充质干细胞, 成骨向分化

Abstract: Objevtive: To investigate the effect of platelet-rich plasma (PRP) at different concentrations on the mineralization of human bone marrow stromal cells. Methods: BMSCs from healthy donor were cultured and passaged for 3-4 passages. PRP were manufactured from venous blood of the same donor. The activity of alkaline phosphatase in BMSCs was detemined using alkaline phosphatase reagent kit on the 7th day after mineralization induction. The formation of mineralized nodules in BMSCs were observed by alizarin red staining on the 21th days after minralization induction. After 7 days of minralization induction the expression of gene Runx2 in BMSCs was measured by real-time PCR. Results: PRP at the concentration of 5% evidently promoted the alkain phosphatase activity and the formation of mineralized nodules in BMSCs. The expression of gene Runx-2 was also evidently enhanced. Conclusion: PRP at the concentration of 5% could promote the mineralization of BMSCs.

Key words: PRP, BMSCs, Mineralization

中图分类号:

R781.4

李斯翰,段建民,文军,李洪涛. 富血小板血浆对骨髓间充质干细胞成骨向分化的作用[J]. 口腔医学研究, 2015, 31(7): 735-738.

LI Shi-han, DUAN Jian-min, WEN Jun, et al. Effect of Modified Platelet-rich Plasma on the Mineralization of Human Bone Marrow Stromal Cells[J]. Journal of Oral Science Research, 2015, 31(7): 735-738.

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