口腔医学研究

口腔医学研究 ›› 2015, Vol. 31 ›› Issue (9): 927-930.

• 临床研究论著 • 上一篇    下一篇

microRNA-21在口腔鳞状细胞癌中的表达与预后关系

常粲然*,杜军,李二红,杨勇   

  1. 焦煤中央医院口腔科 河南 焦作 454000
  • 收稿日期:2015-02-02 出版日期:2015-09-28 发布日期:2016-05-04
  • 通讯作者: 常粲然,电话:13203914844
  • 作者简介:常粲然(1979~ ),男,学士,主治医师,主要从事口腔外科的临床研究工作。

Expression of MicroRNA-21 in Oral Squamous Cell Carcinoma and Its Correlation with Prognosis

CHANG Can-ran, DU Jun, LI Er-hong, YANG Yong   

  1. Department of Stomatology, Jiaomei Central Hospital, Jiaozuo 454150, China
  • Received:2015-02-02 Online:2015-09-28 Published:2016-05-04

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摘要： 目的:探讨microRNA-21在口腔鳞状细胞癌中的表达情况,以及与预后关系。方法:选取98例口腔鳞状细胞癌患者组织及正常黏膜组织标本,同时,选取50例非肿瘤患者黏膜组织,分别提取标本中总RNA,利用RT-PCR检测microRNA-21在口腔鳞状细胞癌及正常黏膜组织中相对表达量,探讨其与临床病理特征的关系,利用Kaplan-Meier法分析其与预后的关系。结果:microRNA-21在口腔鳞状细胞癌组织中相对表达量(2.37±1.01),显著高于正常黏膜组织中的(0.97±0.08)和非肿瘤患者黏膜组织中的(0.94±0.07),差异具有统计学意义(P<0.05),;microRNA-21表达与患者性别、年龄、肿瘤位置、肿瘤大小和TNM分期无关,而与分化程度和是否发生淋巴结转移有关(P<0.05);将microRNA-21相对表达量的中位值2.41为界,将所有患者分为高表达组和低表达组,高表达组患者中位生存期38个月,低表达组患者中位生存期44个月,差异具有统计学意义(P<0.05)。结论:microRNA-21在口腔鳞状细胞癌中呈高表达,且与分化程度和淋巴结转移有关,并可影响患者预后。

关键词: 口腔鳞状细胞癌, 微RNA-21, 表达, 预后

Abstract: Objective: To investigate the expression of microRNA-21 in oral squamous cell carcinoma and its correlation with prognosis. Methods: 98 cases of oral squamous cell carcinoma tissue and normal tissue samples were selected. Meanwhile, mucosa of 50 cases of non-cancer patients was selected. Total RNA was isolated from specimens, respectively. The relative expression levels of microRNA-21 in oral squamous cell carcinoma and normal mucosa were detected by RT-PCR. Its correlations with clinicopathological features and prognosis were analyzed by Kaplan-Meier method. Results: The relative expression level of microRNA-21 in oral squamous cell carcinoma (2.37±1.01) was significantly higher (P<0.05) than normal mucosa (0.97±0.08) and non-cancer patients mucosa (0.94±0.07). The expression of microRNA-21 was not correlated with sex, age, tumor location, tumor size and TNM stage, but significantly correlated with the degree of differentiation and lymph node metastasis (P<0.05). All patients were divided into high and low expression groups according to the median value of microRNA-21 relative expression (2.41). The median survival of patients was 38 months in high expression group, while 44 months in low expression group, and the difference was statistically significant (P<0.05). Conclusion: The expression level of microRNA-21 was elevated in oral squamous cell carcinoma, which was correlated with the degree of differentiation and lymph node metastasis, and affected the prognosis of patients.

Key words: Oral squamous cell carcinoma, MicroRNA-21, Prognosis

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