口腔医学研究

口腔医学研究 ›› 2016, Vol. 32 ›› Issue (2): 172-176.DOI: 10.13701/j.cnki.kqyxyj.2016.02.017

• ·临床研究论著· • 上一篇    下一篇

慢性根尖周炎中Wnt5a与骨吸收的关系

徐莉雅 马楠 仇丽鸿* 于雅琼 于静涛 薛明   

  1. 1. 中国医科大学口腔医学院牙体牙髓病科 辽宁 沈阳 110002;
    2. 辽宁省口腔医学研究所牙体牙髓病研究室 辽宁 沈阳 110002;
    3. 辽宁省口腔疾病转化医学研究中心 辽宁 沈阳 110002
  • 收稿日期:2015-12-15 出版日期:2016-02-28 发布日期:2016-03-10
  • 通讯作者: 仇丽鸿,电话:024-22895932
  • 作者简介:徐莉雅(1983~),女,辽宁本溪人,硕士,医师,主要从事牙体牙髓病学的研究工作。
  • 基金资助:
    辽宁省自然科学基金资助项目(编号:2014021055)

Relationship between Wnt5a and Bone Resorption in Chronic Apical Periodontitis.

XU Li-ya, MA Nan, QIU Li-hong, YU Ya-qiong, Yu Jing-tao, Xue Ming   

  1. 1. Department of Endodontics, School of Stomatology, China Medical University. Shenyang 110002, China;
    2. Lab of Endodontics, Liaoning Provincial Institute of Stomatology. Shenyang 110002, China;
    3. Research Center of Translational Medicine, Liaoning Provincial Oral Disease. Shenyang 110002, China
  • Received:2015-12-15 Online:2016-02-28 Published:2016-03-10

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摘要： 目的:检测Wnt5a在慢性根尖周炎病损中的表达以及Wnt5a与病变范围的关系。方法:18例慢性根尖周炎患者,根据病变大小分为A组(d≥6 mm)、B组(6 mm＞d≥3 mm),21例因正畸需要而拔除的健康牙作对照组,采用RT-PCR(Real-Time PCR)法,从mRNA水平检测Wnt5a的表达,分析Wnt5a mRNA表达与临床病变范围的关系。A组、B组及对照组各随机抽取5例进行免疫组化。采用SPSS13.0软件包对数据进行统计学分析。结果:RT-PCR结果中病例组Wnt5a mRNA表达水平显著高于对照组(P＜0.05);A组中Wnt5a mRNA表达水平显著高于对照组(P＜0.01);A组中Wnt5a mRNA表达水平与B组相比(P＜0.01);B组中Wnt5a mRNA表达水平与正常对照组相比差异无统计学意义(P＞0.05)。免疫组化结果中病例组Wnt5a表达水平显著高于正常对照组(P＜0.01),A组Wnt5a的表达水平显著高于正常对照组(P＜0.01);B组Wnt5a的表达水平高于正常对照组(P＜0.05);A组中Wnt5a表达水平高于B组(P＜0.05)。结论:Wnt5a与慢性根尖周炎的骨组织吸收相关,可能促进了骨吸收的发展。

关键词: 慢性根尖周炎, Wnt5a, RT-PCR法, 免疫组化法, 骨吸收

Abstract: Objective: To detect the expression of Wnt5a mRNA in lesions of chronic apical periodontitis. Methods: 18 patients of chronic apical periodontitis were divided into group A (d≥6 mm) and group B (6 mm＞d≥3 mm) according to the scope of the root lesions. 21 healthy teeth extracted for orthodontic reasons were selected as the control group. Expression of Wnt5a mRNA was analyzed with RT-PCR (Real-Time PCR) to identify its relationship with the scope of the lesion. 10 patients of chronic apical periodontitis and 5 healthy controls were chosen for immunohistochemistry. All statistical analysis was performed using the SPSS 13.0 statistical package. Results: Wnt5a mRNA expression in patients increased significantly than that in normal controls (P<0.05). Compared with control group, group A had a significant increase in Wnt5a mRNA expression (P<0.01). The difference between group A and group B was also significant (P<0.01). While there was no significant difference between group B and the control group (P＞0.05). According to the results of immunohistochemistry, Wnt5a expression in group A (P＜0.01) and group B (P＜0.05) was significantly higher than that in normal controls. Wnt5a expression in group A was higher than that in group B (P＜0.05). Conclusion: Wnt5a expression is associated with the bone resorption in chronic apical periodontitis.

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