口腔医学研究

口腔医学研究 ›› 2016, Vol. 32 ›› Issue (8): 857-860.DOI: 10.13701/j.cnki.kqyxyj.2016.08.020

• 临床研究论著 • 上一篇    下一篇

E-cadherin和Vimentin在OSCC上皮-间质转化中的作用及表达

陈震1,郭燕2*   

  1. 1. 上海市普陀区人民医院口腔科 上海 200060;
    2. 中国医科大学第四医院口腔科 辽宁 沈阳 110000
  • 收稿日期:2015-10-19 出版日期:2016-08-26 发布日期:2016-08-26
  • 通讯作者: 郭燕,E-mail:3219475665@qq.com
  • 作者简介:陈震(1976~ ),安徽人,女,硕士,主治医师,主要从事口腔修复的临床治疗工作。
  • 基金资助:
    辽宁省自然科学基金(编号:2013021094)

Expression and Roles of E-cadherin and Vimentin in Epithelium-mesenchymal Transition of Oral Squamous Cell Carcinoma

CHEN Zheng1, GUO Yan2*   

  1. 1. Putuo People's Hospital, Shanghai 200060, China;
    2. Affiliated Fourth Hospital of China Medical University, Shenyang 110000, China
  • Received:2015-10-19 Online:2016-08-26 Published:2016-08-26

摘要： 目的:探讨E-钙黏蛋白(E-cadherin)和波形蛋白(Vimentin)在口腔鳞状细胞癌(OSCC)上皮-间质转化中的作用及表达。方法:选取76例口腔鳞状细胞癌(OSCC)患者和40例健康者作为对照组,利用RT-PCR检测OSCC和正常口腔黏膜组织中TGF-β1、E-cadherin和Vimentin表达。结果:OSCC患者组织中TGF-β1 mRNA和Vimentin mRNA相对表达量均高于对照组,而E-cadherin mRNA相对表达量低于对照组,差异均有统计学意义(P<0.05);TGF-β1、E-cadherin和Vimentin在OSCC组织中表达均与分化程度有关,分化程度越高,TGF-β1 mRNA和Vimentin mRNA相对表达量越高,而E-cadherin mRNA相对表达量则越低;Pearson相关分析显示,OSCC中TGF-β1 mRNA相对表达量与E-cadherin mRNA相对表达量呈负相关(r=-0.322,P=0.004),而与Vimentin mRNA相对表达量呈正相关(r=0.661,P=0.000)。结论:TGF-β1和Vimentin 在OSCC组织中表达增强,而E-cadherin则表达降低,提示EMT过程在OSCC发生及进展过程中发挥重要作用。

关键词: 口腔鳞状细胞癌, E-钙黏蛋白, 波形蛋白, TGF-β1

Abstract: Objective: To investigate the expression and roles of E-cadherin and vimentin in epithelium- mesenchymal transition (EMT) of oral squamous cell carcinoma (OSCC). Methods: Seventy-six cases with OSCC and 40 healthy subjects as control group were involved. The expression of TGF-β1, E-cadherin and Vimentin were detected by RT-PCR. Results: The gene expression levels of TGF-β1 and Vimentin in the tissues of OSCC patients were significantly higher than healthy subjects, while the gene expression levels of E-cadherin was significantly lower (P<0.05). The expression levels of TGF-β1, E-cadherin and Vimentin were associated with the degree of cell differentiation in OSCC tissues. Pearson correlation analysis showed that the gene expression levels of TGF-β1 was negatively correlated with E-cadherin in OSCC (r=-0.322, P=0.004), but was positively correlated with Vimentin (r=0.661, P=0.000). Conclusion: The expression of TGF-β1 and Vimentin was up-regulated in OSCC, whereas the expression of E-cadherin was down-regulated, indicating that EMT plays an important role in the development of OSCC.

Key words: Oral squamous cell carcinoma, E-cadherin, Vimentin, TGF-β1

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