大鼠正畸牙移动中NF-κB通路相关差异基因的GO分析

doi:10.13701/j.cnki.kqyxyj.2017.08.008

口腔医学研究 ›› 2017, Vol. 33 ›› Issue (8): 832-836.DOI: 10.13701/j.cnki.kqyxyj.2017.08.008

大鼠正畸牙移动中NF-κB通路相关差异基因的GO分析

李猷¹, 张淋坤^2*, 张锡忠², 康永歌¹, 马遥¹

1. 南开大学医学院天津 300071;
2. 天津市口腔医院正畸科天津 300041

收稿日期:2017-01-05 出版日期:2017-08-20 发布日期:2017-08-28
通讯作者: 张淋坤,E－mail:zlkxjtu@163.com
作者简介:李猷(1990~ ),男,硕士在读,医师,主要从事口腔正畸学的临床研究工作。
基金资助:
国家临床重点专科建设项目(口腔正畸专业)批准文号:国卫医办函[2013]544号 ;天津市卫生计生委科技基金重点攻关项目(编号:14KG132)

GO Analysis of Differentially Expressed Genes of NF- kappa B Pathway in Rat Orthodontic Tooth Movement.

LI You¹, ZHANG LIN-kun^2*, ZHANG XI-zhong², KANG YONG-ge¹, MA Yao¹.

1. Medical College of Nankai University, Tianjin 300071, China;
2. Department of Stomotology, Tianjin Stomotological Hospital, Tianjin 300041, China.

Received:2017-01-05 Online:2017-08-20 Published:2017-08-28

摘要/Abstract

摘要： 目的:利用基因表达谱芯片对正畸加力组与正常大鼠牙齿牙周组织的基因表达谱进行对比分析,筛选差异基因并进行GO分析。方法:将6只SD大鼠随机分为加力组和对照组,加力组建立大鼠正畸牙齿移动模型。根据预实验结果,分别在0 h、12 h处死大鼠,并截取上颌第一磨牙及外周约1 mm的牙周组织,提取总RNA,进行全基因组表达谱芯片检测,对差异表达基因进行GO分析,并运用RT-qPCR对芯片结果进行验证。结果:芯片结果显示,共筛选到463条差异基因,其中实验组与对照组相比250个基因表达上调,213个基因表达下调,在差异基因中涉及到NF-κB信号通路的基因有8个,均表达上调。GO分析涉及到1189个不同的功能分类。结论:正畸加力12h后牙齿牙周组织中与炎症反应相关的基因和与中性粒细胞趋化相关的基因表达上调。

关键词: 正畸牙移动, 核转录因子κB, 炎症反应, 中性粒细胞, 趋化

Abstract: Objective: To compare the mRNA expression profiles of periodontal tissues surrounding tooth between normal rats and rats with orthodontic force, to screen different genes and perform GO analysis by microarray technology. Methods: Six SD rats were randomly selected and divided into experimental group and control group. The experimental group established rat orthodontic tooth movement model. According to the results of pre-experiment, the rats were sacrificed at 0h and 12h, respectively, and the maxillary first molar peripheral 1 mm periodontal tissues were harvested and sampled, then total RNA was extracted for rat whole genome microarray detection. GO analysis of differentially expressed genes was performed and RT-qPCR was used to confirm the results on core samples. Results: Microarray results showed that, in contrast to control group, a total of 463 differentially expressed genes, including 250 up-regulated genes and 213 down-regulated genes existed in the experimental group. Among the differentially expressed genes, there were 8 up-regulated genes involved in NF- kappa B signaling pathway. And the GO analysis involved 1189 different functional categories. Conclusion: Inflammation and neutrophil chemotaxis related genes up-regulated in periodontal tissue during orthodontics tooth movement.

Key words: Orthodontics tooth movement , Nuclear transcription factor-κB , Inflammation , Neutrophil , Chemotaxis

中图分类号:

R783.5

李猷, 张淋坤, 张锡忠, 康永歌, 马遥. 大鼠正畸牙移动中NF-κB通路相关差异基因的GO分析[J]. 口腔医学研究, 2017, 33(8): 832-836.

LI You, ZHANG LIN-kun, ZHANG XI-zhong, KANG YONG-ge, MA Yao.. GO Analysis of Differentially Expressed Genes of NF- kappa B Pathway in Rat Orthodontic Tooth Movement.[J]. Journal of Oral Science Research, 2017, 33(8): 832-836.

参考文献

[1] Karin M, Greten FR. NF-kappaB: linking inflammation and immunity to cancer development and progression [J]. Nature Reviews Immunology, 2005, 5(10)∶749-759
[2] 孙大业,崔素娟,孙颖.细胞信号转导[M].北京:科学出版社,2010∶275
[3] 黄艳, 王旭霞, 张君,等. 正畸牙移动实验动物模型的建立[J].口腔医学,2007,27(2)∶64-66
[4] 卢建,余应年,徐仁宝.受体、信号转导系统与疾病[M].济南:山东科学技术出版社,1999,338∶560-563
[5] Bildt M M, Henneman S, Maltha J C, et al. CMT-3 inhibits orthodontic tooth displacement in the rat [J]. Archives of Oral Biology, 2007, 52(6)∶571-578
[6] 王庆,郭杰.低能量激光用于正畸治疗的研究进展[J].广东牙病防治,2013,21(3)∶158-162
[7] T P Kasten, P Collin-Osdoby, N Patel, et al. Potentiation of osteoclast bone-resorption activity by inhibition of nitric oxide synthase [J]. Proceedings of the National Academy of Sciences of the United States of America, 1994, 91(9)∶3569-3573
[8] Schalkwijk CG, De VE, Pfeilschifter J, et al. Interleukin-1 beta and transforming growth factor-beta 2 enhance cytosolic high-molecular-mass phospholipase A2 activity and induce prostaglandin E2 formation in rat mesangial cells [J]. European Journal of Biochemistry, 1992, 210(1)∶169-176
[9] Pfeilschifter J, Schalkwijk C, Briner VA, et al. Cytokine-stimulated secretion of group II phospholipase A2 by rat mesangial cells. Its contribution to arachidonic acid release and prostaglandin synthesis by cultured rat glomerular cells [J]. Journal of Clinical Investigation, 1993, 92(5)∶2516-2523
[10] Bird TA, Kyriakis JM, Tyshler L, et al. Interleukin-1 activates p54 mitogen-activated protein (MAP) kinase/stress-activated protein kinase by a pathway that is independent of p21ras, Raf-1, and MAP kinase kinase [J]. Journal of Biological Chemistry, 1994, 269(50)∶31836-31844
[11] 李晓玫,李彪.白细胞介素1细胞信号转导机制研究现状[J].生理科学进展,1998(1)∶59-62
[12] 丁宁,肖慧,许立新,等.机械牵张诱导肺泡巨噬细胞的细胞因子表达谱及其与脂多糖对MIP-2释放的协同效应[J].中国呼吸与危重监护杂志,2009,8(2)∶166-170
[13] Burke SJ, Lu D, Sparer TE, et al. NF-κB and STAT1 control CXCL1 and CXCL2 gene transcription [J]. American Journal of Physiology Endocrinology & Metabolism, 2014, 306(2)∶E131-149
[14] 梁法生,宋继昌,高英堂,等.巨噬细胞炎性蛋白-2在大鼠原位肝移植后的表达[J].中华器官移植杂志,2001,22(2)∶91-93
[15] Furie M B, Randolph G J. Chemokines and tissue injury [J]. American Journal of Pathology, 1995, 146(6)∶1287-1301
[16] Zhang F, Warskulat U, Wettstein M, et al. Hyperosmolarity stimulates prostaglandin synthesis and cyclooxygenase-2 expression in activated rat liver macrophages [J]. Biochemical Journal, 1995, 312(1)∶135-143
[17] 王建春,毛宝龄.环氧合酶2研究进展[J].临床与病理杂志, 1998(1)∶30-33

大鼠正畸牙移动中NF-κB通路相关差异基因的GO分析

GO Analysis of Differentially Expressed Genes of NF- kappa B Pathway in Rat Orthodontic Tooth Movement.

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