间充质干细胞表面标记在颞下颌关节疾病患者滑膜间充质细胞表达的比较

doi:10.13701/j.cnki.kqyxyj.2018.11.024

口腔医学研究 ›› 2018, Vol. 34 ›› Issue (11): 1249-1252.DOI: 10.13701/j.cnki.kqyxyj.2018.11.024

间充质干细胞表面标记在颞下颌关节疾病患者滑膜间充质细胞表达的比较

路红艳¹, 李健², 龙星^2*

1. 长沙市口腔医院颌面外科湖南长沙 410000;
2. 武汉大学口腔医院第二口腔颌面外科湖北武汉 430079

收稿日期:2018-06-25 出版日期:2018-11-28 发布日期:2018-11-23
通讯作者: 龙星,E-mail: longxing@whu.edu.cn
作者简介:路红艳(1985~ ),女,山东人,硕士,主治医师,主要从事牙槽外科及颞下颌关节病研究。
基金资助:
国家自然科学基金(编号:81600889、8177041189)

Comparison of Expression of Mesenchymal Stem Cell Surface Markers on Synovial Mesenchymal Cells of Patients with Temporomandibular Joint Disease

LU Hong-yan¹, LI Jian², LONG Xing^2*

1. Department of Oral and Maxillofacial Surgery, Changsha Stomatology Hospital, Changsha 410000, China;
2. Department of 2nd Oral and Maxillofacial Surgery, School of Stomatology, Wuhan University, Wuhan 430079, China.

Received:2018-06-25 Online:2018-11-28 Published:2018-11-23

摘要/Abstract

摘要： 目的:检测间充质干细胞表面标记(CD73、 CD90、CD105、CD271和CD34)在人颞下颌关节滑膜间充质细胞(synovial mesenchymal cells,SMCs)上的表达;同时比较这些标记在髁突肥大滑膜无病变和颞下颌关节骨关节病伴关节盘穿孔患者SMCs上表达水平的差异。方法:分别采用多次贴壁培养和组织块法分离得到髂骨骨髓间充质干细胞(mesenchymal stem cells,MSCs)和颞下颌关节原代SMCs并传至3代,应用流式细胞仪检测CD34、CD73、 CD90、CD105、CD271的表达水平。结果:髂骨MSCs及颞下颌关节SMCs表面CD73、CD90、CD105表达量均>90%,CD271表达量<1% ,CD34表达量较低为0.47%~30.00%。结论:髂骨MSCs和颞下颌关节SMCs高表达CD73、CD90、CD105,CD271和CD34表达量较低;CD34在人颞下颌关节骨关节病患者SMCs上的表达量较髁突肥大滑膜无病变患者高。

关键词: 骨髓间充质干细胞, 颞下颌关节滑膜间充质细胞, 间充质干细胞表面标记, 颞下颌关节骨关节病

Abstract: Objective: To detect whether mesenchymal stem cells (MSCs) markers (CD34, CD73, CD90, CD105, CD271) were expressed in synovial mesenchymal cells(SMCs) of temporomandibular joint and to compare their expression in SMCs of temporomandibular joint from human osteoarthritisis and condylar hypertrophy. Methods: SMCs of temporomandibular joint were isolated using explants culture method and MSCs were obtained by the repeated adherent culture method. Then, the third generation cells were characterized and the expression of CD73, CD90, CD105, CD271, and CD34 were qualified by flow cytometry. Results: Fluorescence-activated cell sorting (FACS) analysis showed more than 90% MSCs from human iliac bone and SMCs from human temporomandibular joint expressed CD73, CD90, and CD105, less than 1% cells expressed CD271, and the quantification of CD34 was 0.47%-30%. Conclusion: MSCs from human iliac bone and SMCs of temporomandibular joint from human were positive for CD73, CD90, and CD105, while the expression of CD34 and CD271 were low. SMCs of temporomandibular joint with osteoarthritisis contained more cells positive for CD34 antigens than those from temporomandibular joint condylar hypertrophy.

Key words: Bone marrow mesenchymal stem cells, Synovial mesenchymal cells of temporomandibular joint, Mesenchymal stem cell surface markers, Osteoarthritisis of temporomandibular joint

路红艳, 李健, 龙星. 间充质干细胞表面标记在颞下颌关节疾病患者滑膜间充质细胞表达的比较[J]. 口腔医学研究, 2018, 34(11): 1249-1252.

LU Hong-yan, LI Jian, LONG Xing. Comparison of Expression of Mesenchymal Stem Cell Surface Markers on Synovial Mesenchymal Cells of Patients with Temporomandibular Joint Disease[J]. Journal of Oral Science Research, 2018, 34(11): 1249-1252.

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间充质干细胞表面标记在颞下颌关节疾病患者滑膜间充质细胞表达的比较

Comparison of Expression of Mesenchymal Stem Cell Surface Markers on Synovial Mesenchymal Cells of Patients with Temporomandibular Joint Disease

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