脂多糖促进牙周膜间充质干细胞组蛋白去甲基化酶HR表达的研究

doi:10.13701/j.cnki.kqyxyj.2018.06.018

口腔医学研究 ›› 2018, Vol. 34 ›› Issue (6): 648-652.DOI: 10.13701/j.cnki.kqyxyj.2018.06.018

脂多糖促进牙周膜间充质干细胞组蛋白去甲基化酶HR表达的研究

张琛¹, 姚睿^2*, 范志朋^3*

1. 天津医科大学天津 300070;
2. 天津市口腔医院儿童口腔科天津 300041;
3. 首都医科大学口腔医学院口腔医学研究所全牙再生及口腔组织功能重建北京市重点实验室北京 100050

收稿日期:2017-10-18 出版日期:2018-06-20 发布日期:2018-06-21
通讯作者: 姚睿,E-mail: yaorui73@163.com; 范志朋,E-mail: wang54384@163.com
作者简介:张琛(1991~ ),女,河北省沧州市人,硕士研究生,主要从事口腔内科临床及细胞分子生物学研究。
基金资助:
北京市自然科学基金(编号:7172089)

Lipopolysaccharide Enhanced the Expressions of HR in Periodontal Ligment Stem Cells

ZHANG Chen¹, YAO Rui^2*, FAN Zhi-peng^3*

1. Tianjin Medical University School, Tianjin, 300070, China;
2. Department of Pediatric Dentistry, Stomatological Hospital of Tianjin, 300041, China;
3. Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction,School of Stomatology,Capital Medical University,Beijing,100050,China

Received:2017-10-18 Online:2018-06-20 Published:2018-06-21

摘要/Abstract

摘要： 目的:脂多糖是否对牙周膜间充质干细胞(periodontal ligment stem cells, PDLSCs)中组蛋白去甲基化酶相关基因表达存在调控功能。方法:利用不同剂量的脂多糖对牙周膜干细胞进行刺激处理;在mRNA水平利用实时定量RT-PCR (real time reverse transcription-polymerase chain reaction,qRT-PCR)检测组蛋白去甲基化酶相关基因的表达。qRT-PCR结果显示HR(hairless homolog)基因在脂多糖刺激后24 h和48 h结果显示,能促进HR的表达,其表达升高的水平与脂多糖刺激时间有相关性。1、5和10 mg/L脂多糖在作用48 h后都能促进HR的表达,其表达升高的水平与脂多糖刺激剂量有相关性。10 μg/mL脂多糖抑制牙周膜干细胞体外矿化能力。结论:在牙周膜干细胞中脂多糖对牙周膜干细胞组蛋白去甲基化酶HR的表达有促进作用。

关键词: 脂多糖, 组蛋白去甲基化酶, 牙周膜干细胞, Hairless, 骨向分化

Abstract: Objective: To investigate whether LPS could regulate the expression of histone demethylases in periodontal ligament stem cells (PDLSCs). Methods: Different doses of LPS were used to stimulate the PDLSCs. The qRT-PCR was used to detect the expression of histone demethylases at the mRNA level. Results: The qRT-PCR results showed that the expression levels of HR were up-regulated at 24 and 48h after LPS treatment in a time dependent manner. And 1, 5, and 10 μg/mL LPS could increase the expression level of HR in a dose dependent manner at 48h. 10 μg/mL LPS inhibited the mineralization of PDLSCs. Conclusion: LPS could induce the expression level of histone demethylase HR in PDLSCs.

Key words: Lipopolysaccharide, Histone demethylase, Periodontal ligament stem cells, Hairless, Osteogenic differentiation

张琛, 姚睿, 范志朋. 脂多糖促进牙周膜间充质干细胞组蛋白去甲基化酶HR表达的研究[J]. 口腔医学研究, 2018, 34(6): 648-652.

ZHANG Chen, YAO Rui, FAN Zhi-peng. Lipopolysaccharide Enhanced the Expressions of HR in Periodontal Ligment Stem Cells[J]. Journal of Oral Science Research, 2018, 34(6): 648-652.

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脂多糖促进牙周膜间充质干细胞组蛋白去甲基化酶HR表达的研究

Lipopolysaccharide Enhanced the Expressions of HR in Periodontal Ligment Stem Cells

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