氢氧化钙对人根尖牙乳头干细胞增殖的影响

doi:10.13701/j.cnki.kqyxyj.2018.09.016

口腔医学研究 ›› 2018, Vol. 34 ›› Issue (9): 987-990.DOI: 10.13701/j.cnki.kqyxyj.2018.09.016

氢氧化钙对人根尖牙乳头干细胞增殖的影响

杨维虹,谢茹,刘瑶,刘兴容^*

西南医科大学附属口腔医院儿童口腔科四川泸州 646000

收稿日期:2018-03-20 出版日期:2018-09-28 发布日期:2018-09-25
通讯作者: 刘兴容,E-mail:liuxingrong163@163.com
作者简介:杨维虹(1992~ ),女,四川人,硕士在读,主要从事牙体牙髓病的基础与临床研究。

Effects of Calcium Hydroxide on Stem Cells Proliferation from Apical Papilla of Human.

YANG Wei-hong, XIE Ru, LIU Yao, LIU Xing-rong^*.

Department of Pediatric Dentistry, the Affiliated Stomatology Hospital of Southwest Medical University, Luzhou 646000, China.

Received:2018-03-20 Online:2018-09-28 Published:2018-09-25

摘要/Abstract

摘要： 目的: 探讨氢氧化钙(calcium hydroxide,CH)对人根尖牙乳头干细胞(stem cells from apical papilla,SCAPs)增殖能力的影响。方法: 运用酶消化法结合组织块贴壁法分离培养SCAPs并加以鉴定。根据CH浸提原液的浓度不同分为6个实验组,在培养后的第1、3、5、7、9天分别终止培养,利用MTT法检测其吸光度(A)值,并进行统计学分析。结果: 与对照组比较,至第5天时,浓度为0.1%、0.5%、1.0%、5.0%组的SCAPs增殖比较差异无统计学意义(P>0.05),而浓度为10.0%和15.0%组已表现出对SCAPs增殖的抑制作用(P<0.05);到第7天和第9天时,所有实验组与对照组比较,均表现为对SCAPs的增殖抑制作用(P<0.05)。结论: CH对人SCAPs的增殖起抑制作用,并且随着时间延长其抑制作用增强,而高浓度CH对人SCAPs增殖的抑制作用更为明显。

关键词: 根尖牙乳头干细胞, 氢氧化钙, 培养, 鉴定, 增殖

Abstract: Objective: To investigate the effect of calcium hydroxide (CH) on proliferation of stem cells from apical papilla (SCAPs) of young human. Methods: The human SCAPs were isolated and cultivated by enzyme digestion method combined with adherent explanted tissue culture. According to the concentration of CH extract, it is divided into six experimental groups. The cultivation was terminated after 1, 3, 5, 7, and 9 days. The proliferation was determined by MTT method. Results: Compared with the control group, the proliferation of SCAPs in the 0.1%, 0.5%, 1.0%, and 5.0% groups was not significantly different at day 5 (P>0.05), however, the proliferation in the 10.0% and 15.0% groups was inhibited (P<0.05). At day 7 and 9, the proliferation of all experimental groups was inhibited (P<0.05). Conclusition: CH inhibits the proliferation of human SCAPs, which is enhanced with time elapse. The inhibitory effect of CH on human SCAPs proliferation is more obvious at high concentration.

Key words: Stem cells from the apical papilla , Calcium hydroxide , Cell culture, Identification, Proliferation

杨维虹,谢茹,刘瑶,刘兴容. 氢氧化钙对人根尖牙乳头干细胞增殖的影响[J]. 口腔医学研究, 2018, 34(9): 987-990.

YANG Wei-hong, XIE Ru, LIU Yao, LIU Xing-rong.. Effects of Calcium Hydroxide on Stem Cells Proliferation from Apical Papilla of Human.[J]. Journal of Oral Science Research, 2018, 34(9): 987-990.

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氢氧化钙对人根尖牙乳头干细胞增殖的影响

Effects of Calcium Hydroxide on Stem Cells Proliferation from Apical Papilla of Human.

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