D-组氨酸抑制变异链球菌生物膜作用的研究

doi:10.13701/j.cnki.kqyxyj.2019.02.018

口腔医学研究 ›› 2019, Vol. 35 ›› Issue (2): 176-179.DOI: 10.13701/j.cnki.kqyxyj.2019.02.018

D-组氨酸抑制变异链球菌生物膜作用的研究

全旭^1,2,许彤彤^1,2,张慧彦^1,2,王鹤龄^1,2,王春阳^1,2,崔云霞^1,2,付齐月^1,2,孟维艳^1*

1.吉林大学口腔医院口腔种植中心吉林长春 130000;
2. 吉林省牙发育及颌骨重塑与再生重点实验室吉林长春 130000

收稿日期:2018-08-30 出版日期:2019-02-18 发布日期:2019-02-25
通讯作者: 孟维艳,E-mail:mengsitong66@163.com
作者简介:全旭(1991~ ),女,辽宁人,硕士在读,研究方向为种植体周围炎及其相关药物。
基金资助:
国家自然科学基金(编号:81571007) 吉林省科技发展自然科学基金(编号:20180101121JC)

Effects of D-Histidine on Streptococcus Mutans Biofilm.

QUAN Xu^1,2, XU Tong-tong^1,2, ZHANG Hui-yan^1,2, WANG He-ling^1,2, WANG Chun-yang^1,2, CUI Yun-xia^1,2, FU Qi-yue^1,2, MENG Wei-yan^1*

1. Department of Dental Implantology, School and Hospital of Stomatology, Changchun 130021, China;
2. Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling, Jinlin University, Changchun 130021, China.

Received:2018-08-30 Online:2019-02-18 Published:2019-02-25

摘要/Abstract

摘要： 目的: 研究D-组氨酸对变异链球菌生物膜的抑制作用。方法: 通过生长曲线实验评估D-组氨酸对变异链球菌细菌活性的影响。通过结晶紫染色实验评估变异链球菌形成生物膜的量;采用胞外多糖实验检测D-组氨酸对变异链球菌生物膜胞外多糖含量的影响;经扫描电镜观察经D-组氨酸处理后变异链球菌生物膜的结构。结果: 经生长曲线实验评估发现D-组氨酸在浓度≥20%时抑制变异链球菌的生物活性且呈浓度依赖性。通过结晶紫染色实验和生物膜胞外多糖实验评估发现,D-组氨酸浓度≥40%时抑制变异链球菌生物膜的形成并影响生物膜胞外多糖的含量。经扫描电镜观察,40%的D-组氨酸即可影响变异链球菌生物膜的形成,同时细菌的粘附量有所下降。结论: D-组氨酸具有良好的抑制变异链球生物膜的作用,并影响变异链球菌的细菌活性。

关键词: 生物膜, 变异链球菌, D-组氨酸

Abstract: Objective s: To evaluate the effects of D-Histidine on the Streptococcus mutans biofilm in vitro. Methods: Antimicrobial activity testing of D-Histidine was evaluated by determining the growth curve. Crystal violet biofilm biomass assay and polysaccharide measurement were performed to evaluate the potential activity of D-Histidine effectively inhibiting biofilm formation. SEM was used to observe the morphology and the structure of biofilm. Results: Growth curve showed that D-Histidine at concentration of 20% interfered with the viability of Streptococcus mutans in a dose-dependent manner. CV and EPS assays showed that D-Histidine at concentration of 40% effectively inhibited Streptococcus mutans biofilm formation and the polysaccharide level of Streptococcus mutans biofilm was greatly reduced. SEM results displayed that D-Histidine effectively inhibited Streptococcus mutans bioflim formation at concentration of 40% and bacterial aggregation amount declined with increasing concentrations of D-Histidine. Conclusion: D-Histidine effectively inhibits Streptococcus mutan biofilm formation in a dose-dependent manner and D-Histidine interferes with the viability of Streptococcus mutans.

Key words: Biofilm, Streptococcus mutans, D-Histidine

全旭,许彤彤,张慧彦,王鹤龄,王春阳,崔云霞,付齐月,孟维艳. D-组氨酸抑制变异链球菌生物膜作用的研究[J]. 口腔医学研究, 2019, 35(2): 176-179.

QUAN Xu, XU Tong-tong, ZHANG Hui-yan, WANG He-ling, WANG Chun-yang, CUI Yun-xia, FU Qi-yue, MENG Wei-yan.. Effects of D-Histidine on Streptococcus Mutans Biofilm.[J]. Journal of Oral Science Research, 2019, 35(2): 176-179.

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D-组氨酸抑制变异链球菌生物膜作用的研究

Effects of D-Histidine on Streptococcus Mutans Biofilm.

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