饰胶蛋白聚糖与口腔鳞状细胞癌发生发展的相关性研究

doi:10.13701/j.cnki.kqyxyj.2020.05.009

口腔医学研究 ›› 2020, Vol. 36 ›› Issue (5): 437-442.DOI: 10.13701/j.cnki.kqyxyj.2020.05.009

饰胶蛋白聚糖与口腔鳞状细胞癌发生发展的相关性研究

赖永先¹, 聂敏海¹, 陈潇², 刘旭倩^1*

1.西南医科大学附属口腔医院牙周黏膜病科;西南医科大学口颌面修复重建和再生实验室四川泸州 646000;
2.四川绵阳口腔医院正畸科四川绵阳 621000

收稿日期:2020-01-06 出版日期:2020-06-16 发布日期:2020-06-18
通讯作者: ^* 刘旭倩,E-mail:liuxuqiankokky@126.com
作者简介:赖永先(1988~ ),女,四川泸州人,硕士在读,医师,研究方向:口腔黏膜。
基金资助:
四川省教育厅科研项目(编号:18ZA0523);四川省医学科研课题计划(编号:Q18032);四川省科技厅科技计划(编号:2018JY0401)

Correlation between Decorin and Oral Squamous Cell Carcinoma

LAI Yongxian¹, NIE Minhai¹, CHEN Xiao², LIU Xuqian^1*

1. Department of Periodontics & Oral Mucosal Diseases, The Affiliated Stomatology Hospital of Southwest Medical University. Luzhou 646000, China;
2. Department of Orthodontics, Mianyang Stomatological Hospital. Mianyang 621000, China

Received:2020-01-06 Online:2020-06-16 Published:2020-06-18

摘要/Abstract

摘要： 目的:探讨饰胶蛋白聚糖(Decorin, DCN)在口腔鳞状细胞癌(oral squamous cell carcinoma, OSCC)中的表达和意义,及DCN影响OSCC发生发展的可能机制。方法:采用免疫组织化学法检测DCN、表皮生长因子受体(epidermal growth factor receptor, EGFR)、致癌基因C-Myc和细胞周期蛋白依赖性激酶抑制剂(cyclin dependent kinase inhibitor, P21)四种因子在OSCC组织和正常口腔黏膜组织中的表达;用脂质体法将DCN质粒转入HSC-3细胞中,采用Real time PCR和Western blot检测DCN过表达对EGFR、C-Myc和P21表达的影响。结果:随着OSCC分化程度的降低,DCN蛋白和P21蛋白的阳性表达率逐渐降低,EGFR蛋白和C-Myc蛋白的阳性表达率逐渐升高(P<0.05);与空载体组和对照组相比,转染组EGFR和C-Myc mRNA表达量和蛋白表达水平皆降低,P21 mRNA表达量和蛋白表达水平皆升高(P<0.05)。结论:DCN与OSCC的发生、发展过程紧密相关,其对OSCC的作用可能通过调节EGFR、C-Myc和P21实现。

关键词: 鳞状细胞癌, 饰胶蛋白聚糖, 表皮生长因子受体, 致癌基因, 细胞周期蛋白依赖性激酶抑制剂

Abstract: Objective: To investigate the expression and significance of Decorin (DCN) in oral squamous cell carcinoma (OSCC), and the possible mechanism by which DCN affects the development of OSCC. Methods: The expressions of the DCN, epidermal growth factor receptor (EGFR), oncogene C-Myc, and cyclin dependent kinase inhibitor (P21) in OSCC tissues and normal gingiva tissues were detected by immunohistochemistry. After being transfected with DCN plasmid, the expressions of EGFR, C-Myc, and P21 in HSC-3 cells were detected by Real time PCR and Western blot. Results: With the decreasing of differentiation degree of OSCC, the positive expressions of DCN and P21 proteins were decreased gradually, while the EGFR and C-Myc proteins were increased gradually (P<0.05). Compared with HK group and control group, the mRNA and protein levels of EGFR and C-Myc in transfection group were decreased, while the mRNA and protein level of P21 was increased (P<0.05). Conclusion: DCN is related to the occurrence and development of OSCC, which might reach the goal by regulating the expressions of EGFR, C-Myc, and P21.

Key words: squamous cell carcinoma, DSPG-Ⅱ, epidermal growth factor receptor, C-myc proteins, cyclin kinase inhibitor p21

赖永先, 聂敏海, 陈潇, 刘旭倩. 饰胶蛋白聚糖与口腔鳞状细胞癌发生发展的相关性研究[J]. 口腔医学研究, 2020, 36(5): 437-442.

LAI Yongxian, NIE Minhai, CHEN Xiao, LIU Xuqian. Correlation between Decorin and Oral Squamous Cell Carcinoma[J]. Journal of Oral Science Research, 2020, 36(5): 437-442.

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饰胶蛋白聚糖与口腔鳞状细胞癌发生发展的相关性研究

Correlation between Decorin and Oral Squamous Cell Carcinoma

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