白细胞介素-17介导牙髓基质金属蛋白酶表达和调节牙髓炎症的机制初探

doi:10.13701/j.cnki.kqyxyj.2021.05.010

摘要/Abstract

摘要： 目的:探究白细胞介素(IL)-17在牙髓炎症过程中时空表达,研究IL-17对牙髓炎可能的调节机制。方法:构建大鼠牙髓炎模型,苏木精-伊红(HE)染色观察牙髓组织结构形态,免疫组织化学染色检测牙髓中IL-17表达;培养人牙髓细胞,肿瘤细胞坏死因子(TNF)-α刺激模拟炎症状态,RT-qPCR检测IL-17基因表达,ELISA检测IL-17的蛋白表达; IL-17重组蛋白刺激牙髓细胞,RT-qPCR检测MMP-1、MMP-2、MMP-3和MMP-13的表达,胶原酶谱和明胶酶谱检测MMP-1和MMP-2酶活性;Western blot检测信号通路表达。结果:IL-17在牙髓炎症过程中持续高表达,体外TNF-α刺激可升高牙髓细胞IL-17的表达。IL-17刺激牙髓细胞,上调MMP-1和MMP-2的酶活性,并激活丝裂原蛋白激酶(MAPK)信号通路。结论:IL-17在炎症牙髓表达上调,并可通过升高MMP-1和MMP-2的表达,激活MAPK信号通路发挥促炎作用。

关键词: 牙髓炎, 白细胞介素-17, 基质金属蛋白酶, 免疫学

Abstract: Objective: To investigate the role of interleukin-17 in dental pulp inflammation and the relationship between IL-17 and MMPs. Methods: Immunohistochemical staining was used to detect the expression of TNF-α and IL-17 in pulp tissues. TNF-α or IL-17 recombinant protein was used to treat human dental pulp cells. RT-qPCR, Western blotting, and ELISA were used to detect the expression of mRNA and protein levels of IL-17, MMP-1, MMP-2, MMP-3 and MMP-13. Gelatin zymography and collagen zymography were used to detect the enzymatic activity of MMP-1 and MMP-2. Results: IL-17 was high-expressed during pulpitis. TNF-α treatment could induce expression of IL-17 in HDPCs. IL-17 mainly contributed to its positive regulatory role in inflammation through up regulating MMPs and activating protein kinase MAPK pathway. Conclusion: IL-17 acts as an inflammatory mediator in dental pulp inflammation by acting MAPK pathway. IL-17 can induce the expression of MMP-1 and MMP-2 in HDPCs

Key words: pulpitis, interleukin-17, matrix metalloproteinase, immunology

刘梦余. 白细胞介素-17介导牙髓基质金属蛋白酶表达和调节牙髓炎症的机制初探[J]. 口腔医学研究, 2021, 37(5): 425-430.

LIU Mengyu. Mechanism of Interleukin-17 Regulating Matrix Metalloproteinase Expression and Inflammation in Dental Pulp[J]. Journal of Oral Science Research, 2021, 37(5): 425-430.

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