牙周炎对高脂饮食小鼠血浆氧化三甲胺的影响

doi:10.13701/j.cnki.kqyxyj.2023.01.014

摘要/Abstract

摘要： 目的:评估牙周炎对高脂饮食小鼠血浆氧化三甲胺(trimethylamine N-oxide, TMAO)生成的影响并探索肠道菌群在其中的作用。方法:选取10只雄性C57BL/6J小鼠随机分为高脂饮食组(HFD)和高脂饮食+牙周炎组(HFD_PD)。实验8周后安乐处死小鼠,检测牙槽骨丢失量,血浆TMAO代谢组浓度,肝脏和肠道相关基因表达,盲肠内容物微生物组成。结果:与HFD组相比,HFD_PD组小鼠血浆TMAO浓度增加(P<0.01);门水平上,HFD_PD组小鼠Firmicutes和Desulfobacterota的丰度降低,Bacteroidota和Campilobacterota的丰度升高;Pearson相关性分析显示,Eubacterium_coprostanoligenes,Lachnospiraceae_NK4A136与TMAO呈正相关,Muribaculaceae与TMAO呈负相关(P<0.05);肠道组织IL-6的基因表达量升高(P<0.01),ZO-1的表达量下降(P<0.01);肝脏IL-6和TNF-α基因表达量升高(P<0.01,P<0.001)。结论:牙周炎可增加高脂饮食小鼠血浆TMAO的浓度,可能是通过调节与TMAO代谢相关的肠道菌群而实现的。

关键词: 牙周炎, 氧化三甲胺, 肠道菌群

Abstract: Objective: To evaluate the effects of periodontitis on plasma trimethylamine N-oxide (TMAO) production in mice fed a high-fat diet and to explore the role of gut microbiota. Methods: Ten wild-type male C57BL/6J mice were randomly divided into 2 groups: (1) high-fat diet group (HFD) and (2) high-fat diet + periodontitis group (HFD_PD). Eight weeks later, the mice were sacrificed to detect the alveolar bone loss, the concentration of plasma TMAO, the relative gene expression in liver and gut, and microbial communities in cecal contents. Results: Compared with HFD group, the concentration of plasma TMAO was significantly increased in HFD_PD group (P<0.01). At the phylum level, the abundance of Firmicutes and Desulfobacterota decreased and the abundance of Bacteroidota and Campilobacterota increased (P<0.05). Pearson correlation analysis showed that Eubacterium_coprostanoligenes and Lachnospiraceae_NK4A136 in HFD_PD group were positively correlated with plasma TMAO levels, while Muribaculaceae was negatively correlated with plasma TMAO levels (P<0.05). The expression of IL-6 in intestinal tissue was increased (P<0.01), while the expression of ZO-1 was decreased (P<0.01). The expression levels of IL-6 and TNF-α in liver were significantly increased (P<0.01, P<0.001). Conclusion: Periodontitis can increase the concentration of plasma TMAO in high-fat diet mice, which may be achieved by regulating gut microbiota related to TMAO metabolism.

Key words: periodontitis, trimethylamine N-oxide, gut microbiota

解伟格, 董静雯, 黄玲燕, 康文, 王璐, 吴娟, 谢思静. 牙周炎对高脂饮食小鼠血浆氧化三甲胺的影响[J]. 口腔医学研究, 2023, 39(1): 74-80.

XIE Weige, DONG Jingwen, HUANG Lingyan, KANG Wen, WANG Lu, WU Juan, XIE Sijing. Effects of Periodontitis on Plasma Trimethylamine N-Oxide in Mice Fed with High-fat Diet[J]. Journal of Oral Science Research, 2023, 39(1): 74-80.

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