口腔医学研究

口腔医学研究 ›› 2024, Vol. 40 ›› Issue (6): 512-518.DOI: 10.13701/j.cnki.kqyxyj.2024.06.008

• 骨生物学研究 • 上一篇    下一篇

静磁场对纯钛表面成骨细胞和巨噬细胞的影响

蒋沐岐1, 孙宇轩2, 孙亚生1, 聂志祥1, 张贞1*, 胡丽1*   

  1. 1.华中科技大学同济医学院附属协和医院口腔医学中心 湖北 武汉 430022;
    2.华中科技大学国家脉冲强磁场科学中心 湖北 武汉 430074
  • 收稿日期:2023-11-20 出版日期:2024-06-28 发布日期:2024-06-19
  • 通讯作者: * 张贞,E-mail: zhangzhentitanium@163.com胡丽,E-mail: huli_0316@163.com
  • 作者简介:蒋沐岐(1998~ ),女,武汉人,硕士在读,主要从事口腔材料与骨再生研究。
  • 基金资助:
    武汉强磁场学科交叉基金(编号:WHMFC202208)；华中科技大学研究生创新基金(编号:YCJJ202201052)

Effect of Static Magnetic Field on Osteoblasts and Macrophages on Pure Titanium.

JIANG Muqi1, SUN Yuxuan2, SUN Yasheng1, NIE Zhixiang1, ZHANG Zhen1*, HU Li1*   

  1. 1. Department of Stomatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China;
    2. Wuhan National High Magnetic Field Center, Huazhong University of Science and Technology, Wuhan 430074, China
  • Received:2023-11-20 Online:2024-06-28 Published:2024-06-19

PDF (PC)

18

摘要： 目的:本研究旨在探讨静磁场对纯钛表面成骨细胞及巨噬细胞的影响及机制。方法:在0 mT和100 mT静磁场作用下,细胞分别被接种到孔板表面和大颗粒喷砂酸蚀处理钛片表面。CCK-8法检测静磁场对成骨细胞及巨噬细胞活力的影响;细胞骨架荧光染色检测静磁场下成骨细胞及巨噬细胞的形态改变;免疫荧光染色检测静磁场下成骨细胞的骨特异性转录因子2(runt-related transcription factor 2,Runx2)蛋白表达;实时荧光定量聚合酶链反应(real-time fluorescence quantitative PCR,RT-qPCR)检测成骨细胞中成骨相关基因及巨噬细胞中免疫相关基因表达;流式细胞术检测巨噬细胞极化相关蛋白CD86、CD206表达水平。结果:100 mT静磁场可以有效促进钛片表面成骨细胞增殖,促进成骨相关基因碱性磷酸酶(alkaline phosphatase,ALP)、Ⅰ型胶原蛋白(collagen type Ⅰ, COL-1)、Runx2、骨保护蛋白(osteoprotegerin,OPG)表达,增强成骨相关蛋白Runx2合成;100 mT静磁场会促进巨噬细胞增殖,且能促进巨噬细胞第3天时向M1型极化,在第7天时会抑制炎症相关基因肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白细胞介素-1β(interleukin-1β,IL-1β)表达。结论:静磁场具有促进大颗粒喷砂酸蚀处理钛片表面成骨细胞增殖、矿化能力,以及促进钛表面巨噬细胞从前期促炎作用向后期抗炎作用的转变能力。

关键词: 静磁场, 钛, 成骨, 免疫

Abstract: Objective: To explore the effect and mechanism of static magnetic field on osteoblasts and macrophages on pure titanium surface. Methods: Under the static magnetic fields of 0 mT and 100 mT, cells were cultured on the surface of tissue culture well plates and sandblasting and acid etching (SLA-treated) titanium sheets, respectively. CCK-8 method was used to detect the effect of static magnetic field on the viability of osteoblasts and macrophages. Cytoskeleton fluorescence staining was used to detect the morphological changes of osteoblasts and macrophages under static magnetic field. Immunofluorescence staining was used to detect the expression of runt-related transcription factor 2 (Runx2), protein in osteoblasts under static magnetic field. The expression of osteogenesis-related genes in osteoblasts and immune-related genes in macrophages was detected by quantitative real-time fluorescence quantitative PCR (RT-qPCR). The expression levels of polarization-related proteins CD86 and CD206 in macrophages were detected by flow cytometry. Results: The static magnetic field of 100 mT could effectively promote the proliferation of osteoblasts on the titanium sheets, promote the expression of osteogenesis-related genes alkaline phosphatase (ALP), collagen type Ⅰ (COL-1), Runx2, and osteoprotegerin (OPG), and enhance the synthesis of osteogenesis-related protein Runx2. The static magnetic field of 100mT could promote the proliferation of macrophages, promote the polarization of macrophages to M1 type on the 3rd day, and inhibit the expression of inflammation-related genes tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) on the 7th day. Conclusion: Static magnetic field has the ability to promote the proliferation and mineralization of osteoblasts on the SLA-treated titanium sheets, and to promote the transformation of macrophages from pro-inflammatory to anti-inflammatory on the titanium sheets.

Key words: static magnetic field, titanium, osteogenesis, immune