Lugol's iodine增强Micro-CT成像评估口腔鳞状细胞癌切缘状态的研究

doi:10.13701/j.cnki.kqyxyj.2024.09.008

口腔医学研究 ›› 2024, Vol. 40 ›› Issue (9): 797-802.DOI: 10.13701/j.cnki.kqyxyj.2024.09.008

Lugol's iodine增强Micro-CT成像评估口腔鳞状细胞癌切缘状态的研究

郁佳鑫¹, 刘鲲宇¹, 章茜¹, 蒲玉梅², 胡勤刚¹, 夏成万^1*, 王育新^1*

1.南京大学医学院附属口腔医院,南京市口腔医院口腔颌面创伤正颌整形外科, 南京大学口腔医学研究所江苏南京 210008;
2.南京大学医学院附属口腔医院,南京市口腔医院口腔颌面头颈肿瘤外科, 南京大学口腔医学研究所江苏南京 210008

收稿日期:2024-03-25 出版日期:2024-09-28 发布日期:2024-09-25
通讯作者: ^*夏成万,E-mail:2665927917@qq.com,王育新,E-mail:wangyuxin0212@126.com
作者简介:郁佳鑫(1998~),女,江苏人,硕士在读,住院医师,研究方向:口腔颌面外科学。
基金资助:
江苏省重点研发计划项目(社会发展)(编号:BE2021609)南京市卫生科技发展项目(编号:YKK22177)

Study on Lugol's Iodine Enhanced Micro-CT Imaging for Evaluating Margin Status of Oral Squamous Cell Carcinoma

YU Jiaxin¹, LIU Kunyu¹, ZHANG Qian¹, PU Yumei², HU Qingang¹, XIA Chengwan^1*, WANG Yuxin^1*

1. Department of Oral and Maxillofacial Trauma Orthognathic Plastic Surgery, Nanjing Stomatological Hospital, Affiliated Hospital of Medical School, Research Institute of Stomatology, Nanjing University, Nanjing 210008, China;
2. Department of Oral and Maxillofacial Head and Neck Oncology Surgery, Nanjing Stomatological Hospital, Affiliated Hospital of Medical School, Research Institute of Stomatology, Nanjing University, Nanjing 210008, China

Received:2024-03-25 Online:2024-09-28 Published:2024-09-25

摘要/Abstract

摘要： 目的:本研究拟使用Lugol's iodine增强Micro-CT成像三维评估口腔鳞状细胞癌(oral squamous cell carcinoma,OSCC)切缘状态。方法:本研究共收集68例人OSCC组织样本,所有样本经3% Lugol's iodine染色12 h后行Micro-CT扫描(层厚为50 μm)。随后将经Lugol's iodine染色后的样品行常规苏木精-伊红(hematoxylin-eosin,HE)染色切片(厚度为4 μm),制作数字化病理切片。分别测量影像图像和病理图像的黏膜切缘(mucosal margin,MM)和深部切缘(deep margin,DM)。结果:Lugol's iodine增强Micro-CT成像可以在三维空间中清晰区分肿瘤边界, 且Micro-CT成像相较于二维病理切片,可以更准确地测量MM和DM,发现阳性切缘及临界切缘。结论:Lugol's iodine增强Micro-CT成像可以全面评估OSCC切缘,提供更全面、更清晰的肿瘤边缘信息,指导临床治疗方案选择和预后评估。

关键词: 口腔鳞状细胞癌, 显微计算机断层成像, 卢戈氏碘液, 三维

Abstract: Objective: To assess the incisal margin status of oral squamous cell carcinoma (OSCC) in three dimensions using Lugol's iodine enhanced Micro-CT imaging. Methods: A total of 68 human OSCC in vitro samples were collected for this study. All samples were stained with 3% solution of Lugol's iodine for 12 hours and underwent Micro-CT scanning with a layer thickness of 50 μm. Subsequently, the Lugol's iodine stained samples were subjected to routine hematoxylin-eosin (HE) staining sections (4 μm) to create digital pathological sections. The mucosal margin (MM) and deep margin (DM) were measured separately on both the imaging and pathological images. Results: Lugol's iodine enhanced Micro-CT imaging can effectively differentiate tumor boundaries in three-dimensional space, providing more accurate measurements of MM and DM compared to two-dimensional pathological sections, thus enabling identification of positive incisal margins and critical incisal margins. Conclusion: Lugol's iodine enhanced Micro-CT imaging allows comprehensive evaluation of OSCC incisal margins in three dimensions, offering more comprehensive and clear information regarding tumor margins which can guide clinical treatment plan selection and prognosis assessment.

Key words: oral squamous cell carcinoma, Micro-CT, Lugol's iodine, 3D

郁佳鑫, 刘鲲宇, 章茜, 蒲玉梅, 胡勤刚, 夏成万, 王育新. Lugol's iodine增强Micro-CT成像评估口腔鳞状细胞癌切缘状态的研究[J]. 口腔医学研究, 2024, 40(9): 797-802.

YU Jiaxin, LIU Kunyu, ZHANG Qian, PU Yumei, HU Qingang, XIA Chengwan, WANG Yuxin. Study on Lugol's Iodine Enhanced Micro-CT Imaging for Evaluating Margin Status of Oral Squamous Cell Carcinoma[J]. Journal of Oral Science Research, 2024, 40(9): 797-802.

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Lugol's iodine增强Micro-CT成像评估口腔鳞状细胞癌切缘状态的研究

Study on Lugol's Iodine Enhanced Micro-CT Imaging for Evaluating Margin Status of Oral Squamous Cell Carcinoma

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