炎性标志物在口腔黏膜病组织上皮-间质表达特征及其临床意义

doi:10.13701/j.cnki.kqyxyj.2026.01.008

口腔医学研究 ›› 2026, Vol. 42 ›› Issue (1): 41-47.DOI: 10.13701/j.cnki.kqyxyj.2026.01.008

炎性标志物在口腔黏膜病组织上皮-间质表达特征及其临床意义

陈奕霏¹, 牟镜天¹, 韩琪², 周红梅¹, 吴芳龙^1*

1.口腔疾病防治全国重点实验室国家口腔医学中心口腔疾病国家临床医学研究中心口腔医学⁺前沿医学中心四川大学华西口腔医院口腔黏膜病科四川成都 610041;
2.四川大学华西口腔医院口腔病理科四川成都 610041

收稿日期:2025-04-08 出版日期:2026-01-28 发布日期:2026-01-23
通讯作者: *吴芳龙,E-mail:wufanglong@scu.edu.cn
作者简介:陈奕霏(1999~ ),男,重庆人,硕士在读,研究方向:口腔黏膜病的发病机制及其防治。
基金资助:
国家自然科学基金(编号:82370963,82071124)

Expression Characteristics and Clinical Significance of Inflammatory Markers in Epithelium and Stroma of Oral Mucosal Diseases

CHEN Yifei¹, MOU Jingtian¹, HAN Qi², ZHOU Hongmei¹, WU Fanglong^1*

1. State Key Laboratory of Oral Diseases & National Center for Stomatology & National Clinical Research Center for Oral Diseases & Frontier Innovation Center for Dental Medicine Plus, Department of Oral Medicine, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China;
2. Department of Oral Pathology, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China

Received:2025-04-08 Online:2026-01-28 Published:2026-01-23

摘要/Abstract

摘要： 目的: 上皮-间质共同维持的免疫稳态是口腔黏膜健康的重要生物学基础。本研究通过检测炎性标志物在口腔黏膜病患者上皮和间质的表达模式,以探讨其临床意义,为不同炎症类型口腔黏膜病的临床管理决策提供依据。方法: 纳入2020年12月~2024年12月就诊的口腔黏膜病患者60例,其中,口腔黏膜大疱类疾病(oral bullous diseases,OBDs)、口腔扁平苔藓(oral lichen planus,OLP)和口腔白斑病(oral leukoplakia,OLK)各20例。通过免疫组织化学染色法检测口腔黏膜组织和细胞中血管紧张素转换酶2(angiotensin converting enzyme 2,ACE2)、跨膜蛋白酶丝氨酸2(transmembrane protease serine 2,TMPRSS2)、白细胞介素-1β(interleukin-1β,IL-1β)和补体C3(complement 3,C3)的表达情况,并进行上皮-间质表达特征及其临床意义分析。结果: 针对OBDs、OLP和OLK组织的免疫组织化学检测结果显示,ACE2主要在口腔黏膜组织的上皮棘层及基底层的细胞膜表达,而在间质细胞中表达较弱(P<0.01);TMPRSS2广泛表达于口腔黏膜上皮全层,在间质细胞中表达较弱(P<0.01) ;IL-1β和C3在OLK上皮和间质组织中的表达均显著高于OLP(P<0.01)。结论: ACE2和TMPRSS2主要参与维持口腔黏膜上皮屏障的基本功能,在OBDs、OLP和OLK的炎症病理变化过程中没有特异性;与OBDs和OLP相比,IL-1β和C3更适合用于OLK组织上皮和间质的炎症状态评估。

关键词: 口腔黏膜病, 炎性标志物, 免疫失衡, 上皮-间质, 临床意义

Abstract: Objective: To detect the expression pattern of inflammatory markers in the epithelium and stroma of the patients with oral mucosal diseases. Methods: Sixty patients, with 20 cases of oral bullous diseases (OBDs), 20 cases of oral lichen planus (OLP), and 20 cases of oral leukoplakia (OLK), from December 2020 to December 2024, were enrolled in present study. Immunohistochemical (IHC) staining was performed to assess the expressions of angiotensin-converting enzyme 2 (ACE2), transmembrane protease serine 2 (TMPRSS2), interleukin-1β (IL-1β), and complement C3 (Complement 3, C3) in oral mucosal tissues and cells. The epithelial-stromals expression characteristics and their clinical significance were analyzed. Results: IHC for OBDs, OLP, and OLK tissues results revealed that ACE2 expressed mainly in the cellular membranes of the epithelial stratum spinosum and basale, while weakly in the stromal cells (P<0.01). TMPRSS2 expressed widely in the whole epithelial layers, particularly in glandular ductal epithelium, while weakly in the stromal cells (P<0.01). The expressions of IL-1β and C3 in the epithelium and stroma of OLK were significantly higher than those of OLP (P<0.01). Conclusion: ACE2 and TMPRSS2 primarily participate in maintaining the basic functions of the oral mucosal epithelial barrier and do not exhibit specificity in the inflammatory pathological changes of OBDs, OLP, and OLK. Compared to OBDs and OLP, IL-1β and C3 are more suitable for assessing the inflammatory status of the epithelium and stroma in OLK tissues.

Key words: oral mucosal diseases, inflammatory markers, immune imbalance, epithelium-stroma, clinical significance

陈奕霏, 牟镜天, 韩琪, 周红梅, 吴芳龙. 炎性标志物在口腔黏膜病组织上皮-间质表达特征及其临床意义[J]. 口腔医学研究, 2026, 42(1): 41-47.

CHEN Yifei, MOU Jingtian, HAN Qi, ZHOU Hongmei, WU Fanglong. Expression Characteristics and Clinical Significance of Inflammatory Markers in Epithelium and Stroma of Oral Mucosal Diseases[J]. Journal of Oral Science Research, 2026, 42(1): 41-47.

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炎性标志物在口腔黏膜病组织上皮-间质表达特征及其临床意义

Expression Characteristics and Clinical Significance of Inflammatory Markers in Epithelium and Stroma of Oral Mucosal Diseases

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