原位法合成纳米银对树脂粘结剂抗菌改性的应用研究

doi:10.13701/j.cnki.kqyxyj.2018.02.013

口腔医学研究 ›› 2018, Vol. 34 ›› Issue (2): 161-164.DOI: 10.13701/j.cnki.kqyxyj.2018.02.013

原位法合成纳米银对树脂粘结剂抗菌改性的应用研究

范玥, 孟翔峰^*

南京大学医学院附属口腔医院,南京市口腔医院修复科江苏南京 210008;

收稿日期:2017-11-15 出版日期:2018-02-28 发布日期:2018-02-26
通讯作者: 孟翔峰,电话:025-83620263
作者简介:范玥(1992~ ),女,福建人,医师,硕士,主要从事口腔修复学工作。
基金资助:
国家自然科学基金资助项目(编号:81470781)
江苏省自然科学基金项目(编号:BK20141082)

Application of in Situ Synthesized Silver Nanoparticles to Antibacterial Modification of Resin Adhesives

FAN Yue, MENG Xiang-feng^*

Nanjing Stomatological Hospital, Medical School of Nanjing University. Nanjing 210008, China.

Received:2017-11-15 Online:2018-02-28 Published:2018-02-26

摘要/Abstract

摘要： 目的:运用原位法合成纳米银对树脂粘结剂进行抗菌改性,并评价其抗菌性能。方法:将2-乙基己酸银添加到甲基丙烯酸叔丁基氨基乙酯中形成8wt%的银源溶液,再将其添加到Clearfil SE Bond的树脂粘结剂中,获得含2-乙基己酸银浓度为0wt%、0.10wt%、0.20wt%的树脂粘结剂。将树脂粘结剂制成直径为6 mm,厚度为1 mm的试件,再分别接受20 s,1 000 mW/cm²光强度照射完成固化。用透射电镜观察固化后试件中纳米银的生成及分散情况,粒径大小及分布;将试件分别浸泡在无菌人工唾液中1 d和1周,采用直接接触法检测固化后试件表面对变形链球菌的抗菌活性,并采用荧光共聚焦显微镜观察其对细胞胞膜的影响。结果:透射电镜观察到0.10wt%和0.20wt%组试件中有纳米颗粒生成,分散均匀,平均粒径分别为(3.12±0.82) nm,(3.64±0.65) nm;0wt%、0.10wt%和0.20wt%组试件浸泡1 d后均有显著的抗菌活性,且抗菌率无显著差异;浸泡1周后,0wt% 组抗菌率降低,0.10wt%和0.20wt%组抗菌率提高,并显著高于0wt%组的。结论:原位法合成纳米银在树脂粘结剂的抗菌改性中的应用是可行的。

关键词: 纳米银, 树脂粘结剂, 抗菌性, 变形链球菌

Abstract: Objective: To endow the resin bonding agents with antibacterial characteristic by applying the in situ synthesized silver nanoparticles, and to evaluate the antibacterial properties of resin bonding agents. Methods: The sliver 2-ethylhexanoate was dissolved into 2-(tert-butylamino) ethyl methacrylate to form a silver amine solution with a mass fraction of 8%. Then the silver amine solution was added to the adhesive part of Clearfil SE Bond, obtained three resin adhesives having 0wt%, 0.10wt%, and 0.20wt% of 2-ethylhexnoate respectively. All groups were irradiated by 1 000 mW/cm² for 20s to form specimens with diameter of 6mm and thickness of 1 mm. The size and distribution of silver nanoparticles were observed and measured by transmission electron microscope. Specimens were separately immersed in the sterile artificial saliva for 1 day or 1 week. After the saliva immersion, the resistance to streptococcus mutans of each group was calculated by colony counting method, and the antibacterial effect on the bacterial membrane was observed using a live/dead bacterial staining method under laser confocal microscopy. Results: The mean particle sizes of the group of 0.10wt% and 0.20wt% under transmission electron microscopy were respectively 3.12±0.82 nm and 3.64±0.65 nm, and the dispersion of the silver nanoparticles were evenly. The antimicrobial activity was significant after immersing in saliva for 1 day. After 1 week of immersion, the antibacterial activity of the 0wt% group was decreased, and the antimicrobial activity of 0.10wt% and 0.20wt% were increased, and significantly higher than that of 0wt% group (P<0.05). Conclusion: The application of in situ synthesized silver nanoparticles is feasible in the antibacterial modification of resin bonding agent.

Key words: Silver nanoparticles, Self-etching adhesive, Antibacterial activity, Streptococcus mutans

中图分类号:

R783.1

范玥, 孟翔峰. 原位法合成纳米银对树脂粘结剂抗菌改性的应用研究[J]. 口腔医学研究, 2018, 34(2): 161-164.

FAN Yue, MENG Xiang-feng. Application of in Situ Synthesized Silver Nanoparticles to Antibacterial Modification of Resin Adhesives[J]. Journal of Oral Science Research, 2018, 34(2): 161-164.

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原位法合成纳米银对树脂粘结剂抗菌改性的应用研究

Application of in Situ Synthesized Silver Nanoparticles to Antibacterial Modification of Resin Adhesives

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