口腔医学研究

口腔医学研究 ›› 2019, Vol. 35 ›› Issue (11): 1066-1069.DOI: 10.13701/j.cnki.kqyxyj.2019.11.014

• 口腔材料学研究 • 上一篇    下一篇

原位合成纳米银树脂粘结剂的长期抗菌及粘结性能研究

范玥, 任灵燕, 汤旭娜, 孟翔峰*   

  1. 南京大学医学院附属口腔医院,南京市口腔医院 江苏 南京 210008
  • 收稿日期:2019-02-02 出版日期:2019-11-28 发布日期:2019-11-21
  • 通讯作者: 孟翔峰,电话:025-83620263
  • 作者简介:范玥(1992~ ),女,福建人,医师,硕士,主要从事口腔修复学工作。
  • 基金资助:
    国家自然科学基金(编号:81470781) 南京市科技计划项目(编号:201715040) 南京市医学科技发展资金项目(编号:YKK16157)

Study on Long-term Antibacterial and Bonding Properties of Silver Nanoparticles In-situ Synthesized in Resin Adhesive

FAN Yue, REN Lingyan, TANG Xuna, MENG Xiangfeng*   

  1. Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing 210008, China
  • Received:2019-02-02 Online:2019-11-28 Published:2019-11-21

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摘要： 目的: 评估原位法合成纳米银树脂粘结剂的聚合度、粘结强度及长期抗菌效果。方法: 将2-乙基己酸银添加到甲基丙烯酸叔丁基氨基乙酯中形成8wt%的银源溶液,再将其添加到Clearfil SE Bond(CSB)的树脂粘结剂中,获得0wt% Ag、0.10wt% Ag、0.20wt% Ag的3组树脂粘结剂,并接受1000 mW/cm2光强度照射20 s,制成直径为6 mm,厚度为1 mm的试件;将试件分别浸泡在无菌人工唾液中1周和12周,采用直接接触法检测表面对变形链球菌的抗菌活性,并用扫描电子显微镜观察表面细菌生物膜的形成;使用傅里叶红外光谱仪检测试件固化前后的C=C转化度,计算聚合度;使用微拉伸实验方法评估各组树脂粘结剂与牙本质间的粘结强度。结果: 3组树脂粘结剂的聚合度、微拉伸强度均无显著差异。与浸泡1周后相比,浸泡12周后的3组粘结剂抗菌率均显著降低,其中0wt% Ag组不具有抗菌效果,而0.10wt% Ag和0.20wt% Ag组的抗菌率分别为87.00%和93.48%。结论: 原位法合成纳米银能够为树脂粘结剂提供较好的长期抗菌性能,并对其聚合度和粘结性能无显著影响。

关键词: 纳米银, 树脂粘结剂, 抗菌性, 聚合度, 粘结强度

Abstract: Objective: To evaluate the degree of polymerization, the long-term antibacterial, and bonding properties of resin adhesive containing silver nanoparticles. Methods: Silver 2-ethylhexanoate was added to 2-(tert-butylamino) ethyl methacrylate to form a 8 wt% silver salt containing solution. Then the mixed solution was added to the adhesive part of Clearfil SE Bond (CSB) to obtain three resin adhesives containing 0wt%, 0.10wt%, and 0.20wt% Ag. All specimens (diameter of 6 mm, thickness of 1 mm) were irradiated by 1000 mW/cm2 for 20 s. Specimens were separately immersed in the sterile artificial saliva for 1 week and 12 weeks. The direct contact method was used to detect the antibacterial activity against Streptococcus mutans, and the formation of biofilms was observed by scanning electron microscope. The degree of conversion of specimens was measured by Fourier transform infrared spectrometer. And the micro-tensile bond strength of specimens was measured after soaking in artificial saliva for 1 d and 12 w. Results: There was no significant difference in the degree of conversion and micro-tensile bond strength above three resin adhesives (P>0.05). After immersion for 12 weeks, the 0 wt% Ag resin adhesive had no antibacterial ability, while the antibacterial rates of 0.10wt% Ag and 0.20wt% Ag adhesive were 87.00% and 93.48%, respectively. Conclusion: In-situ synthesis of silver nanoparticles could provide antibacterial properties for resin adhesives, and had no effect on the degree of conversion and bonding property of adhesives.

Key words: silver nanoparticles, resin adhesive, antibacterial activity, degree of conversion, microtensile strength