口腔医学研究

口腔医学研究 ›› 2020, Vol. 36 ›› Issue (6): 581-584.DOI: 10.13701/j.cnki.kqyxyj.2020.06.018

• 口腔材料学研究 • 上一篇    下一篇

两种抛光方式对两种充填材料表面形貌和细胞增殖能力的影响

尚佳鑫1, 苏瑞2, 马冲3, 李新月2*   

  1. 1.南开大学医学院 天津 300071;
    2.天津市口腔医院 天津 300041;
    3.泰康拜博口腔医院 天津 300000
  • 收稿日期:2019-10-08 出版日期:2020-07-03 发布日期:2020-07-06
  • 通讯作者: *李新月,E-mail:lisaxinyue1215@126.com
  • 作者简介:尚佳鑫(1994~ ),女,辽宁鞍山人,硕士在读,医师,研究方向:牙周病学。

Effects of Polishing Methods on Surface Morphology of and Cell Proliferation of Human Gingival Fibroblasts on Two Kinds of Filling Materials

SHANG Jiaxin1, SU Rui2, MA Chong3, LI Xinyue2*   

  1. 1. Medical College, NanKai University, Tianjin 300071, China;
    2. Tianjin Stomatological Hospital, Tianjin 300041, China;
    3. Taikangbaibo Stomatological Hospital, Tianjin 300000, China
  • Received:2019-10-08 Online:2020-07-03 Published:2020-07-06

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摘要： 目的: 观察两种抛光处理后的两种材料表面形态和细胞生物相容性。方法: 采用树脂改性玻璃离子GC Fuji Ⅱ LC、纳米复合树脂3M FiltekTM Z350 XT制备试件,均分别采用矽粒子和Super-Snap抛光系统进行抛光,扫描电镜观察材料和牙根片表面形貌。在试件上接种人牙龈成纤维细胞,第1、3、5、7天通过MTT法进行细胞增殖试验。结果: 5000倍电镜下,树脂较玻璃离子表面更光滑,树脂联合抛光系统或矽粒子较玻璃离子联合抛光系统或矽粒子表面更光滑。细胞试验中,第5天树脂联合Super-Snap较玻璃离子联合矽粒子和Super-Snap的A值均较高,树脂联合矽粒子组较玻璃离子联合矽粒子吸光度值高,差异均有统计学意义。结论: 3M FiltekTM Z350 XT树脂联合Super-Snap抛光系统可获得较光滑的表面,Super-Snap抛光系统较矽粒子抛光处理可获得更好的细胞相容性。

关键词: 复合树脂, 玻璃离子, 人牙龈成纤维细胞, 扫描电镜, 抛光, 细胞增殖

Abstract: Objective: To observe the surface morphology and biocompatibility of the two polished materials. Methods: The specimens were prepared by using resin-modified glass ionomer GC Fuji Ⅱ LC and nano-composite resin 3M FiltekTM Z350 XT. Silicon particles and super-snap polishing system were used for polishing. The surface morphology of the materials and the root slices were observed by SEM. Human gingival fibroblasts were inoculated on the specimens, and cell proliferation test was conducted by MTT method on the 1st, 3rd, 5th and 7th day. Results: Under 5000 times electron microscope, the surface of resin was smoother than that of glass ionomer, and the surface of resin combined polishing system or silicon particle was smoother than that of glass ion combined polishing system or silicon particle. In the cell proliferation test, the 5d resin combined with super-snap had higher absorbance values than both glassy ion combined with silicon particles and super-snap, and the resin combined with silicon particles had higher absorbance values than glassy ion combined with silicon particles (P<0.05). Conclusion: 3M FiltekTM Z350 XT resin combined with super-snap polishing system can obtain a smoother surface, and the super-snap polishing system can obtain better cell compatibility than the silicon particle polishing treatment.

Key words: composite resin, glass ionomer, human gingival fibroblasts, scanning electron microscope, polishing, cell proliferation