D/L-缬氨酸对牙龈卟啉单胞菌及其生物膜的作用

doi:10.13701/j.cnki.kqyxyj.2020.07.010

口腔医学研究 ›› 2020, Vol. 36 ›› Issue (7): 648-653.DOI: 10.13701/j.cnki.kqyxyj.2020.07.010

D/L-缬氨酸对牙龈卟啉单胞菌及其生物膜的作用

张慧彦^1,2, 李保胜¹, 张震阳^1,2, 韩春雨^1,2, 李尊泰^1,2, 齐晓爽^1,2, 付齐月^1,2, 孟维艳^1,2*

1.吉林大学口腔医院口腔种植中心吉林长春 130000;
2.吉林省牙发育及颌骨重塑与再生重点实验室吉林长春 130000

收稿日期:2019-10-21 出版日期:2020-07-28 发布日期:2020-07-24
通讯作者: 孟维艳,E-mail:mengsitong66@163.com
作者简介:张慧彦(1993~),男,吉林人,硕士在读,研究方向:口腔种植学、牙周病学。
基金资助:
国家自然科学基金(编号:81571007) ;吉林省科技发展自然科学基金(编号:20180101121JC)

Effects of D/L-valine on Porphyromonas Gingivalis and Porphyromonas Gingivalis Biofilm

ZHANG Huiyan^1,2, LI Baosheng¹, ZHANG Zhenyang^1,2, HAN Chunyu^1,2, LI Zuntai^1,2, QI Xiaoshuang^1,2, FU Qiyue^1,2, MENG Weiyan^1,2*

1. Department of Dental Implantology, School and Hospital of Stomatology, Changchun 130000, China;
2. Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling, Jilin University, Changchun 130000, China

Received:2019-10-21 Online:2020-07-28 Published:2020-07-24

摘要/Abstract

摘要： 目的: 研究D-缬氨酸与L-缬氨酸对牙龈卟啉单胞菌及其生物膜的作用。方法: 通过CCK-8实验获得无细胞毒性的D-缬氨酸浓度;通过生长曲线实验评估D-缬氨酸与L-缬氨酸对牙龈卟啉单胞菌细菌活性的作用;通过结晶紫染色实验、胞外多糖实验和扫描电镜评估D-缬氨酸与L-缬氨酸对牙龈卟啉单胞菌生物膜形成的抑制作用和对成熟牙龈卟啉单胞菌生物膜的分散作用。结果: D-缬氨酸在浓度为50 mmol/L和75 mmol/L时对成纤维细胞无细胞毒性;D-缬氨酸(75 mmol/L)能有效抑制牙龈卟啉单胞菌的细菌活性和生物膜的形成,并分散成熟的生物膜。D-缬氨酸(50 mmol/L)在72 h内抑制牙龈卟啉单胞菌的生物活性和生物膜的形成。L-缬氨酸(75 mmol/L和50 mmol/L)促进牙龈卟啉单胞菌的细菌活性和生物膜的形成。当D-缬氨酸和L-缬氨酸同时存在时,仅表现出D-缬氨酸的作用。结论: D-缬氨酸具有抑制牙龈卟啉单胞菌细菌活性和生物膜形成,并分散成熟生物膜的作用;L-缬氨酸能促进牙龈卟啉单胞菌的细菌活性和生物膜的形成,但其作用与D-缬氨酸不相拮抗。D-缬氨酸可作为治疗菌斑生物膜相关疾病的潜在生物制剂。

关键词: D-氨基酸, D-缬氨酸, 牙龈卟啉单胞菌, 生物膜

Abstract: Objective: To study the effects of D-valine and L-valine on porphyromonas gingivalis (P.gingivalis) and its biofilms. Methods: The concentration of D-valine without cytotoxicity was obtained by CCK-8 experiment. The effects of D-valine and L-valine on the bacterial activity of P.gingivalis were evaluated by growth curve experiment. The inhibition of D-valine and L-valine on the biofilm formation of P.gingivalis and the dispersion of mature P.gingivalis biofilm were evaluated by crystal violet staining experiment, extracellular polysaccharide experiment, and scanning electron microscope. Results: D-valine had no cytotoxicity to fibroblasts at 50 mmol/L and 75 mmol/L concentrations. D-valine (75 mmol/L) could effectively inhibit the bacterial activity and biofilm formation of P.gingivalis and destroyed the mature P.gingivalis biofilm. D-valine (50 mmol/L) inhibited the bioactivity and biofilm formation of P.gingivalis for 72 h. L-valine (75 mmol/L and 50 mmol/L) promoted the bacterial activity of P.gingivalis and the formation of biofilm. When D-valine and L-valine existed simultaneously, D-valine functions were shown only. Conclusion: D-valine can inhibit the bacterial activity and biofilm formation of P.gingivalis and destroy the mature biofilm. L-valine can promote the bacterial activity and biofilm formation of P.gingivalis, but it is not antagonistic to D-valine. D-valine can be used as a potential biologic agent to treat bacterial plaque related diseases.

Key words: D-amino acid, D-valine, porphyromonas gingivalis, biofilm

张慧彦, 李保胜, 张震阳, 韩春雨, 李尊泰, 齐晓爽, 付齐月, 孟维艳. D/L-缬氨酸对牙龈卟啉单胞菌及其生物膜的作用[J]. 口腔医学研究, 2020, 36(7): 648-653.

ZHANG Huiyan, LI Baosheng, ZHANG Zhenyang, HAN Chunyu, LI Zuntai, QI Xiaoshuang, FU Qiyue, MENG Weiyan. Effects of D/L-valine on Porphyromonas Gingivalis and Porphyromonas Gingivalis Biofilm[J]. Journal of Oral Science Research, 2020, 36(7): 648-653.

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D/L-缬氨酸对牙龈卟啉单胞菌及其生物膜的作用

Effects of D/L-valine on Porphyromonas Gingivalis and Porphyromonas Gingivalis Biofilm

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