口腔医学研究

口腔医学研究 ›› 2021, Vol. 37 ›› Issue (4): 310-313.DOI: 10.13701/j.cnki.kqyxyj.2021.04.008

• 牙周病学研究 • 上一篇    下一篇

雷尼酸锶对实验性牙周炎大鼠牙槽骨改建的影响

梁向阳, 李春年*   

  1. 河北医科大学口腔医学院·河北省口腔医学重点实验室,河北省口腔疾病临床医学研究中心 河北 石家庄 050017
  • 收稿日期:2020-10-26 发布日期:2021-04-15
  • 通讯作者: *李春年,E-mail:lchunnian@163.com
  • 作者简介:梁向阳(1990~ ),女,河北邢台人,硕士,主治医师,主要从事口腔内科学研究工作。
  • 基金资助:
    河北省医学科学研究重点课题(编号:20160213)

Effect of Strontium Ranelate on Alveolar Bone Remodeling in Experimental Periodontitis Rats

LIANG Xiangyang, LI Chunnian*   

  1. School and Hospital of Stomatology, Hebei Medical University & Hebei Key Laboratory of Stomatology, The Oral Disease Clinical Medicina Research Institution of Hebei, Shijiazhuang 050017, China
  • Received:2020-10-26 Published:2021-04-15

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摘要： 目的: 通过观察雷尼酸锶对大鼠实验性牙周炎治疗后牙槽骨组织中酸性磷酸酶及碱性磷酸酶的变化,评估雷尼酸锶对实验性牙周炎的治疗作用,为应用雷尼酸锶治疗牙周炎提供理论依据和实验参考。方法: 选取50只3月龄雄性SD大鼠,随机分为5组:正常组(A组)、牙周炎组(B组)、牙周炎局部治疗组(C组)、牙周炎雷尼酸锶治疗组(D组)、牙周炎局部治疗+雷尼酸锶治疗组(E组),每组10只。所有的实验性牙周炎大鼠通过正畸钢丝结扎双侧上颌第一磨牙建立牙周炎模型,C、E组通过去除结扎丝、局部上药建立实验性牙周炎局部治疗模型,D、E组通过雷尼酸锶灌胃建立雷尼酸锶治疗模型;大鼠钢丝结扎4周后随机抽取大鼠鉴定牙周炎模型成功,钢丝结扎8周后建立牙周炎局部治疗模型,雷尼酸锶治疗组自钢丝结扎8周后开始灌胃治疗。牙周治疗、药物治疗8周后处死大鼠,制作牙周组织切片,观察牙周组织变化情况;计数牙周单位面积成骨细胞和破骨细胞数目;进行抗酒石酸酸性磷酸酶染色(TRAP)及碱性磷酸酶染色,评价牙槽骨的变化。结果: 在牙周局部治疗、雷尼酸锶治疗后,牙周炎症减轻,牙周袋变浅,单位面积破骨细胞显著减少,成骨细胞数量显著增多,在牙周局部治疗和雷尼酸锶联合治疗组尤为明显。结论: 雷尼酸锶作为骨质疏松治疗药物,也能对实验性牙周炎产生确定的疗效,可以为牙周炎的综合治疗提供新的治疗思路。

关键词: 牙周炎, 雷尼酸锶, 酸性磷酸酶, 碱性磷酸酶, 成骨细胞, 破骨细胞

Abstract: Objective: To evaluate the effects of strontium ranelate on experimental periodontitis rats. Methods: Fifty 3-month-old male SD rats were randomly divided into five groups (10 rats/group): normal group (group A), periodontitis group (group B), periodontitis with locally treating group (group C), periodontitis with strontium ranelate treating group (group D), and periodontitis with locally treating + strontium ranelate treating group (group E). Periodontitis model was established in group B, C, D, and E. Group C was treated by local drug application. Group D was treated by intragastric administration with strontium ranelate. Group E was treated by local drug application and intragastric administration. The rats were sacrificed after 8 weeks of periodontal treatment and drug treatment. The number of osteoblasts and osteoclasts in per periodontal unit area was counted. Tartrate resistant acid phosphatase (TRAP) and alkaline phosphatase (ALP) staining were performed to evaluate the alveolar bone metabolism. Results: After local treatment and strontium ranelate treatment, the inflammation of periodontal tissue was reduced, and periodontal pockets became shallower, the osteoclasts stained with TRAP in per unit area decreased significantly, and the osteoblasts stained with ALP increased significantly. Conclusion: Strontium ranelate, as a therapeutic agent for osteoporosis, can also have a certain effect on the treatment of experimental periodontitis, and provide new idea for the comprehensive treatment of periodontitis.

Key words: periodontitis, strontium ranelate, acid phosphatase, alkaline phosphatase, osteoblast, osteoclast