积雪草酸抑制变异链球菌生物膜形成及致龋能力的研究

doi:10.13701/j.cnki.kqyxyj.2021.05.006

口腔医学研究 ›› 2021, Vol. 37 ›› Issue (5): 401-406.DOI: 10.13701/j.cnki.kqyxyj.2021.05.006

积雪草酸抑制变异链球菌生物膜形成及致龋能力的研究

税钰森¹, 杨燃^2*

1.口腔疾病研究国家重点实验室国家口腔疾病临床医学研究中心华西口腔医学院(四川大学) 四川成都 610041;
2.口腔疾病研究国家重点实验室国家口腔疾病临床医学研究中心华西口腔医院儿童口腔科(四川大学) 四川成都 610041

收稿日期:2020-11-30 发布日期:2021-05-17
通讯作者: * 杨燃,E-mail:yangran@scu.edu.cn
作者简介:税钰森(1998~ ),男,四川人,学士,研究方向:龋病生物学。
基金资助:
国家自然科学基金(编号:81800989)

Anti-cariogenic and Anti-biofilm Effect of Asiatic Acid on Streptococcus Mutans

SHUI Yusen¹, YANG Ran^2*

1. State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University. Chengdu 610041, China;
2. State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Dept. of Pediatric Dentistry, West China Hospital of Stomatology, Sichuan University. Chengdu 610041, China

Received:2020-11-30 Published:2021-05-17

摘要/Abstract

摘要： 目的:研究积雪草酸对变异链球菌(Streptococcus mutans, S. mutans)生物膜的抑制作用及对其产酸和产胞外多糖(extracellular polysaccharides,EPS)的影响。方法:通过药敏试验测定积雪草酸对 S. mutans浮游菌的最低抑菌浓度( Minimum inhibitory concentration,MIC);结晶紫试验和MTT试验分别探究积雪草酸对S. mutans生物膜的总量和代谢活性的影响;采用蒽酮-硫酸法,场发射扫描电镜(Field emission scanning electron microscopy,FESEM)和激光共聚焦显微镜(Confocal laser scanning microscopy,CLSM)测定积雪草酸对S. mutans生物膜EPS的产生和结构形貌的影响;pH计测定积雪草酸对 S. mutans产酸的影响;CCK-8法检测积雪草酸的细胞毒性。结果:积雪草酸对S. mutans的最低抑菌浓度为20 μg/mL,在亚抑菌浓度(10 μg/mL和5 μg/mL)下均能有效抑制 S. mutans生物膜形成以及产酸和产EPS。同时,积雪草酸无明显的细胞毒性。结论:积雪草酸在一定浓度下能有效抑制S. mutans生物膜形成,并能降低其产酸和产EPS等致龋毒力。

关键词: 积雪草酸, 变异链球菌, 生物膜

Abstract: Objective: To analyze the inhibitory effect of asiatic acid on Streptococcus mutans (S.mutans) biofim formation and its acid and extracellular polysaccharides (EPS) production. Methods: The planktonic bacterial susceptibility assay was used to determine the minimum inhibitory concentration (MIC) of asiatic acid on S.mutans. Crystal violet assay and MTT assay were performed to determine the effect of asiatic acid on the biomass and metabolic activity of S.mutans biofilm. Anthrone-sulfuric acid method, field emission scanning electron microscopy (FESEM), and confocal laser scanning microscopy (CLSM) were performed to observe the biofilm morphology and EPS synthesis after treatment by asiatic acid. The effect of asiatic acid on the acid production of S. mutans was performed by pH drop assay. CCK-8 kit assay was conducted to assess the cytotoxicity of asiatic acid. Results: The MIC of asiatic acid on S. mutans was 20 μg/mL. The sub-MICs (10 μg/mL and 5 μg/mL) were all effective on the reduction of the biofilm formation, acid production and EPS synthesis of S. mutans. No significant cytotoxicity of asiatic acid was found. Conclusion: Asiatic acid is effective on the inhibition of S.mutans biofilm formation, its acid production, and EPS synthesis

Key words: asiatic acid, Streptococcus mutans, biofilm

税钰森, 杨燃. 积雪草酸抑制变异链球菌生物膜形成及致龋能力的研究[J]. 口腔医学研究, 2021, 37(5): 401-406.

SHUI Yusen, YANG Ran. Anti-cariogenic and Anti-biofilm Effect of Asiatic Acid on Streptococcus Mutans[J]. Journal of Oral Science Research, 2021, 37(5): 401-406.

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积雪草酸抑制变异链球菌生物膜形成及致龋能力的研究

Anti-cariogenic and Anti-biofilm Effect of Asiatic Acid on Streptococcus Mutans

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