槲皮素脂质体对糖尿病牙周炎大鼠牙周组织的作用及血清AGEs的影响

doi:10.13701/j.cnki.kqyxyj.2021.07.011

口腔医学研究 ›› 2021, Vol. 37 ›› Issue (7): 628-631.DOI: 10.13701/j.cnki.kqyxyj.2021.07.011

槲皮素脂质体对糖尿病牙周炎大鼠牙周组织的作用及血清AGEs的影响

郭晓雨, 李淑娟, 梁向阳, 齐雅丽, 李雅, 杨冬茹^*

河北医科大学口腔医学院·口腔医院牙周一科,河北省口腔医学重点实验室,河北省口腔疾病临床医学研究中心河北石家庄 050017

收稿日期:2020-12-28 出版日期:2021-07-28 发布日期:2021-07-13
通讯作者: * 杨冬茹,E-mail:yangdr@hebmu.edu.cn
作者简介:郭晓雨(1993~ ),女,河北邯郸人,硕士,医师,主要从事牙周病学研究。
基金资助:
河北省医学科学研究课题(编号:20190100);河北省卫健委政府资助省级临床医学优秀人才培养和基础课题研究项目(编号:2019061441-2);河北省医学科学研究重点课题计划(编号:20180607)

Effect of Quercetin on Periodontal Tissues and Serum AGEs Levels in Rats with Diabetic Periodontitis

GUO Xiaoyu, LI Shujuan, LIANG Xiangyang, QI Yali, LI Ya, YANG Dongru^*

Department of NO.1 Periodontics, School and Hospital of Stomatology, Hebei Medical University & Hebei Key Laboratory of Stomatology, Hebei Provincial Oral Disease Clinical Medicine Research Center, Shijiazhuang 050017, China

Received:2020-12-28 Online:2021-07-28 Published:2021-07-13

摘要/Abstract

摘要： 目的:通过对Ⅱ型糖尿病牙周炎大鼠进行槲皮素干预,观察大鼠牙周组织的组织学变化、牙槽骨吸收量、血糖值变化以及血清晚期糖基化终末产物(advanced glycation end products,AGEs)的变化,探讨槲皮素对糖尿病牙周炎大鼠牙周组织的保护作用。方法:40只雄性SD大鼠,随机分成4组:空白对照组(N组)、牙周炎对照组(P组)、糖尿病牙周炎非治疗组(DP组)和糖尿病牙周炎槲皮素治疗组(DP+Q组)。N组正常喂养,P组建立牙周炎模型,DP组和DP+Q组建立糖尿病牙周炎模型,每组10只。建模成功后DP+Q组灌喂槲皮素脂质体,DP组灌喂空白脂质体,给药6周后测量各组血糖值,ELISA法测量血清AGEs含量,取上颌骨做牙周组织切片观察牙周组织病理变化,测量牙槽骨吸收量。结果:P组牙槽骨吸收量、牙周组织炎症水平高于N组(P＜0.05);DP组血糖值、血清AGEs含量、牙周组织炎症程度、牙槽骨吸收量均高于N组和P组(P＜0.05);DP+Q组大鼠血糖值、血清AGEs含量、牙周组织炎症水平、牙槽骨吸收量均低于DP组(P＜0.05)。结论:槲皮素对糖尿病牙周炎大鼠的牙周组织有保护效果,其作用机制与降低血糖值与血清AGEs含量有关。

关键词: 糖尿病, 牙周炎, 槲皮素, 晚期糖基化终末产物, 大鼠

Abstract: Objective: To observe the periodontal tissue morphology, alveolar bone resorption, blood glucose, and the differences in the content of serum advanced glycation end products (AGEs) through quercetin treatment in type Ⅱ diabetic periodontitis rats, and explore the protective effect of quercetin on the periodontal tissue of diabetic periodontitis rats. Methods: Forty male SD rats were randomly divided into 4 groups: blank control group (N group), periodontitis control group (P group), diabetic periodontitis non-treatment group (DP group), and diabetic periodontitis quercetin treatment group (DP+ Q group). The N group was fed normally, the P group was established a periodontitis model, and the DP group and DP+Q group were established diabetic periodontitis models, with 10 rats in each group. After successful modeling, the DP+Q group was gavaged with quercetin liposomes, and the DP group was gavaged with blank liposomes. After 6 weeks, blood glucose was measured, serum AGEs content was measured by ELISA method, periodontal tissue section of maxilla was taken to observe the pathological changes of periodontal tissue, and the amount of alveolar bone absorption was measured. Results: The amount of alveolar bone resorption and periodontal inflammation in P group were higher than those in N group (P<0.05). The blood glucose level, serum AGEs content, periodontal tissue inflammation, and alveolar bone absorption in the DP group were higher than those in the N group and P group (P<0.05). Compared with the DP group, the rats in the DP+Q group had lower blood glucose levels, lower serum AGEs content, lower alveolar bone absorption, and significantly lighter periodontal tissue inflammation (P<0.05). Conclusion: Quercetin has a protective effect on the periodontal tissues of diabetic periodontitis rats, and the mechanism is related to the lowering of blood glucose and the content of serum AGEs.

Key words: diabetes, periodontitis, quercetin, advanced glycation end products, rats

郭晓雨, 李淑娟, 梁向阳, 齐雅丽, 李雅, 杨冬茹. 槲皮素脂质体对糖尿病牙周炎大鼠牙周组织的作用及血清AGEs的影响[J]. 口腔医学研究, 2021, 37(7): 628-631.

GUO Xiaoyu, LI Shujuan, LIANG Xiangyang, QI Yali, LI Ya, YANG Dongru. Effect of Quercetin on Periodontal Tissues and Serum AGEs Levels in Rats with Diabetic Periodontitis[J]. Journal of Oral Science Research, 2021, 37(7): 628-631.

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槲皮素脂质体对糖尿病牙周炎大鼠牙周组织的作用及血清AGEs的影响

Effect of Quercetin on Periodontal Tissues and Serum AGEs Levels in Rats with Diabetic Periodontitis

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