EGCG和黄芩苷协同mTOR依赖性抑制小鼠牙周炎巨噬细胞M1向极化

doi:10.13701/j.cnki.kqyxyj.2021.07.010

口腔医学研究 ›› 2021, Vol. 37 ›› Issue (7): 622-627.DOI: 10.13701/j.cnki.kqyxyj.2021.07.010

EGCG和黄芩苷协同mTOR依赖性抑制小鼠牙周炎巨噬细胞M1向极化

周毅¹, 陈珍¹, 刘杨若萱¹, 胡礼群¹, 袁敏彦¹, 闫璐¹, 黄声富^2*, 李宇红^3*

1.湖北省口腔基础医学重点实验室-省部共建国家重点实验室培训基地和口腔生物医学教育部重点实验室湖北武汉 430079;
2.武汉大学口腔医院正畸科湖北武汉 430079;
3.武汉大学口腔医院牙体牙髓科湖北武汉 430079

收稿日期:2020-11-16 出版日期:2021-07-28 发布日期:2021-07-13
通讯作者: * 黄声富,E-mail: shengfuh2006@whu.edu.cn;李宇红,E-mail: 1004809372@whu.edu.cn
作者简介:周毅(1995~ ),女,湖北枣阳人,硕士,研究方向:牙周炎。
基金资助:
国家自然科学基金(编号:81271190);国家级大学生创新创业训练计划(编号:S2018304783)

EGCG and Baicalin Inhibit M1 Polarization of Macrophages in Mice Periodontitis through mTOR Synergistically

ZHOU Yi¹, CHEN Zhen¹, LIU Yangruoxuan¹, HU Liqun¹, YUAN Minyan¹, YAN Lu¹, HUANG Shengfu^2*, LI Yuhong^3*

1. The State Key Laboratory Breeding Base of Basic Science of Stomatology & Key laboratory of Oral Biomedicine Ministry of Education, Wuhan University, Wuhan, 430079, China;
2. Department of Orthodontics, Hospital of Stomatology, Wuhan University, Wuhan 430079, China;
3. Department of Endodontics, Hospital of Stomatology, Wuhan University, Wuhan 430079,China

Received:2020-11-16 Online:2021-07-28 Published:2021-07-13

摘要/Abstract

摘要： 目的:初步探究表没食子儿茶素没食子酸酯(epigallocatechin gallate,EGCG)和黄芩苷(baicalin)是否有协同治疗小鼠牙周炎的作用及其机制。方法:将 C57/BL6小鼠随机分为空白对照组、牙周炎组、EGCG组、EGCG+黄芩苷组。苏木精-伊红(hematoxylin-eosin,HE)染色观察牙周组织学改变和牙槽骨丧失量(alveolar bone loss,ABL);抗酒石酸酸性磷酸酶(tartrate resistant acid phosphatase,TRAP)染色观察破骨细胞数目;免疫组织化学法观察总巨噬细胞、各亚型巨噬细胞数和哺乳动物雷帕霉素靶向蛋白(mTOR)的表达。结果:丝线结扎联合局部接种牙龈卟啉单胞菌(Porphyromonas gingivalis,P.gingivalis)成功诱导小鼠实验性牙周炎。单独使用EGCG或联合使用黄芩苷减轻牙周组织炎症,减少破骨细胞数量和牙槽骨吸收量,减少牙周组织总巨噬细胞、M1型巨噬细胞数量及M1/M2比例,抑制牙周组织mTOR的表达。且EGCG和黄芩苷联合使用比单独使用EGCG效果好。结论:EGCG和黄芩苷可以协同治疗小鼠牙周炎,可能与下调mTOR信号通路抑制巨噬细胞M1向极化有关。

关键词: 表没食子儿茶素没食子酸酯, 黄芩苷, 牙周炎, 巨噬细胞, 哺乳动物雷帕霉素靶向蛋白

Abstract: Objective: To explore whether Epigallocatechin Gallate (EGCG) and baicalin have synergistic effects in the treatment of mice periodontitis and potential mechanisms. Methods: C57/BL6 mice were randomly divided into blank control group, periodontitis group, EGCG group, EGCG+ baicalin group. The changes of periodontal histology and alveolar bone absorption were observed by HE staining. TRAP staining was used to observe the number of osteoclasts. Immunohistochemistry was used to observe the number of macrophages in all subtypes and the expression of mTOR. Results: Experimental periodontitis was successfully induced by ligation of silk thread combined with local inoculation of Porphyromonas gingivalis. The use of EGCG alone or baicalin in combination reduced periodontal inflammation and alveolar bone absorption, reduce the number of osteoclasts, M1 macrophages, and the ratio of M1 and M2, inhibited the expression of mTOR in periodontal tissues, and the combined use of EGCG and baicalin was more effective than the use of EGCG alone. Conclusion: EGCG and baicalin can synergistically treat periodontitis, which may be related to the down-regulation of mTOR signaling pathway and the reduction of macrophage polarization toward M1.

Key words: epigallocatechin gallate, baicalin, periodontitis, macrophage, mTOR

周毅, 陈珍, 刘杨若萱, 胡礼群, 袁敏彦, 闫璐, 黄声富, 李宇红. EGCG和黄芩苷协同mTOR依赖性抑制小鼠牙周炎巨噬细胞M1向极化[J]. 口腔医学研究, 2021, 37(7): 622-627.

ZHOU Yi, CHEN Zhen, LIU Yangruoxuan, HU Liqun, YUAN Minyan, YAN Lu, HUANG Shengfu, LI Yuhong. EGCG and Baicalin Inhibit M1 Polarization of Macrophages in Mice Periodontitis through mTOR Synergistically[J]. Journal of Oral Science Research, 2021, 37(7): 622-627.

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EGCG和黄芩苷协同mTOR依赖性抑制小鼠牙周炎巨噬细胞M1向极化

EGCG and Baicalin Inhibit M1 Polarization of Macrophages in Mice Periodontitis through mTOR Synergistically

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