口腔医学研究

口腔医学研究 ›› 2021, Vol. 37 ›› Issue (11): 981-988.DOI: 10.13701/j.cnki.kqyxyj.2021.11.006

• 牙体牙髓病学研究 • 上一篇    下一篇

Er:YAG激光对弯曲根管内粪肠球菌灭菌效果的体外研究

何蓉, 刘学军*, 周宇琨   

  1. 郑州大学第一附属医院口腔特诊科 河南 郑州 450052
  • 收稿日期:2021-06-17 出版日期:2021-11-28 发布日期:2021-11-22
  • 通讯作者: *刘学军,E-mail:lxj@zzu.edu.cn
  • 作者简介:何蓉(1995~ ),女,安徽马鞍山人,硕士,主要从事牙体牙髓病学研究。

Sterilization Effect of Er:YAG Laser on Enterococcus Faecalis in Curved Root Canals: An in Vitro Study

HE Rong, LIU Xuejun*, ZHOU Yukun   

  1. Special Clinic Department of Stomatology, The Firist Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China
  • Received:2021-06-17 Online:2021-11-28 Published:2021-11-22

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摘要： 目的: 探究 Er:YAG激光PIPS、SWEEPS与传统冲洗方法在多根牙的直根管及弯曲根管内的灭菌效果差异。方法: 分别选取直根管和弯曲根管多根牙建立粪肠球菌感染模型。将离体牙随机分为8组,每组15例,做以下处理:A(PIPS+直根管);B(PIPS+弯曲根管);C(SWEEPS+直根管);D(SWEEPS+弯曲根管);E(PUI+直根管);F(PUI+弯曲根管);G(CI+直根管);H(CI+弯曲根管),选取同批次离体牙做空白对照。处理后提取培养细菌,24 h后菌落计数,并进行扫描电镜和激光共聚焦观察。结果: 各组灭菌效果:直根管: PIPS/SWEEPS>PUI>CI;弯曲根管组:SWEEPS>PIPS/PUI>CI;SWEEPS组在根管在根尖部能达到更高的灭菌深度及玷污层去除效果。结论: 与传统根管冲洗方法相比,Er:YAG激光PIPS、SWEEPS对弯曲根管内细菌及玷污层去除效果显著提高,且SWEEPS优于PIPS。

关键词: 铒激光, 光子引导的光声流效应, 光波增强的光声流效应, 灭菌, 玷污层

Abstract: Objective: To explore the difference in the sterilization effect of Er:YAG laser PIPS, SWEEPS, and traditional irrigation methods in straight root canals and curved root canals of multiple teeth. Methods: The straight root canal and the curved root canal were selected to establish the Enterococcus faecalis infection model. The isolated teeth were randomly divided into 8 groups, 15 cases in each group, and the following treatments were performed: A (PIPS + straight root canal); B (PIPS + curved root canal); C (SWEEPS + straight root canal); D (SWEEPS + curved root canal); E (PUI + straight root canal); F (PUI + curved root canal); G (CI + straight root canal); H (CI + curved root canal), the same batch of isolated teeth were selected as a blank control. After treatment, the bacteria were extracted and cultured. After 24 hours, the colonies were counted and observed by scanning electron microscope and laser confocal. Results: Sterilization effect of each group: straight root canal: PIPS/SWEEPS>PUI>CI; curved root canal group: SWEEPS>PIPS/PUI>CI. SWEEPS group can reach a higher sterilization depth in the root canal at the apex and smear removal effect. Conclusion: Compared with traditional root canal irrigation methods, Er:YAG laser PIPS and SWEEPS can significantly improve the removal of bacteria and smear layer in curved root canals, and SWEEPS is better than PIPS.

Key words: erbium laser, photon-initiated photoacoustic streaming, shock wave enhanced emission, sterilization, smear layer