微流控芯片在牙周领域中的应用及展望

doi:10.13701/j.cnki.kqyxyj.2022.10.005

摘要/Abstract

摘要： 微流控芯片是以分析生物和化学为研究对象,将生化领域中所涉及的样品制备、反应、分离、检测等操作集成到一块几平方厘米的芯片上,由微通道形成网络,以可控流体贯穿整个系统,用以替代常规生化实验室各种功能的一种装置。近年来,该装置在牙周疾病的诊断与治疗中的应用逐渐成为研究热点,主要涉及牙周致病菌和蛋白质生物标志物的检测、牙周病治疗药物的研发等方面,结果表明微流控芯片在牙周领域的基础研究和临床研究中发挥了重要的作用,未来有很大潜力替代传统生物实验室,成为一种新型牙周病研究实验手段。本文从微流控芯片相关概念和技术出发,对其在牙周病诊断与治疗中的应用情况做一综述,并展望其未来在牙周领域可能发挥重要作用的应用方向,为相关领域的研究人员提供试验思路与参考。

关键词: 微流控芯片, 牙周病, 即时检测, 生物膜

Abstract: Microfluidics is a kind of device, which uses analytic biology and chemistry as the research object, integrates sample preparation, reaction, separation, and detection into a piece of a few square centimeters chip, forms network by micro channel, runs through the system with controllable fluid, and replaces the test of conventional biochemical laboratory. In recent years, the device has gradually become a hot research issue in the diagnosis and treatment of periodontal disease, mainly related to periodontal pathogens and protein biomarkers detection, and drug research and development of periodontal therapy. A large number of studies have shown that microfluidics plays an important role in basic research and clinical research in the field of periodontics. In addition, it has great potential to replace traditional biological laboratories and become a novel periodontal disease research method in the future. Starting from the related concepts and technologies of microfluidics, this paper reviews the application of microfluidics in the diagnosis and treatment of periodontal disease, and looks forward to its possible application directions in the periodontal field in the future, so as to provide experimental ideas and references for researchers in related fields.

Key words: microfluidics, periodontal disease, point-of-care testing, biofilm

刘恩言, 丁一. 微流控芯片在牙周领域中的应用及展望[J]. 口腔医学研究, 2022, 38(10): 926-929.

LIU Enyan, DING Yi. Application and Prospect of Microfluidics in the Field of Periodontics[J]. Journal of Oral Science Research, 2022, 38(10): 926-929.

参考文献

[1] 束蓉.牙周病病因及治疗研究进展[J].上海交通大学学报(医学版),2007,27(6):625-628.
[2] Sadrameli M, Bathini P, Alberi L. Linking mechanisms of periodontitis to Alzheimer's disease[J]. Curr Opin Neurol, 2020, 33(2):230-238.
[3] Liccardo D, Cannavo A, Spagnuolo G, et al. Periodontal disease: A risk factor for diabetes and cardiovascular disease[J]. Int J Mol Sci, 2019, 20(6):1414.
[4] Choi SE, Sima C, Pandya A. Impact of treating oral disease on preventing vascular diseases: A model-based cost-effectiveness analysis of periodontal treatment among patients with type 2 diabetes[J]. Diabetes care, 2020, 43(3):563-571.
[5] Nguyen T, Zoëga Andreasen S, Wolff A, et al. From lab on a chip to point of care devices: the role of open source microcontrollers[J]. Micromachines, 2018, 9(8):403.
[6] Li X, Yang X, Liu L, et al. A microarray platform designed for high-throughput screening the reaction conditions for the synthesis of micro/nanosized biomedical materials[J]. Bioact Mater, 2020, 5(2):286-296.
[7] 李晓琼,杨春华,潘邵武,等.面向 POCT 应用的微流控芯片技术综述[J].世界复合医学,2015,1(1):30-37.
[8] Zhu H, Fohlerová Z, Pekárek J, et al. Recent advances in lab-on-a-chip technologies for viral diagnosis[J]. Biosens Bioelectron,2020,153:112041.
[9] Zhao Y, Demirci U, Chen Y, et al. Multiscale brain research on a microfluidic chip[J]. Lab Chip, 2020, 20(9):1531-1543.
[10] Sierra J, Marrugo-Ramirez J, Rodríguez-Trujillo R, et al.Sensor-integrated microfluidic approaches for liquid biopsies applications in early detection of cancer[J]. Sensors (Basel), 2020, 20(5):1317.
[11] Jigar Panchal H, Kent NJ, Knox AJS, et al. Microfluidics in haemostasis: A review[J]. Molecules, 2020, 25(4):833.
[12] Nghe Ph, Terriac E, Schneider M, et al. Microfluidics and complex fluids[J]. Lab Chip, 2011, 11(5):788-794.
[13] Beyer-Hans KM, Sigrist MW, Silbereisen A, et al. Salivary fingerprinting of periodontal disease by infrared-ATR spectroscopy[J]. Proteomics Clin Appl, 2020, 14(3):e1900092.
[14] Herr AE, Hatch AV, Giannobile WV, et al. Integrated microfluidic platform for oral diagnostics[J]. Ann N Y Acad Sci, 2007, 1098:362-374.
[15] Curtis MA, Diaz PI, Van Dyke TE. The role of the microbiota in periodontal disease[J]. Periodontol 2000, 2020, 83(1):14-25.
[16] Papapanou PN, Engebretson SP, Lamster IB. Current and future approaches for diagnosis of periodontal diseases[J]. N Y State Dent J, 1999, 65(4):32-37.
[17] Li Z, Ju R, Sekine S, et al. All-in-one microfluidic device for on-site diagnosis of pathogens based on an integrated continuous flow PCR and electrophoresis biochip[J]. Lab Chip, 2019, 19(16):2663-2668.
[18] Hartenbach FARR, Velasquez É, Nogueira FCS, et al. Proteomic analysis of whole saliva in chronic periodontitis[J]. J Proteomics, 2020, 213:103602.
[19] Herr AE, Hatch AV, Throckmorton DJ, et al. Microfluidic immunoassays as rapid saliva-based clinical diagnostics[J]. Proc Natl Acad Sci U S A, 2007, 104(13):5268-5273.
[20] Boelen GJ, Boute L, d'Hoop J, et al. Matrix metalloproteinases and inhibitors in dentistry[J]. Clin Oral Investig, 2019, 23(7):2823-2835.
[21] Kharaeva ZF, Mustafaev MS, Khazhmetov AV, et al. Anti-bacterial and anti-inflammatory effects of toothpaste with Swiss medicinal herbs towards patients suffering from gingivitis and initial stage of periodontitis: from clinical efficacy to mechanisms[J]. Dent J (Basel), 2020, 8(1):10.
[22] Wang HW, Lai EH, Yang CN, et al. Intracanal metformin promotes healing of apical periodontitis via suppressing inducible nitric oxide synthase expression and monocyte recruitment[J]. J Endod, 2020, 46(1):65-73.
[23] Bhakta SA, Borba R, Taba M Jr, et al. Determination of nitrite in saliva using microfluidic paper-based analytical devices[J]. Anal Chim Acta, 2014, 809:117-122.
[24] 俞文伟.浅谈牙周病发生的常见因素及治疗进展[J].名医,2020,(1):55.
[25] Brann M, Suter JD, Addleman RS, et al. Monitoring bacterial biofilms with a microfluidic flow chip designed for imaging with white-light interferometry[J]. Biomicrofluidics, 2017, 11(4):044113.
[26] Belibasakis GN, Kast JI, Thurnheer T, et al. The expression of gingival epithelial junctions in response to subgingival biofilms[J]. Virulence, 2015, 6(7):704-709.
[27] 张艺龄,徐杰.基因芯片在牙周微生物检测中的研究进展[J].医学综述,2020,26(21):4190-4194.
[28] 李俊吉.基于微流控技术的微量核酸高通量测序样本制备应用研究[D].东南大学,2018.
[29] Lee S, Chen D, Park M, et al. Single cell RNA sequencing analysis of human dental pulp stem cells and human periodontal ligament stem cells[J]. J Endod, 2021, 48(2):240-248.
[30] Chakrabarty S, Quiros-Solano WF, Kuijten MM, et al. A microfluidic cancer-on-chip platform predicts drug response using organotypic tumor slice culture[J]. Cancer Res, 2022, 82(3):510-520.
[31] Loo J, Sicher I, Goff A, et al. Microfluidic transfection of mRNA into human primary lymphocytes and hematopoietic stem and progenitor cells using ultra-fast physical deformations[J]. Sci Rep, 2021, 11(1):21407.
[32] Stucki A, Jusková P, Nuti N, et al. Synchronized reagent delivery in double emulsions for triggering chemical reactions and gene expression[J]. Small Methods, 2021, 5(8):e2100331.
[33] Payne K, Brooks JM, Taylor GS, et al. Immediate sample fixation increases circulating tumour cell (CTC) capture and preserves phenotype in head and neck squamous cell carcinoma: Towards a standardised spproach to microfluidic CTC biomarker discovery[J]. Cancers (Basel), 2021, 13(21):5519.
[34] Lim M, Park S, Jeong HO, et al. Circulating tumor cell clusters are cloaked with platelets and correlate with poor prognosis in unresectable pancreatic cancer[J]. Cancers (Basel), 2021, 13(21):5272.
[35] Obermayr E, Koppensteiner N, Heinzl N, et al. Cancer stem cell-like circulating tumor cells are prognostic in non-small cell lung cancer[J]. J Pers Med, 2021, 11(11):1225.