壳聚糖/姜黄素纳米复合物对变异链球菌致龋力的影响

doi:10.13701/j.cnki.kqyxyj.2022.11.012

口腔医学研究 ›› 2022, Vol. 38 ›› Issue (11): 1058-1063.DOI: 10.13701/j.cnki.kqyxyj.2022.11.012

壳聚糖/姜黄素纳米复合物对变异链球菌致龋力的影响

梅杰, 施婉娓, 郭婷, 吴敏婧^*

南方医科大学口腔医院广东广州 510250

收稿日期:2022-04-18 出版日期:2022-11-25 发布日期:2022-11-22
通讯作者: *吴敏婧,E-mail:837427505@qq.com
作者简介:梅杰(1992~ ),男,重庆人,博士,主治医师,研究方向:口腔微生物。
基金资助:
南方医科大学口腔医院院级科研培育项目(编号:PY2021022)

Effect of Chitosan/curcumin Nanocomposite on Cariogenic Ability of Streptococcus Mutans

MEI Jie, SHI Wanwei, GUO Ting, WU Minjing^*

Stomatological Hospital, Southern Medical University, Guangzhou 510250, China

Received:2022-04-18 Online:2022-11-25 Published:2022-11-22

摘要/Abstract

摘要： 目的: 探讨壳聚糖/姜黄素纳米复合物对变异链球菌致龋力的影响。方法: 将姜黄素与壳聚糖复合后形成含有不同浓度(0、0.01、0.05、0.10 mmol/L)姜黄素的壳聚糖/姜黄素纳米复合物(CS-Cur)。将CS-Cur作用于浮游态及生物膜状态下的变异链球菌,观察变异链球菌生长增殖曲线、培养物pH变化、细菌糖酵解能力,结晶紫染色评估变异链球菌生物膜的量,蒽酮法检测生物膜内非水溶性胞外多糖含量,菌落计数评估生物膜中所含细菌的量。通过以上实验评估变异链球菌在CS-Cur作用后致龋因子表达的变化。结果: CS-Cur作用于变异链球菌后,其生长增殖受到了抑制,该抑制随着Cur浓度升高而增强;变异链球菌培养物pH也无法下降至空白对照组水平;同时变异链球菌的糖酵解能力在CS-Cur作用后也有所下降;CS-Cur作用于变异链球菌后,结晶紫染色结果显示:变异链球菌所形成的生物膜的量下降(P<0.05);蒽酮检测胞外多糖结果显示:生物膜中所含的非水溶性胞外多糖显著减少(P<0.05);菌落计数结果显示:细菌数量也显著减少(P<0.05)。结论: CS-Cur纳米复合物对变异链球菌致龋因子的表达具有显著抑制作用,提示CS-Cur纳米复合物有望成为新的防龋药物,为龋病预防和治疗提供新思路。

关键词: 壳聚糖, 姜黄素, 变异链球菌, 致龋因子

Abstract: Objective: To investigate the effect of chitosan/curcumin nanocomposite on cariogenic ability of Streptococcus mutans (S. mutans). Methods: Curcumin was compounded with chitosan to form chitosan/curcumin nanocomposites (CS/cur) containing different concentrations of curcumin (0, 0.01, 0.05, and 0.10 mmol/L). CS/cur was applied to S. mutans in planktonic state and biofilm state. The growth curve of S. mutans, pH change of culture, and bacterial glycolysis ability were evaluated. Crystal violet staining was utilized to evaluate the amount of S. mutans biofilm biomass, and anthrone method was used to detect the content of insoluble extracellular polysaccharide in biofilm. Finally, colony count was used to evaluate the number of bacteria in biofilm. Results: CS/cur inhibited the growth of S. mutans, and the inhibition effect of CS/cur was cur concentration related. The pH of S. mutans culture could not be reduced to the level of blank control group. Moreover, the glycolytic ability of S. mutans also decreased after CS/cur. After CS/cur was applied to S. mutans, crystal violet staining showed that the amount of biofilm formed by S. mutans decreased (P<0.05). The results of anthrone detection of extracellular polysaccharides showed that the content of insoluble extracellular polysaccharides in biofilm decreased significantly (P<0.05). Colony count showed that the number of bacteria also decreased significantly (P<0.05). Conclusion: CS/cur nanocomposite has a significant inhibitory effect on the expression of cariogenic factors of S. mutans, suggesting that CS/cur nanocomposite is expected to become a new anti-caries drug and provides a new strategy for caries prevention and treatment.

Key words: chitosan, curcumin, streptococcus mutans, cariogenic factor

梅杰, 施婉娓, 郭婷, 吴敏婧. 壳聚糖/姜黄素纳米复合物对变异链球菌致龋力的影响[J]. 口腔医学研究, 2022, 38(11): 1058-1063.

MEI Jie, SHI Wanwei, GUO Ting, WU Minjing. Effect of Chitosan/curcumin Nanocomposite on Cariogenic Ability of Streptococcus Mutans[J]. Journal of Oral Science Research, 2022, 38(11): 1058-1063.

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壳聚糖/姜黄素纳米复合物对变异链球菌致龋力的影响

Effect of Chitosan/curcumin Nanocomposite on Cariogenic Ability of Streptococcus Mutans

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