miR-485-5p靶向RAPTOR影响口腔鳞状细胞癌迁移、侵袭和增殖

doi:10.13701/j.cnki.kqyxyj.2024.05.010

口腔医学研究 ›› 2024, Vol. 40 ›› Issue (5): 429-435.DOI: 10.13701/j.cnki.kqyxyj.2024.05.010

miR-485-5p靶向RAPTOR影响口腔鳞状细胞癌迁移、侵袭和增殖

陈嘉雯^1,2, 丁啸², 栾可峰¹, 王高君¹, 李洪利³, 胡温庭¹, 孙学辉^1*

1.山东第二医科大学附属医院口腔科山东潍坊 261000;
2.山东第二医科大学口腔医学院山东潍坊 261053;
3.山东第二医科大学医学研究实验中心山东潍坊 261053

收稿日期:2024-01-05 出版日期:2024-05-28 发布日期:2024-05-22
通讯作者: * 孙学辉,E-mail: sunxuehui163@163.com
作者简介:陈嘉雯(1998~ ),女,湖南桃江县人,硕士在读,研究方向:口腔鳞状细胞癌的侵袭与迁移。
基金资助:
山东省自然科学基金(编号:ZR202110190030);潍坊医学院附属医院种子基金(编号:2021wffyzzjj06);潍坊市科技发展计划项目(编号:2022YX029)

RAPTOR Affects Migration, Invasion, and Proliferation of Oral Squamous Cell Carcinoma through miR-485-5p

CHEN Jiawen^1,2, DING Xiao², LUAN Kefeng¹, WANG Gaojun¹, LI Hongli³, HU Wenting¹, SUN Xuehui^1*

1. Department of Stomatology, Affiliated Hospital of Shandong Second Medical University, Weifang 261000, China;
2. School of Stomatology, Shandong Second Medical University, Weifang 261053, China;
3. Medical Research and Experiment Center, Shandong Second Medical University, Weifang 261053, China

Received:2024-01-05 Online:2024-05-28 Published:2024-05-22

摘要/Abstract

摘要： 目的:探讨mTOR调节相关蛋白(regulatory-associated protein of mTOR, RAPTOR)对口腔鳞状细胞癌(oral squamous cell carcinoma,OSCC)迁移、侵袭和增殖能力的影响。方法:利用TCGA生物信息数据库查询在头颈部鳞状细胞癌(head and neck squamous cell carcinoma,HNSCC)组织与癌旁组织中差异表达的mRNA。Western blot检测RAPTOR在人口腔上皮细胞HOEC和OSCC细胞系中的表达。利用伤口愈合实验、Transwell实验、EdU实验检测各组细胞迁移、侵袭及增殖能力。生物信息学网站预测与RAPTOR靶向结合的微小RNA(microRNA,miR)。功能学实验验证miR-485-5p是否可以靶向RAPTOR影响OSCC细胞的迁移、侵袭和增殖。结果:RAPTOR在HNSCC组织中较癌旁组织表达增高。伤口愈合、Transwell和EdU实验结果示,RAPTOR有促进OSCC细胞的迁移、侵袭和增殖能力。miR-485-5p能与RAPTOR靶向结合,且miR-485-5p上调能逆转RAPTOR促进CAL27细胞迁移、侵袭和增殖能力。结论:miR-485-5p通过靶向RAPTOR抑制OSCC细胞迁移、侵袭和增殖能力。

Abstract: Objective: To investigate the effect of regulatory-associated protein of mTOR (RAPTOR) on the migration, invasion, and proliferation of oral squamous cell carcinoma (OSCC). Methods: The TCGA database was used to identify differentially expressed mRNA in head and neck squamous cell carcinoma (HNSCC) tissues and adjacent tissues. Western blot assay detected RAPTOR expression in HOEC and OSCC cell lines. Cell migration ability was detected by the wound healing experiment. Transwell assay and EdU were used to detect the cell invasion and cell proliferation ability. Bioinformatics websites were used to predict microRNA binding to RAPTOR. Functional tests were conducted to determine if miR-485-5p could impact cell migration, invasion, and proliferation by targeting RAPTOR. Results: RAPTOR expression was higher in HNSCC tissues compared to adjacent tissues. Wound healing, Transwell, and EdU experiments showed that RAPTOR promoted the migration, invasion, and proliferation of OSCC cells. Up-regulation of miR-485-5p can reverse the promoting effect of RAPTOR on the migration, invasion, and proliferation of CAL27 cells. Conclusion: miR-485-5p inhibits OSCC cell migration, invasion, and proliferation by targeting RAPTOR.

Key words: RAPTOR, miR-485-5p, oral squamous cell carcinoma, migration, invasion, proliferation

陈嘉雯, 丁啸, 栾可峰, 王高君, 李洪利, 胡温庭, 孙学辉. miR-485-5p靶向RAPTOR影响口腔鳞状细胞癌迁移、侵袭和增殖[J]. 口腔医学研究, 2024, 40(5): 429-435.

CHEN Jiawen, DING Xiao, LUAN Kefeng, WANG Gaojun, LI Hongli, HU Wenting, SUN Xuehui. RAPTOR Affects Migration, Invasion, and Proliferation of Oral Squamous Cell Carcinoma through miR-485-5p[J]. Journal of Oral Science Research, 2024, 40(5): 429-435.

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miR-485-5p靶向RAPTOR影响口腔鳞状细胞癌迁移、侵袭和增殖

RAPTOR Affects Migration, Invasion, and Proliferation of Oral Squamous Cell Carcinoma through miR-485-5p

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