口腔医学研究

口腔医学研究 ›› 2026, Vol. 42 ›› Issue (4): 293-299.DOI: 10.13701/j.cnki.kqyxyj.2026.04.005

• 口腔种植学研究 • 上一篇    下一篇

负载盐酸小檗碱和羟基磷灰石复合涂层的3D打印钛合金表面体外成骨及抗菌性能研究

魏昭明1,2, 宋娜玮1,2, 闫星华1,2, 韩泽奎1,2, 刘苗1,2*   

  1. 1.佳木斯大学附属口腔医院,佳木斯大学口腔医学院 黑龙江 佳木斯 154002;
    2.黑龙江省口腔生物医学材料及临床应用重点实验室,佳木斯大学口腔医学工程实验中心 黑龙江 佳木斯 154002
  • 收稿日期:2025-08-21 发布日期:2026-04-23
  • 通讯作者: *刘苗,E-mail:23160118@qq.com
  • 作者简介:魏昭明(1998~ ),男,辽宁辽阳人,硕士,医师,研究方向:种植体材料。
  • 基金资助:
    黑龙江省省属高等学校基本科研业务费科研项目(编号:2018-KYYWF-0933)

Study on Osteogenesis and Antibacterial Properties of 3D Printed Titanium Alloy Coated with Berberine Hydrochloride and Hydroxyapatite in Vitro

WEI Zhaoming1,2, SONG Nawei1,2, YAN Xinghua1,2, HAN Zekui1,2, LIU Miao1,2*   

  1. 1. Affiliated Stomatological Hospital of Jiamusi University, School of Stomatology of Jiamusi University, Jiamusi 154002, China;
    2. Heilongjiang Province Key Laboratory of Oral Biomedical Materials and Clinical Applications, Jiamusi University Dental Engineering Experimental Center, Jiamusi 154002, China
  • Received:2025-08-21 Published:2026-04-23

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摘要： 目的:将盐酸小檗碱(berberine hydrochloride,BBH)和羟基磷灰石(hydroxyapatite,HA)负载至经酸蚀喷砂处理后的钛合金(Ti6Al4V)的表面,以改善Ti6Al4V的生物相容性。方法:3D打印Ti6Al4V试件,酸蚀喷砂处理后将其分为喷砂酸蚀(sandblasted, large-grit, acid-etched,SLA,对照组)、HA(负载HA)、BBH(负载BBH)、BBH/HA(负载复合涂层),采用电化学沉积法在各组表面制备BBH/HA复合涂层,通过扫描电镜观察涂层的微观形貌,通过接触角测量仪和粗糙度测量仪等检测其亲水性能、粗糙度等表面表征,利用平板计数法分析各组对金黄色葡萄球菌和大肠杆菌的抑菌情况,以及通过细胞增殖试验检测各组材料对MC3T3-E1细胞增殖的影响以及通过茜素红染色和荧光定量聚合酶链反应实验分析试件的成骨能力。结果:微观形貌观察结果显示各涂层组表面涂层较为均匀,与基底结合较牢固,BBH/HA组在亲水性、细胞增殖和抗菌性等方面均优于其他各组。结论:BBH/HA负载至经喷砂酸蚀后Ti6Al4V的表面可提高其表面的成骨和抗菌能力,对其未来在临床种植修复的应用提供了新思路。

关键词: 3D打印, Ti6Al4V, 盐酸小檗碱, 羟基磷灰石, 涂层

Abstract: Objective: To improve the biocompatibility of Ti6Al4V by loading berberine hydrochloride (BBH) and hydroxyapatite (HA) on the surface after acid blasting. Methods: 3D printed Ti6Al4V samples were divided into SLA (control), HA (HA loaded), BBH (BBH loaded), and BBH/HA (BBH/HA loaded composite coating) groups after acid etching and sandblasting. BBH/HA composite coating was prepared on titanium alloy surface by electrochemical deposition method. The microstructure of the coating was observed by scanning electron microscope. The hydrophilic property and roughness of the coating were detected by contact angle measuring instrument and roughness measuring instrument. The bacteriostasis of each group to Staphylococcus aureus and Escherichia coli was analyzed by plate counting method. The effect on MC3T3-E1 cell proliferation was detected by cell proliferation test. The osteogenic ability of each sample was analyzed by alizarin red staining and quantitative pcr test. Results: The microscopic morphology observation results showed that the surface of each coating group was relatively uniform and firmly bonded to the substrate. The BBH/HA group was superior to other groups in terms of hydrophilicity, cell proliferation, and antibacterial activity. Conclusion: Berberine hydrochloride and hydroxyapatite loaded on the surface of Ti6Al4V after sandblasting can improve the osteogenic and antibacterial ability.

Key words: 3D printing, Ti6Al4V, berberine hydrochloride, hydroxyapatite, coating