甘肃东乡及裕固族牙周炎口腔微生物群落结构研究

口腔医学研究 ›› 2015, Vol. 31 ›› Issue (4): 365-369.

甘肃东乡及裕固族牙周炎口腔微生物群落结构研究

焦康礼¹, 朱玉娟¹, 谢沛原¹, 李俊平¹, 吴芳¹, 周建业², 李志强^2*, 余占海^1*

1. 兰州大学口腔医学院甘肃兰州 730000;
2. 西北民族大学口腔医学国家民委重点实验室

收稿日期:2014-09-12 出版日期:2015-04-28 发布日期:2016-04-29
通讯作者: 李志强,E-mail: xbmulzq@163.com;余占海,E-mail: yuzhanhai@lzu.edu.cn
作者简介:焦康礼(1980~ ),男,甘肃人,硕士,主要从事口腔修复及口腔微生物群落结构多样性研究。
基金资助:
国家自然基金项目(编号:31360124/C0309、;31160124/C0309)

Oral Microbial Community Structure of Periodontitis Patients between Dongxiang and Yugurs in Gansu Province.

JIAO Kang-li, ZHU Yu-juan, XIE Pei-yuan, et al

School of Stomatology, Lanzhou University, Lanzhou 730000

Received:2014-09-12 Online:2015-04-28 Published:2016-04-29

摘要/Abstract

摘要： 目的:研究甘肃东乡族和裕固族成年人牙周炎唾液微生物群落结构。方法:构建16S rRNA克隆文库对测序结果进行分析,DP(东乡族样本),YP(裕固族样本)。结果:发现链球菌属存在于所有样本中;伴放线放线杆菌属(DP:0.00%, YP:3.30%)、奈瑟菌属(DP:10.40%, YP:3.60%)及韦永球菌属(DP:0.40%, YP:3.85%)在两组样本中的分布具有明显的差异(P＜0.05)。结论:甘肃东乡及裕固族牙周炎口腔微生物群落结构存在一定差异,其中,链球菌属、普氏菌属、梭杆菌属及嗜血杆菌属在牙周炎患者口腔中的作用值得进一步研究。

关键词: 东乡族, 裕固族, 16S, rRNA

Abstract: Objective: To study the salivary microbial community structure in adult periodontitis patients between Dongxiang and Yugurs in Gansu province. Methods: By constructing 16S rRNA gene clone library, we ananlyzed the sequencing results of DP(Dongxiang periodontitis samples) and YP(Yugurs periodontitis samples). Results: Streptococcus were detected in all 10 samples. There was a significant difference (P<0.05) of Actinobacillus (DP: 0.00%, YP: 3.30%), Neisseria (DP: 10.40%, YP: 3.60%) and Veillonella (DP: 0.40% , YP: 3.85%) between the two groups. Conclusion: There might be a certain difference of oral microbial community structure between Dongxiang and Yugurs periodontitis. And the roles of Streptococcus, Actinobacillus, Neisseria and Veillonella in the oral cavity of periodontitis patients may deserve further study.

Key words: Dongxiang , Yugurs 16S , rRNA , Clone library , Dominant bacteria genera

中图分类号:

R781.4

焦康礼, 朱玉娟, 谢沛原, 李俊平, 吴芳, 周建业, 李志强, 余占海. 甘肃东乡及裕固族牙周炎口腔微生物群落结构研究[J]. 口腔医学研究, 2015, 31(4): 365-369.

JIAO Kang-li, ZHU Yu-juan, XIE Pei-yuan, et al. Oral Microbial Community Structure of Periodontitis Patients between Dongxiang and Yugurs in Gansu Province.[J]. Journal of Oral Science Research, 2015, 31(4): 365-369.

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