口腔医学研究

口腔医学研究 ›› 2015, Vol. 31 ›› Issue (9): 894-897.

• 临床研究论著 • 上一篇    下一篇

正畸牙移动过程中高迁移率族蛋白B1的改变

许潾于*,彭昕欣,雷浪,黄鹏程,张端强   

  1. 福建医科大学附属口腔医院正畸科 福建 福州 350002
  • 收稿日期:2015-06-25 出版日期:2015-09-28 发布日期:2016-05-04
  • 通讯作者: 许潾于,电话:0591-83736432
  • 作者简介:许潾于(1971~ ),男,福建福州人,硕士,副教授,主要从事错合畸形的病因及发病机理的研究。
  • 基金资助:
    福建省自然科学基金面上项目(编号:2014J01318)

Expression of High Mobility Group Box-1 during Orthodontic Tooth Movement

XU Lin-yu1, PENG Xin-xin1, LEI Lang2, HUANG Peng-cheng1, ZHANG Rui-qiang1   

  1. Department of Orthodontics, Stomatology Hospital, Fujian Medical University, Fuzhou 350002, China
  • Received:2015-06-25 Online:2015-09-28 Published:2016-05-04

PDF (PC)

280

摘要： 目的:研究高迁移率族蛋白B1(High Mobility Group Box-1,HMGB1)在正畸牙移动过程中的表达,以指导正畸临床加力。方法:30只Wistar大鼠,分为0、1、3、5和7 d组,每组6只;左侧施加0.6 N以近中移动上颌第一磨牙,右侧放置正畸装置但不加力作为对照;HE染色观察正畸牙移动过程中牙周组织的变化,免疫组织化学染色观察牙周支持组织中HMGB1的变化,以平均光密度法检测组织中HMGB1的表达量。结果:HE染色显示,加力1 d~7 d,大鼠第一磨牙牙周组织呈现正畸牙移动的典型变化:压力侧牙周膜变窄,呈现纤维排列紊乱,破骨细胞数目逐渐增多;张力侧牙周膜增宽,纤维排列整齐,成骨细胞数目逐渐增多。免疫组织化学染色显示,压力侧1 d时HMGB1表达量最低(0.0012 ± 0.0009),显著低于1 d对照组(0.0239 ± 0.011),从1~7 d,压力侧HMGB1逐渐增加,到7 d时到达加力后最高值(0.0127 ± 0.0014);张力侧1 d时HMGB1表达量(0.0193 ± 0.0012)低于1 d对照组(0.0284 ± 0.009),到5 d时,张力侧HMGB1达到最高值(0.0324 ± 0.0014),但与5 d对照组(0.0289 ± 0.0012)无显著差别。结论:过大的正畸矫治力减少压力侧牙周组织内HMGB1的表达,减慢透明样组织清除和正畸牙移动。

关键词: 正畸牙移动, 高迁移率族蛋白B1, 骨改建

Abstract: Objective: To investigate the role of high mobility group box-1 (HMGB1) during orthodontic tooth movement. Methods: Thirty Wistar rats were randomly divided into 0, 1, 3, 5 and 7d group. Left maxillary 1st molars were protracted by a force of 0.6N, whereas orthodontic appliances were placed but orthodontic force was not applied on the contral lateral 1st molars. HE staining was utilized to observe the morphology of periodontal tissue during tooth movement, and immunohistology was used to detect HMGB1 expression by evaluating average optical density. Results: Typical histological features were observed in the periodontal tissue around upper molars, with narrowed periodontal ligament, disarranged periodontal fibers and increased osteoclasts in compression side, and widened periodontal membrane, extended periodontal fibers and increased osteoblasts in the tensile side. Expression of HMGB1 reached the lowest point (0.0012±0.0009) on 1st day on the compression side, which was significantly lower than control (0.0239±0.011), and increased gradually from 1st day to 7th day, peaking at 0.0127±0.0014 on 7th day. Expression of HMGB1 decreased on the 1st day (0.0193±0.0012) on the tensile side, which was lower than control group (0.0284 ± 0.009), and reached the highest point on 5th day (0.0324±0.0014), which was not significantly different from control group (0.0289±0.0012). Conclusion: Excessive orthodontic force reduced HMGB1 expression in periodontal membrane and decreased hyaline tissue clearance and tooth movement.

Key words: Orthodontic tooth movement, High mobility group box-1, Bone remodeling

中图分类号: