共培养诱导人脐带间充质干细胞(hUCMSCs)向牙周膜细胞(hPDLs)分化的体外实验研究

口腔医学研究 ›› 2015, Vol. 31 ›› Issue (8): 786-.

共培养诱导人脐带间充质干细胞(hUCMSCs)向牙周膜细胞(hPDLs)分化的体外实验研究

李云鹏¹,赵昕²,王一波¹,刘彦普^1*,侯锐^1*

1. 第四军医大学口腔医院口腔颌面外科,军事口腔医学国家重点实验室陕西西安 710032;
2. 第四军医大学西京医院内分泌代谢科

收稿日期:2015-01-26 出版日期:2015-08-28 发布日期:2016-05-04
通讯作者: 刘彦普,E-mail:liuyanpu@fmmu.edu.cn;侯锐,电话:029-84776102
作者简介:李云鹏(1980~ ),男,陕西人,主治医师,博士,主要从事牙周组织工程缺损与修复的研究工作。
基金资助:
国家自然科学基金资助项目(编号:81100792)

Differentiation of Human Umbilical Cord Mesenchymal Stem Cells into Human Periodontal Ligament Cell by Co-culture in Vitro

LI Yun-peng, ZHAO Xin, WANG Yi-bo, et al

Department of Oral and Maxillofacial Surgery, State Key Laboratory of Military Stomatology, School of Stomatology, The Fourth Military Medical University, Xian 710032

Received:2015-01-26 Online:2015-08-28 Published:2016-05-04

摘要/Abstract

摘要： 目的:研究人脐带间充质干细胞(hUCMSCs)向人牙周膜细胞(hPDLs)分化的可能性。方法:原代培养获得hUCMSCs和hPDLs;应用Transwell小室将两种细胞进行非接触式共培养;使用流式细胞仪检测共培养过程中hUCMSCs干细胞标记物CD146、SOX2、SSEA和Stro-1的变化情况;通过免疫荧光技术和Western Blot技术检测共培养过程中波形蛋白(Vimentin)在hUCMSCs中的变化情况。结果:共培养过程中hUCMSCs中的CD146、SOX2、SSEA和Stro-1持续降低;而Vimentin持续增高。结论:通过与hPDLs共培养,可以成功地将hUCMSCs诱导分化成为牙周膜样细胞,进一步证明了hUCMSCs可用于牙周损伤修复的可能。

关键词: 脐带间充质干细胞, 牙周膜细胞, 共培养

Abstract: Objective: To investigate the ability of human umbilical cord mesenchymal stem cells (hUCMSCs) differentiation into human periodontal ligament cells (hPDLs) by co-culture in vitro. Methods: The hUCMSCs and hPDLs were obtained by primary culture. hUCMSCs and hPDLs were indirectly cocultured in transwell plates. The stemness markers such as CD146, sex determining region Y-Box 2 (SOX2), stage-specific embryonic antigen (SSEA), and Stro-1 changes were detected by flow cytometry after coculture for 0, 1 and 3 weeks, respectively. The Vimentin expression was determined by immunofluorescence stain and Western blot. Results: During the co-culture process, the expression of stemness markers in hUCMSCs decreased gradually, while the Vimentin expression was on the contrary. Conclusion: The hUCMSCs had the potential to differentiate into hPDLs under co-culture condition, which suggested that it could be used for periodontal repair in the future.

Key words: Umbilical cord mesenchymal stem cells, Periodontal ligament cells, Co-culture

中图分类号:

R329.2

李云鹏,赵昕,王一波,刘彦普,侯锐. 共培养诱导人脐带间充质干细胞(hUCMSCs)向牙周膜细胞(hPDLs)分化的体外实验研究[J]. 口腔医学研究, 2015, 31(8): 786-.

LI Yun-peng, ZHAO Xin, WANG Yi-bo, et al. Differentiation of Human Umbilical Cord Mesenchymal Stem Cells into Human Periodontal Ligament Cell by Co-culture in Vitro[J]. Journal of Oral Science Research, 2015, 31(8): 786-.

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